A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Abstract We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The froz...

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Autores principales:	Yuki Tajika, Tohru Murakami, Keiya Iijima, Hiroki Gotoh, Maiko Takahashi-Ikezawa, Hitoshi Ueno, Yuhei Yoshimoto, Hiroshi Yorifuji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39420d2110864fbfa708ae13587f6437
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spelling	oai:doaj.org-article:39420d2110864fbfa708ae13587f64372021-12-02T16:07:43ZA novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging10.1038/s41598-017-03900-92045-2322https://doaj.org/article/39420d2110864fbfa708ae13587f64372017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03900-9https://doaj.org/toc/2045-2322Abstract We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen section can be used for conventional light microscopic analysis to obtain 2-dimensional (2D) anatomical and molecular information, while serial block-face images can be used as 3-dimensional (3D) volume data for anatomical analysis. Thus, the sections maintain positional information in the specimen, and allows the correlation of 2D microscopic data and 3D volume data in a single specimen. The subjects can vary in size and type, and can cover most specimens encountered in biology. In addition, the required system for our method is characterized by cost-effectiveness. Here, we demonstrated the utility of CoMBI using specimens ranging in size from several millimeters to several centimeters, i.e., mouse embryos, human brainstem samples, and stag beetle larvae, and present successful correlation between the 2D light microscopic images and 3D volume data in a single specimen.Yuki TajikaTohru MurakamiKeiya IijimaHiroki GotohMaiko Takahashi-IkezawaHitoshi UenoYuhei YoshimotoHiroshi YorifujiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Tajika Tohru Murakami Keiya Iijima Hiroki Gotoh Maiko Takahashi-Ikezawa Hitoshi Ueno Yuhei Yoshimoto Hiroshi Yorifuji A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging
description	Abstract We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen section can be used for conventional light microscopic analysis to obtain 2-dimensional (2D) anatomical and molecular information, while serial block-face images can be used as 3-dimensional (3D) volume data for anatomical analysis. Thus, the sections maintain positional information in the specimen, and allows the correlation of 2D microscopic data and 3D volume data in a single specimen. The subjects can vary in size and type, and can cover most specimens encountered in biology. In addition, the required system for our method is characterized by cost-effectiveness. Here, we demonstrated the utility of CoMBI using specimens ranging in size from several millimeters to several centimeters, i.e., mouse embryos, human brainstem samples, and stag beetle larvae, and present successful correlation between the 2D light microscopic images and 3D volume data in a single specimen.
format	article
author	Yuki Tajika Tohru Murakami Keiya Iijima Hiroki Gotoh Maiko Takahashi-Ikezawa Hitoshi Ueno Yuhei Yoshimoto Hiroshi Yorifuji
author_facet	Yuki Tajika Tohru Murakami Keiya Iijima Hiroki Gotoh Maiko Takahashi-Ikezawa Hitoshi Ueno Yuhei Yoshimoto Hiroshi Yorifuji
author_sort	Yuki Tajika
title	A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging
title_short	A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging
title_full	A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging
title_fullStr	A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging
title_full_unstemmed	A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging
title_sort	novel imaging method for correlating 2d light microscopic data and 3d volume data based on block-face imaging
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/39420d2110864fbfa708ae13587f6437
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A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

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