Calmodulin regulates the olfactory performance in Drosophila melanogaster

Abstract Insect odorant receptors (ORs) detect volatile chemical cues with high sensitivity. These ORs operate as ligand-gated ion channels and are formed by heptahelical OrX and Orco (co-receptor) proteins. A highly conserved calmodulin (CaM) binding site (CBS) 336SAIKYWVER344 within the second int...

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Autores principales:	Kalpana Jain, Sofia Lavista-Llanos, Veit Grabe, Bill S. Hansson, Dieter Wicher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3948fd547a284979b2392ce5fa908af7
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spelling	oai:doaj.org-article:3948fd547a284979b2392ce5fa908af72021-12-02T14:26:47ZCalmodulin regulates the olfactory performance in Drosophila melanogaster10.1038/s41598-021-83296-92045-2322https://doaj.org/article/3948fd547a284979b2392ce5fa908af72021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83296-9https://doaj.org/toc/2045-2322Abstract Insect odorant receptors (ORs) detect volatile chemical cues with high sensitivity. These ORs operate as ligand-gated ion channels and are formed by heptahelical OrX and Orco (co-receptor) proteins. A highly conserved calmodulin (CaM) binding site (CBS) 336SAIKYWVER344 within the second intracellular loop of Drosophila melanogaster Orco constitutes a target for regulating OR performance. Here we asked how a point mutation K339N in this CBS affects the olfactory performance of Drosophila melanogaster. We first asked how this mutation would affect the odor responses of olfactory sensory neurons (OSNs). Using Ca2+ imaging in an ex-vivo antenna preparation, we activated all OR (OrX/Orco) expressing neurons using the synthetic agonist VUAA1. In a next attempt, we restricted the OR spectrum to Or22a expressing neurons (Or22a/Orco) and stimulated these OSNs with the ligand ethyl hexanoate. In both approaches, we found that flies carrying the K339N point mutation in Orco display a reduced olfactory response. We also found that the mutation abolishes the capability of OSNs to sensitize by repeated weak odor stimuli. Next, we asked whether OrcoK339N might affect the odor localization performance. Using a wind tunnel bioassay, we found that odor localization in flies carrying the OrcoK339N mutation was severely diminished.Kalpana JainSofia Lavista-LlanosVeit GrabeBill S. HanssonDieter WicherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kalpana Jain Sofia Lavista-Llanos Veit Grabe Bill S. Hansson Dieter Wicher Calmodulin regulates the olfactory performance in Drosophila melanogaster
description	Abstract Insect odorant receptors (ORs) detect volatile chemical cues with high sensitivity. These ORs operate as ligand-gated ion channels and are formed by heptahelical OrX and Orco (co-receptor) proteins. A highly conserved calmodulin (CaM) binding site (CBS) 336SAIKYWVER344 within the second intracellular loop of Drosophila melanogaster Orco constitutes a target for regulating OR performance. Here we asked how a point mutation K339N in this CBS affects the olfactory performance of Drosophila melanogaster. We first asked how this mutation would affect the odor responses of olfactory sensory neurons (OSNs). Using Ca2+ imaging in an ex-vivo antenna preparation, we activated all OR (OrX/Orco) expressing neurons using the synthetic agonist VUAA1. In a next attempt, we restricted the OR spectrum to Or22a expressing neurons (Or22a/Orco) and stimulated these OSNs with the ligand ethyl hexanoate. In both approaches, we found that flies carrying the K339N point mutation in Orco display a reduced olfactory response. We also found that the mutation abolishes the capability of OSNs to sensitize by repeated weak odor stimuli. Next, we asked whether OrcoK339N might affect the odor localization performance. Using a wind tunnel bioassay, we found that odor localization in flies carrying the OrcoK339N mutation was severely diminished.
format	article
author	Kalpana Jain Sofia Lavista-Llanos Veit Grabe Bill S. Hansson Dieter Wicher
author_facet	Kalpana Jain Sofia Lavista-Llanos Veit Grabe Bill S. Hansson Dieter Wicher
author_sort	Kalpana Jain
title	Calmodulin regulates the olfactory performance in Drosophila melanogaster
title_short	Calmodulin regulates the olfactory performance in Drosophila melanogaster
title_full	Calmodulin regulates the olfactory performance in Drosophila melanogaster
title_fullStr	Calmodulin regulates the olfactory performance in Drosophila melanogaster
title_full_unstemmed	Calmodulin regulates the olfactory performance in Drosophila melanogaster
title_sort	calmodulin regulates the olfactory performance in drosophila melanogaster
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3948fd547a284979b2392ce5fa908af7
work_keys_str_mv	AT kalpanajain calmodulinregulatestheolfactoryperformanceindrosophilamelanogaster AT sofialavistallanos calmodulinregulatestheolfactoryperformanceindrosophilamelanogaster AT veitgrabe calmodulinregulatestheolfactoryperformanceindrosophilamelanogaster AT billshansson calmodulinregulatestheolfactoryperformanceindrosophilamelanogaster AT dieterwicher calmodulinregulatestheolfactoryperformanceindrosophilamelanogaster
_version_	1718391304110473216

Calmodulin regulates the olfactory performance in Drosophila melanogaster

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