Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.

Microbial colonization of plant roots is a highly complex process that requires the coordination and regulation of many gene networks, yet the identities and functions of many of these gene products have yet to be discovered. Pantoea sp. YR343, a gamma-proteobacterium isolated from the rhizosphere o...

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Autores principales:	Amber N Bible, Mang Chang, Jennifer L Morrell-Falvey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/394d0a2a25d04cfa8163fa176bdb011b
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spelling	oai:doaj.org-article:394d0a2a25d04cfa8163fa176bdb011b2021-12-02T20:04:59ZIdentification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.1932-620310.1371/journal.pone.0248607https://doaj.org/article/394d0a2a25d04cfa8163fa176bdb011b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0248607https://doaj.org/toc/1932-6203Microbial colonization of plant roots is a highly complex process that requires the coordination and regulation of many gene networks, yet the identities and functions of many of these gene products have yet to be discovered. Pantoea sp. YR343, a gamma-proteobacterium isolated from the rhizosphere of Populus deltoides, forms robust biofilms along the root surfaces of Populus and possesses plant growth-promoting characteristics. In this work, we identified three diguanylate cyclases in the plant-associated microbe Pantoea sp. YR343 that are expressed in the presence of plant roots. One of these diguanylate cyclases, DGC2884, localizes to discrete sites in the cells and its overexpression results in reduced motility and increased EPS production and biofilm formation. We performed a genetic screen by expressing this diguanylate cyclase from an inducible promoter in order to identify candidate gene products that may be involved in root colonization by Pantoea sp. YR343. Further, we demonstrate the importance of other domains in DGC2884 to its activity, which in combination with the genes identified by transposon mutagenesis, may yield insights into the mechanisms of plant association as well as the activity and regulation of homologous enzymes in medically and agriculturally relevant microbes.Amber N BibleMang ChangJennifer L Morrell-FalveyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0248607 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amber N Bible Mang Chang Jennifer L Morrell-Falvey Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.
description	Microbial colonization of plant roots is a highly complex process that requires the coordination and regulation of many gene networks, yet the identities and functions of many of these gene products have yet to be discovered. Pantoea sp. YR343, a gamma-proteobacterium isolated from the rhizosphere of Populus deltoides, forms robust biofilms along the root surfaces of Populus and possesses plant growth-promoting characteristics. In this work, we identified three diguanylate cyclases in the plant-associated microbe Pantoea sp. YR343 that are expressed in the presence of plant roots. One of these diguanylate cyclases, DGC2884, localizes to discrete sites in the cells and its overexpression results in reduced motility and increased EPS production and biofilm formation. We performed a genetic screen by expressing this diguanylate cyclase from an inducible promoter in order to identify candidate gene products that may be involved in root colonization by Pantoea sp. YR343. Further, we demonstrate the importance of other domains in DGC2884 to its activity, which in combination with the genes identified by transposon mutagenesis, may yield insights into the mechanisms of plant association as well as the activity and regulation of homologous enzymes in medically and agriculturally relevant microbes.
format	article
author	Amber N Bible Mang Chang Jennifer L Morrell-Falvey
author_facet	Amber N Bible Mang Chang Jennifer L Morrell-Falvey
author_sort	Amber N Bible
title	Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.
title_short	Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.
title_full	Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.
title_fullStr	Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.
title_full_unstemmed	Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.
title_sort	identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by pantoea sp. yr343.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/394d0a2a25d04cfa8163fa176bdb011b
work_keys_str_mv	AT ambernbible identificationofadiguanylatecyclaseexpressedinthepresenceofplantsanditsapplicationfordiscoveringcandidategeneproductsinvolvedinplantcolonizationbypantoeaspyr343 AT mangchang identificationofadiguanylatecyclaseexpressedinthepresenceofplantsanditsapplicationfordiscoveringcandidategeneproductsinvolvedinplantcolonizationbypantoeaspyr343 AT jenniferlmorrellfalvey identificationofadiguanylatecyclaseexpressedinthepresenceofplantsanditsapplicationfordiscoveringcandidategeneproductsinvolvedinplantcolonizationbypantoeaspyr343
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Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343.

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