Flow Cytometry: A Blessing and a Curse

Flow cytometry is a laser-based technology generating a scattered and a fluorescent light signal that enables rapid analysis of the size and granularity of a particle or single cell. In addition, it offers the opportunity to phenotypically characterize and collect the cell with the use of a variety...

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Autores principales:	Hannah Drescher, Sabine Weiskirchen, Ralf Weiskirchen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	flow cytometry multiparameter analysis immunophenotyping Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/39528bdbf9f1489db0c3ef99247753dc
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spelling	oai:doaj.org-article:39528bdbf9f1489db0c3ef99247753dc2021-11-25T16:49:37ZFlow Cytometry: A Blessing and a Curse10.3390/biomedicines91116132227-9059https://doaj.org/article/39528bdbf9f1489db0c3ef99247753dc2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1613https://doaj.org/toc/2227-9059Flow cytometry is a laser-based technology generating a scattered and a fluorescent light signal that enables rapid analysis of the size and granularity of a particle or single cell. In addition, it offers the opportunity to phenotypically characterize and collect the cell with the use of a variety of fluorescent reagents. These reagents include but are not limited to fluorochrome-conjugated antibodies, fluorescent expressing protein-, viability-, and DNA-binding dyes. Major developments in reagents, electronics, and software within the last 30 years have greatly expanded the ability to combine up to 50 antibodies in one single tube. However, these advances also harbor technical risks and interpretation issues in the identification of certain cell populations which will be summarized in this viewpoint article. It will further provide an overview of different potential applications of flow cytometry in research and its possibilities to be used in the clinic.Hannah DrescherSabine WeiskirchenRalf WeiskirchenMDPI AGarticleflow cytometrymultiparameter analysisimmunophenotypingBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1613, p 1613 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	flow cytometry multiparameter analysis immunophenotyping Biology (General) QH301-705.5
spellingShingle	flow cytometry multiparameter analysis immunophenotyping Biology (General) QH301-705.5 Hannah Drescher Sabine Weiskirchen Ralf Weiskirchen Flow Cytometry: A Blessing and a Curse
description	Flow cytometry is a laser-based technology generating a scattered and a fluorescent light signal that enables rapid analysis of the size and granularity of a particle or single cell. In addition, it offers the opportunity to phenotypically characterize and collect the cell with the use of a variety of fluorescent reagents. These reagents include but are not limited to fluorochrome-conjugated antibodies, fluorescent expressing protein-, viability-, and DNA-binding dyes. Major developments in reagents, electronics, and software within the last 30 years have greatly expanded the ability to combine up to 50 antibodies in one single tube. However, these advances also harbor technical risks and interpretation issues in the identification of certain cell populations which will be summarized in this viewpoint article. It will further provide an overview of different potential applications of flow cytometry in research and its possibilities to be used in the clinic.
format	article
author	Hannah Drescher Sabine Weiskirchen Ralf Weiskirchen
author_facet	Hannah Drescher Sabine Weiskirchen Ralf Weiskirchen
author_sort	Hannah Drescher
title	Flow Cytometry: A Blessing and a Curse
title_short	Flow Cytometry: A Blessing and a Curse
title_full	Flow Cytometry: A Blessing and a Curse
title_fullStr	Flow Cytometry: A Blessing and a Curse
title_full_unstemmed	Flow Cytometry: A Blessing and a Curse
title_sort	flow cytometry: a blessing and a curse
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/39528bdbf9f1489db0c3ef99247753dc
work_keys_str_mv	AT hannahdrescher flowcytometryablessingandacurse AT sabineweiskirchen flowcytometryablessingandacurse AT ralfweiskirchen flowcytometryablessingandacurse
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Flow Cytometry: A Blessing and a Curse

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