Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota

Abstract Polydextrose (PDX) is a branched glucose polymer, utilized as a soluble dietary fiber. Recently, PDX was found to have hypolipidemic effects and effects on the gut microbiota. To investigate these findings more closely, a non-targeted metabolomics approach, was exploited to determine metabo...

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Autores principales:	Markku Tapani Saarinen, Olli Kärkkäinen, Kati Hanhineva, Kirsti Tiihonen, Ashley Hibberd, Kari Antero Mäkelä, Ghulam Shere Raza, Karl-Heinz Herzig, Heli Anglenius
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/395ad65f1db341a995befa71c59fc5a3
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spelling	oai:doaj.org-article:395ad65f1db341a995befa71c59fc5a32021-12-02T15:11:53ZMetabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota10.1038/s41598-020-78484-y2045-2322https://doaj.org/article/395ad65f1db341a995befa71c59fc5a32020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78484-yhttps://doaj.org/toc/2045-2322Abstract Polydextrose (PDX) is a branched glucose polymer, utilized as a soluble dietary fiber. Recently, PDX was found to have hypolipidemic effects and effects on the gut microbiota. To investigate these findings more closely, a non-targeted metabolomics approach, was exploited to determine metabolic alterations in blood and epididymal adipose tissue samples that were collected from C57BL/6 mice fed with a Western diet, with or without oral administration of PDX. Metabolomic analyses revealed significant differences between PDX- and control mice, which could be due to differences in diet or due to altered microbial metabolism in the gut. Some metabolites were found in both plasma and adipose tissue, such as the bile acid derivative deoxycholic acid and the microbiome-derived tryptophan metabolite indoxyl sulfate, both of which increased by PDX. Additionally, PDX increased the levels of glycine betaine and l-carnitine in plasma samples, which correlated negatively with plasma TG and positively correlated with bacterial genera enriched in PDX mice. The results demonstrated that PDX caused differential metabolite patterns in blood and adipose tissues and that one-carbon metabolism, associated with glycine betaine and l-carnitine, and bile acid and tryptophan metabolism are associated with the hypolipidemic effects observed in mice that were given PDX.Markku Tapani SaarinenOlli KärkkäinenKati HanhinevaKirsti TiihonenAshley HibberdKari Antero MäkeläGhulam Shere RazaKarl-Heinz HerzigHeli AngleniusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Markku Tapani Saarinen Olli Kärkkäinen Kati Hanhineva Kirsti Tiihonen Ashley Hibberd Kari Antero Mäkelä Ghulam Shere Raza Karl-Heinz Herzig Heli Anglenius Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota
description	Abstract Polydextrose (PDX) is a branched glucose polymer, utilized as a soluble dietary fiber. Recently, PDX was found to have hypolipidemic effects and effects on the gut microbiota. To investigate these findings more closely, a non-targeted metabolomics approach, was exploited to determine metabolic alterations in blood and epididymal adipose tissue samples that were collected from C57BL/6 mice fed with a Western diet, with or without oral administration of PDX. Metabolomic analyses revealed significant differences between PDX- and control mice, which could be due to differences in diet or due to altered microbial metabolism in the gut. Some metabolites were found in both plasma and adipose tissue, such as the bile acid derivative deoxycholic acid and the microbiome-derived tryptophan metabolite indoxyl sulfate, both of which increased by PDX. Additionally, PDX increased the levels of glycine betaine and l-carnitine in plasma samples, which correlated negatively with plasma TG and positively correlated with bacterial genera enriched in PDX mice. The results demonstrated that PDX caused differential metabolite patterns in blood and adipose tissues and that one-carbon metabolism, associated with glycine betaine and l-carnitine, and bile acid and tryptophan metabolism are associated with the hypolipidemic effects observed in mice that were given PDX.
format	article
author	Markku Tapani Saarinen Olli Kärkkäinen Kati Hanhineva Kirsti Tiihonen Ashley Hibberd Kari Antero Mäkelä Ghulam Shere Raza Karl-Heinz Herzig Heli Anglenius
author_facet	Markku Tapani Saarinen Olli Kärkkäinen Kati Hanhineva Kirsti Tiihonen Ashley Hibberd Kari Antero Mäkelä Ghulam Shere Raza Karl-Heinz Herzig Heli Anglenius
author_sort	Markku Tapani Saarinen
title	Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota
title_short	Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota
title_full	Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota
title_fullStr	Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota
title_full_unstemmed	Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota
title_sort	metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/395ad65f1db341a995befa71c59fc5a3
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Metabolomics analysis of plasma and adipose tissue samples from mice orally administered with polydextrose and correlations with cecal microbiota

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