Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis

The vertebrate eye primordium consists of a pseudostratified neuroepithelium, the optic vesicle (OV), in which cells acquire neural retina or retinal pigment epithelium (RPE) fates. As these fates arise, the OV assumes a cup shape, influenced by mechanical forces generated within the neural retina....

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Autores principales:	Tania Moreno-Mármol, Mario Ledesma-Terrón, Noemi Tabanera, Maria Jesús Martin-Bermejo, Marcos J Cardozo, Florencia Cavodeassi, Paola Bovolenta
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Zebrafish chick mouse human medaka optic cup Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3968577713244d2484f171fcf9df66c0
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spelling	oai:doaj.org-article:3968577713244d2484f171fcf9df66c02021-11-25T14:36:37ZStretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis10.7554/eLife.633962050-084Xe63396https://doaj.org/article/3968577713244d2484f171fcf9df66c02021-09-01T00:00:00Zhttps://elifesciences.org/articles/63396https://doaj.org/toc/2050-084XThe vertebrate eye primordium consists of a pseudostratified neuroepithelium, the optic vesicle (OV), in which cells acquire neural retina or retinal pigment epithelium (RPE) fates. As these fates arise, the OV assumes a cup shape, influenced by mechanical forces generated within the neural retina. Whether the RPE passively adapts to retinal changes or actively contributes to OV morphogenesis remains unexplored. We generated a zebrafish Tg(E1-bhlhe40:GFP) line to track RPE morphogenesis and interrogate its participation in OV folding. We show that, in virtual absence of proliferation, RPE cells stretch and flatten, thereby matching the retinal curvature and promoting OV folding. Localized interference with the RPE cytoskeleton disrupts tissue stretching and OV folding. Thus, extreme RPE flattening and accelerated differentiation are efficient solutions adopted by fast-developing species to enable timely optic cup formation. This mechanism differs in amniotes, in which proliferation drives RPE expansion with a much-reduced need of cell flattening.Tania Moreno-MármolMario Ledesma-TerrónNoemi TabaneraMaria Jesús Martin-BermejoMarcos J CardozoFlorencia CavodeassiPaola BovolentaeLife Sciences Publications LtdarticleZebrafishchickmousehumanmedakaoptic cupMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zebrafish chick mouse human medaka optic cup Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Zebrafish chick mouse human medaka optic cup Medicine R Science Q Biology (General) QH301-705.5 Tania Moreno-Mármol Mario Ledesma-Terrón Noemi Tabanera Maria Jesús Martin-Bermejo Marcos J Cardozo Florencia Cavodeassi Paola Bovolenta Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
description	The vertebrate eye primordium consists of a pseudostratified neuroepithelium, the optic vesicle (OV), in which cells acquire neural retina or retinal pigment epithelium (RPE) fates. As these fates arise, the OV assumes a cup shape, influenced by mechanical forces generated within the neural retina. Whether the RPE passively adapts to retinal changes or actively contributes to OV morphogenesis remains unexplored. We generated a zebrafish Tg(E1-bhlhe40:GFP) line to track RPE morphogenesis and interrogate its participation in OV folding. We show that, in virtual absence of proliferation, RPE cells stretch and flatten, thereby matching the retinal curvature and promoting OV folding. Localized interference with the RPE cytoskeleton disrupts tissue stretching and OV folding. Thus, extreme RPE flattening and accelerated differentiation are efficient solutions adopted by fast-developing species to enable timely optic cup formation. This mechanism differs in amniotes, in which proliferation drives RPE expansion with a much-reduced need of cell flattening.
format	article
author	Tania Moreno-Mármol Mario Ledesma-Terrón Noemi Tabanera Maria Jesús Martin-Bermejo Marcos J Cardozo Florencia Cavodeassi Paola Bovolenta
author_facet	Tania Moreno-Mármol Mario Ledesma-Terrón Noemi Tabanera Maria Jesús Martin-Bermejo Marcos J Cardozo Florencia Cavodeassi Paola Bovolenta
author_sort	Tania Moreno-Mármol
title	Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
title_short	Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
title_full	Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
title_fullStr	Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
title_full_unstemmed	Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
title_sort	stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/3968577713244d2484f171fcf9df66c0
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Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis

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