Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.

Colorectal neoplasia is the third most common cancer worldwide. Environmental factors such as diet are known to be involved in the etiology of this cancer. Several epidemiological studies have suggested that specific neo-formed mutagenic compounds related to meat consumption are an underlying factor...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Emilien L Jamin, Anne Riu, Thierry Douki, Laurent Debrauwer, Jean-Pierre Cravedi, Daniel Zalko, Marc Audebert
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
Materias:
R
Q
Acceso en línea:https://doaj.org/article/39771d298f9e4fc98816af087586c468
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:39771d298f9e4fc98816af087586c468
record_format dspace
spelling oai:doaj.org-article:39771d298f9e4fc98816af087586c4682021-11-18T07:54:31ZCombined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.1932-620310.1371/journal.pone.0058591https://doaj.org/article/39771d298f9e4fc98816af087586c4682013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23484039/?tool=EBIhttps://doaj.org/toc/1932-6203Colorectal neoplasia is the third most common cancer worldwide. Environmental factors such as diet are known to be involved in the etiology of this cancer. Several epidemiological studies have suggested that specific neo-formed mutagenic compounds related to meat consumption are an underlying factor involved in the association between diet and colorectal cancer. Heterocyclic amines (HCAs) and polycyclic aromatic hydrocarbons (PAHs) are known mutagens and possible human carcinogens formed at the same time in meat during cooking processes. We studied the genotoxicity of the model PAH benzo(a)pyrene (B(a)P) and HCA 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), alone or in mixture, using the mouse intestinal cell line Apc(Min/+), mimicking the early step of colorectal carcinogenesis, and control Apc(+/+) cells. The genotoxicity of B(a)P and PhIP was investigated using both cell lines, through the quantification of B(a)P and PhIP derived DNA adducts, as well as the use of a genotoxic assay based on histone H2AX phosphorylation quantification. Our results demonstrate that heterozygous Apc mutated cells are more effective to metabolize B(a)P. We also established in different experiments that PhIP and B(a)P were more genotoxic on Apc (Min/+) cells compared to Apc (+/+) . Moreover when tested in mixture, we observed a combined genotoxicity of B(a)P and PhIP on the two cell lines, with an increase of PhIP derived DNA adducts in the presence of B(a)P. Because of their genotoxic effects observed on heterozygous Apc mutated cells and their possible combined genotoxic effects, both B(a)P and PhIP, taken together, could be implicated in the observed association between meat consumption and colorectal cancer.Emilien L JaminAnne RiuThierry DoukiLaurent DebrauwerJean-Pierre CravediDaniel ZalkoMarc AudebertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58591 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Emilien L Jamin
Anne Riu
Thierry Douki
Laurent Debrauwer
Jean-Pierre Cravedi
Daniel Zalko
Marc Audebert
Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.
description Colorectal neoplasia is the third most common cancer worldwide. Environmental factors such as diet are known to be involved in the etiology of this cancer. Several epidemiological studies have suggested that specific neo-formed mutagenic compounds related to meat consumption are an underlying factor involved in the association between diet and colorectal cancer. Heterocyclic amines (HCAs) and polycyclic aromatic hydrocarbons (PAHs) are known mutagens and possible human carcinogens formed at the same time in meat during cooking processes. We studied the genotoxicity of the model PAH benzo(a)pyrene (B(a)P) and HCA 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), alone or in mixture, using the mouse intestinal cell line Apc(Min/+), mimicking the early step of colorectal carcinogenesis, and control Apc(+/+) cells. The genotoxicity of B(a)P and PhIP was investigated using both cell lines, through the quantification of B(a)P and PhIP derived DNA adducts, as well as the use of a genotoxic assay based on histone H2AX phosphorylation quantification. Our results demonstrate that heterozygous Apc mutated cells are more effective to metabolize B(a)P. We also established in different experiments that PhIP and B(a)P were more genotoxic on Apc (Min/+) cells compared to Apc (+/+) . Moreover when tested in mixture, we observed a combined genotoxicity of B(a)P and PhIP on the two cell lines, with an increase of PhIP derived DNA adducts in the presence of B(a)P. Because of their genotoxic effects observed on heterozygous Apc mutated cells and their possible combined genotoxic effects, both B(a)P and PhIP, taken together, could be implicated in the observed association between meat consumption and colorectal cancer.
format article
author Emilien L Jamin
Anne Riu
Thierry Douki
Laurent Debrauwer
Jean-Pierre Cravedi
Daniel Zalko
Marc Audebert
author_facet Emilien L Jamin
Anne Riu
Thierry Douki
Laurent Debrauwer
Jean-Pierre Cravedi
Daniel Zalko
Marc Audebert
author_sort Emilien L Jamin
title Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.
title_short Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.
title_full Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.
title_fullStr Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.
title_full_unstemmed Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.
title_sort combined genotoxic effects of a polycyclic aromatic hydrocarbon (b(a)p) and an heterocyclic amine (phip) in relation to colorectal carcinogenesis.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/39771d298f9e4fc98816af087586c468
work_keys_str_mv AT emilienljamin combinedgenotoxiceffectsofapolycyclicaromatichydrocarbonbapandanheterocyclicaminephipinrelationtocolorectalcarcinogenesis
AT anneriu combinedgenotoxiceffectsofapolycyclicaromatichydrocarbonbapandanheterocyclicaminephipinrelationtocolorectalcarcinogenesis
AT thierrydouki combinedgenotoxiceffectsofapolycyclicaromatichydrocarbonbapandanheterocyclicaminephipinrelationtocolorectalcarcinogenesis
AT laurentdebrauwer combinedgenotoxiceffectsofapolycyclicaromatichydrocarbonbapandanheterocyclicaminephipinrelationtocolorectalcarcinogenesis
AT jeanpierrecravedi combinedgenotoxiceffectsofapolycyclicaromatichydrocarbonbapandanheterocyclicaminephipinrelationtocolorectalcarcinogenesis
AT danielzalko combinedgenotoxiceffectsofapolycyclicaromatichydrocarbonbapandanheterocyclicaminephipinrelationtocolorectalcarcinogenesis
AT marcaudebert combinedgenotoxiceffectsofapolycyclicaromatichydrocarbonbapandanheterocyclicaminephipinrelationtocolorectalcarcinogenesis
_version_ 1718422778518962176