Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis

Kingella kingae is an emerging pathogen that causes septic arthritis, osteomyelitis, and bacteremia in children from 6 to 48 months of age. The presence of bacteria within or near the bone is associated with an inflammatory process that results in osteolysis, but the underlying pathogenic mechanisms...

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Autores principales:	Ayelén Ivana Pesce Viglietti, Franco Agustín Sviercz, Cinthya Alicia Marcela López, Rosa Nicole Freiberger, Jorge Quarleri, María Victoria Delpino
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	inflammation osteoclastogenesis and bone loss Kingella kingae TNF-α IL-1β Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/397eb69daa5f4501b547431ffa93d03d
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id	oai:doaj.org-article:397eb69daa5f4501b547431ffa93d03d
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spelling	oai:doaj.org-article:397eb69daa5f4501b547431ffa93d03d2021-12-02T10:35:51ZProinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis1664-322410.3389/fimmu.2021.757827https://doaj.org/article/397eb69daa5f4501b547431ffa93d03d2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.757827/fullhttps://doaj.org/toc/1664-3224Kingella kingae is an emerging pathogen that causes septic arthritis, osteomyelitis, and bacteremia in children from 6 to 48 months of age. The presence of bacteria within or near the bone is associated with an inflammatory process that results in osteolysis, but the underlying pathogenic mechanisms involved are largely unknown. To determine the link between K. kingae and bone loss, we have assessed whether infection per se or through the genesis of a pro-inflammatory microenvironment can promote osteoclastogenesis. For that purpose, we examined both the direct effect of K. kingae and the immune-mediated mechanism involved in K. kingae-infected macrophage-induced osteoclastogenesis. Our results indicate that osteoclastogenesis is stimulated by K. kingae infection directly and indirectly by fueling a potent pro-inflammatory response that drives macrophages to undergo functional osteoclasts via TNF-α and IL-1β induction. Such osteoclastogenic capability of K. kingae is counteracted by their outer membrane vesicles (OMV) in a concentration-dependent manner. In conclusion, this model allowed elucidating the interplay between the K. kingae and their OMV to modulate osteoclastogenesis from exposed macrophages, thus contributing to the modulation in joint and bone damage.Ayelén Ivana Pesce VigliettiFranco Agustín SvierczCinthya Alicia Marcela LópezRosa Nicole FreibergerJorge QuarleriMaría Victoria DelpinoFrontiers Media S.A.articleinflammationosteoclastogenesis and bone lossKingella kingaeTNF-αIL-1βImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammation osteoclastogenesis and bone loss Kingella kingae TNF-α IL-1β Immunologic diseases. Allergy RC581-607
spellingShingle	inflammation osteoclastogenesis and bone loss Kingella kingae TNF-α IL-1β Immunologic diseases. Allergy RC581-607 Ayelén Ivana Pesce Viglietti Franco Agustín Sviercz Cinthya Alicia Marcela López Rosa Nicole Freiberger Jorge Quarleri María Victoria Delpino Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis
description	Kingella kingae is an emerging pathogen that causes septic arthritis, osteomyelitis, and bacteremia in children from 6 to 48 months of age. The presence of bacteria within or near the bone is associated with an inflammatory process that results in osteolysis, but the underlying pathogenic mechanisms involved are largely unknown. To determine the link between K. kingae and bone loss, we have assessed whether infection per se or through the genesis of a pro-inflammatory microenvironment can promote osteoclastogenesis. For that purpose, we examined both the direct effect of K. kingae and the immune-mediated mechanism involved in K. kingae-infected macrophage-induced osteoclastogenesis. Our results indicate that osteoclastogenesis is stimulated by K. kingae infection directly and indirectly by fueling a potent pro-inflammatory response that drives macrophages to undergo functional osteoclasts via TNF-α and IL-1β induction. Such osteoclastogenic capability of K. kingae is counteracted by their outer membrane vesicles (OMV) in a concentration-dependent manner. In conclusion, this model allowed elucidating the interplay between the K. kingae and their OMV to modulate osteoclastogenesis from exposed macrophages, thus contributing to the modulation in joint and bone damage.
format	article
author	Ayelén Ivana Pesce Viglietti Franco Agustín Sviercz Cinthya Alicia Marcela López Rosa Nicole Freiberger Jorge Quarleri María Victoria Delpino
author_facet	Ayelén Ivana Pesce Viglietti Franco Agustín Sviercz Cinthya Alicia Marcela López Rosa Nicole Freiberger Jorge Quarleri María Victoria Delpino
author_sort	Ayelén Ivana Pesce Viglietti
title	Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis
title_short	Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis
title_full	Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis
title_fullStr	Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis
title_full_unstemmed	Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis
title_sort	proinflammatory microenvironment during kingella kingae infection modulates osteoclastogenesis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/397eb69daa5f4501b547431ffa93d03d
work_keys_str_mv	AT ayelenivanapesceviglietti proinflammatorymicroenvironmentduringkingellakingaeinfectionmodulatesosteoclastogenesis AT francoagustinsviercz proinflammatorymicroenvironmentduringkingellakingaeinfectionmodulatesosteoclastogenesis AT cinthyaaliciamarcelalopez proinflammatorymicroenvironmentduringkingellakingaeinfectionmodulatesosteoclastogenesis AT rosanicolefreiberger proinflammatorymicroenvironmentduringkingellakingaeinfectionmodulatesosteoclastogenesis AT jorgequarleri proinflammatorymicroenvironmentduringkingellakingaeinfectionmodulatesosteoclastogenesis AT mariavictoriadelpino proinflammatorymicroenvironmentduringkingellakingaeinfectionmodulatesosteoclastogenesis
_version_	1718396926152409088

Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis

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