Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.

Cell-derived microparticles participate in intercellular communication similar to the classical messenger systems of small and macro-molecules that bind to specialized membrane receptors. Microparticles have been implicated in the regulation of a variety of complex physiopathologic processes, such a...

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Autores principales:	Panpan Chen, Steven D Douglas, John Meshki, Florin Tuluc
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3980cfad27654023ae221c52b688c769
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spelling	oai:doaj.org-article:3980cfad27654023ae221c52b688c7692021-11-18T07:05:27ZNeurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.1932-620310.1371/journal.pone.0045322https://doaj.org/article/3980cfad27654023ae221c52b688c7692012-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0045322https://doaj.org/toc/1932-6203Cell-derived microparticles participate in intercellular communication similar to the classical messenger systems of small and macro-molecules that bind to specialized membrane receptors. Microparticles have been implicated in the regulation of a variety of complex physiopathologic processes, such as thrombosis, the control of innate and adaptive immunity, and cancer. The neurokinin 1 receptor (NK1R) is a Gq-coupled receptor present on the membrane of a variety of tissues, including neurons in the central and peripheral nervous system, immune cells, endocrine and exocrine glands, and smooth muscle. The endogenous agonist of NK1R is the undecapeptide substance P (SP). We have previously described intracellular signaling mechanisms that regulate NK1R-mediated rapid cell shape changes in HEK293 cells and U373MG cells. In the present study, we show that the activation of NK1R in HEK293 cells, but not in U373MG cells, leads to formation of sheer-stress induced microparticles that stain positive with the membrane-selective fluorescent dye FM 2-10. SP-induced microparticle formation is independent of elevated intracellular calcium concentrations and activation of NK1R present on HEK293-derived microparticles triggers detectable calcium increase in SP-induced microparticles. The ROCK inhibitor Y27632 and the dynamin inhibitor dynasore inhibited membrane blebbing and microparticle formation in HEK293 cells, strongly suggesting that microparticle formation in this cell type is dependent on membrane blebbing.Panpan ChenSteven D DouglasJohn MeshkiFlorin TulucPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45322 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Panpan Chen Steven D Douglas John Meshki Florin Tuluc Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.
description	Cell-derived microparticles participate in intercellular communication similar to the classical messenger systems of small and macro-molecules that bind to specialized membrane receptors. Microparticles have been implicated in the regulation of a variety of complex physiopathologic processes, such as thrombosis, the control of innate and adaptive immunity, and cancer. The neurokinin 1 receptor (NK1R) is a Gq-coupled receptor present on the membrane of a variety of tissues, including neurons in the central and peripheral nervous system, immune cells, endocrine and exocrine glands, and smooth muscle. The endogenous agonist of NK1R is the undecapeptide substance P (SP). We have previously described intracellular signaling mechanisms that regulate NK1R-mediated rapid cell shape changes in HEK293 cells and U373MG cells. In the present study, we show that the activation of NK1R in HEK293 cells, but not in U373MG cells, leads to formation of sheer-stress induced microparticles that stain positive with the membrane-selective fluorescent dye FM 2-10. SP-induced microparticle formation is independent of elevated intracellular calcium concentrations and activation of NK1R present on HEK293-derived microparticles triggers detectable calcium increase in SP-induced microparticles. The ROCK inhibitor Y27632 and the dynamin inhibitor dynasore inhibited membrane blebbing and microparticle formation in HEK293 cells, strongly suggesting that microparticle formation in this cell type is dependent on membrane blebbing.
format	article
author	Panpan Chen Steven D Douglas John Meshki Florin Tuluc
author_facet	Panpan Chen Steven D Douglas John Meshki Florin Tuluc
author_sort	Panpan Chen
title	Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.
title_short	Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.
title_full	Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.
title_fullStr	Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.
title_full_unstemmed	Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.
title_sort	neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in hek293 cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/3980cfad27654023ae221c52b688c769
work_keys_str_mv	AT panpanchen neurokinin1receptormediatesmembraneblebbingandsheerstressinducedmicroparticleformationinhek293cells AT stevenddouglas neurokinin1receptormediatesmembraneblebbingandsheerstressinducedmicroparticleformationinhek293cells AT johnmeshki neurokinin1receptormediatesmembraneblebbingandsheerstressinducedmicroparticleformationinhek293cells AT florintuluc neurokinin1receptormediatesmembraneblebbingandsheerstressinducedmicroparticleformationinhek293cells
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Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.

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