The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Dar-Shih Hsieh1,2, Hsiu-Chin Lu5, Cheng-Cheung Chen1,3, Chang-Jer Wu1,*, Ming-Kung Yeh4,* 1Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan; 2Division of Urology, 3Department of Pathology, Tri-Service General Hospital, 4Institute of Preventive Medicine, National Defence...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hsieh DS, Lu HC, Chen CC, Wu CJ, Yeh MK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/398b612a58d0441cb192bda7e2aca5f3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:398b612a58d0441cb192bda7e2aca5f3
record_format	dspace
spelling	oai:doaj.org-article:398b612a58d0441cb192bda7e2aca5f32021-12-02T02:49:04ZThe preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system1176-91141178-2013https://doaj.org/article/398b612a58d0441cb192bda7e2aca5f32012-03-01T00:00:00Zhttp://www.dovepress.com/the-preparation-and-characterization-of-gold-conjugated-polyphenol-nan-a9587https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Dar-Shih Hsieh1,2, Hsiu-Chin Lu5, Cheng-Cheung Chen1,3, Chang-Jer Wu1,, Ming-Kung Yeh4, 1Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan; 2Division of Urology, 3Department of Pathology, Tri-Service General Hospital, 4Institute of Preventive Medicine, National Defence Medical Center, Taipei, Taiwan; 5Department of Laboratory Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan*These authors contributed equally to this workAbstract: Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% &plusmn; 0.02%) to 23:5 (28% &plusmn; 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to &ndash;22 mV, respectively. At a ratio of 23:2.5, the EGCG-pNG particles (27% EGCG, 50 nm in size, zeta potential of &ndash;8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities.Keywords: EGCG, nanoparticles, antioxidant, antitumor activity, nanogoldHsieh DSLu HCChen CCWu CJYeh MKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1623-1633 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hsieh DS Lu HC Chen CC Wu CJ Yeh MK The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
description	Dar-Shih Hsieh1,2, Hsiu-Chin Lu5, Cheng-Cheung Chen1,3, Chang-Jer Wu1,, Ming-Kung Yeh4, 1Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan; 2Division of Urology, 3Department of Pathology, Tri-Service General Hospital, 4Institute of Preventive Medicine, National Defence Medical Center, Taipei, Taiwan; 5Department of Laboratory Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan*These authors contributed equally to this workAbstract: Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% &plusmn; 0.02%) to 23:5 (28% &plusmn; 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to &ndash;22 mV, respectively. At a ratio of 23:2.5, the EGCG-pNG particles (27% EGCG, 50 nm in size, zeta potential of &ndash;8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities.Keywords: EGCG, nanoparticles, antioxidant, antitumor activity, nanogold
format	article
author	Hsieh DS Lu HC Chen CC Wu CJ Yeh MK
author_facet	Hsieh DS Lu HC Chen CC Wu CJ Yeh MK
author_sort	Hsieh DS
title	The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_short	The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_full	The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_fullStr	The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_full_unstemmed	The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
title_sort	preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/398b612a58d0441cb192bda7e2aca5f3
work_keys_str_mv	AT hsiehds thepreparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT luhc thepreparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT chencc thepreparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT wucj thepreparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT yehmk thepreparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT hsiehds preparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT luhc preparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT chencc preparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT wucj preparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem AT yehmk preparationandcharacterizationofgoldconjugatedpolyphenolnanoparticlesasanoveldeliverysystem
_version_	1718402095234678784

The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Ejemplares similares