Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age

Abstract Piedmontese cattle is known for double-muscle phenotype. MicroRNAs (miRNAs) play important role as regulators in skeletal muscle physiological processes, and we hypothesize that plasma miRNAs expression profiles could be affected by skeletal muscle growth status related to age. Plasma sampl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rupal S. Tewari, Ugo Ala, Paolo Accornero, Mario Baratta, Silvia Miretti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/398d2c366e174e6b82a72b5304477e25
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:398d2c366e174e6b82a72b5304477e25
record_format	dspace
spelling	oai:doaj.org-article:398d2c366e174e6b82a72b5304477e252021-12-02T18:49:25ZCirculating skeletal muscle related microRNAs profile in Piedmontese cattle during different age10.1038/s41598-021-95137-w2045-2322https://doaj.org/article/398d2c366e174e6b82a72b5304477e252021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95137-whttps://doaj.org/toc/2045-2322Abstract Piedmontese cattle is known for double-muscle phenotype. MicroRNAs (miRNAs) play important role as regulators in skeletal muscle physiological processes, and we hypothesize that plasma miRNAs expression profiles could be affected by skeletal muscle growth status related to age. Plasma samples of cattle were collected during four different ages from first week of life until the time of commercial end of the fattening period before slaughter. Small-RNA sequencing data analysis revealed the presence of 40% of muscle-related miRNAs among the top 25 highly expressed miRNAs and, 19 miRNAs showed differential expression too. Using qRT-PCR, we validated in a larger bovine population, miRNAs involved in skeletal muscle physiology pathways. Comparing new-born with the other age groups, miR-10b, miR-126-5p, miR-143 and miR-146b were significantly up-regulated, whereas miR-21-5p, miR-221, miR-223 and miR-30b-5p were significantly down-regulated. High expression levels of miR-23a in all the groups were found. Myostatin, a negative regulator of skeletal muscle hypertrophy, was predicted as the target gene for miR-23a and miR-126-5p and we demonstrated their direct binding. Correlation analysis revealed association between miRNAs expression profiles and animals’ weights along the age. Circulating miRNAs could be promising for future studies on their biomarker potentialities to beef cattle selection.Rupal S. TewariUgo AlaPaolo AccorneroMario BarattaSilvia MirettiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rupal S. Tewari Ugo Ala Paolo Accornero Mario Baratta Silvia Miretti Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age
description	Abstract Piedmontese cattle is known for double-muscle phenotype. MicroRNAs (miRNAs) play important role as regulators in skeletal muscle physiological processes, and we hypothesize that plasma miRNAs expression profiles could be affected by skeletal muscle growth status related to age. Plasma samples of cattle were collected during four different ages from first week of life until the time of commercial end of the fattening period before slaughter. Small-RNA sequencing data analysis revealed the presence of 40% of muscle-related miRNAs among the top 25 highly expressed miRNAs and, 19 miRNAs showed differential expression too. Using qRT-PCR, we validated in a larger bovine population, miRNAs involved in skeletal muscle physiology pathways. Comparing new-born with the other age groups, miR-10b, miR-126-5p, miR-143 and miR-146b were significantly up-regulated, whereas miR-21-5p, miR-221, miR-223 and miR-30b-5p were significantly down-regulated. High expression levels of miR-23a in all the groups were found. Myostatin, a negative regulator of skeletal muscle hypertrophy, was predicted as the target gene for miR-23a and miR-126-5p and we demonstrated their direct binding. Correlation analysis revealed association between miRNAs expression profiles and animals’ weights along the age. Circulating miRNAs could be promising for future studies on their biomarker potentialities to beef cattle selection.
format	article
author	Rupal S. Tewari Ugo Ala Paolo Accornero Mario Baratta Silvia Miretti
author_facet	Rupal S. Tewari Ugo Ala Paolo Accornero Mario Baratta Silvia Miretti
author_sort	Rupal S. Tewari
title	Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age
title_short	Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age
title_full	Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age
title_fullStr	Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age
title_full_unstemmed	Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age
title_sort	circulating skeletal muscle related micrornas profile in piedmontese cattle during different age
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/398d2c366e174e6b82a72b5304477e25
work_keys_str_mv	AT rupalstewari circulatingskeletalmusclerelatedmicrornasprofileinpiedmontesecattleduringdifferentage AT ugoala circulatingskeletalmusclerelatedmicrornasprofileinpiedmontesecattleduringdifferentage AT paoloaccornero circulatingskeletalmusclerelatedmicrornasprofileinpiedmontesecattleduringdifferentage AT mariobaratta circulatingskeletalmusclerelatedmicrornasprofileinpiedmontesecattleduringdifferentage AT silviamiretti circulatingskeletalmusclerelatedmicrornasprofileinpiedmontesecattleduringdifferentage
_version_	1718377555032014848

Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age

Ejemplares similares