microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2

Qian Liu,1 Yaguang Hu,2 Min Zhang,3 Yousheng Yan,4 Hongmei Yu,1 Li Ge1 1Department of Neurological Rehabilitation, Gansu Provincial Hospital, Gansu 730000, China; 2Department of Pharmacy, Gansu Provincial Maternity and Child-Care Hospital, Gansu 730050, China; 3Department of Pathology, Gansu Provin...

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Autores principales:	Liu Q, Hu Y, Zhang M, Yan Y, Yu H, Ge L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	miR-451 CELF2 I/R injury neuron oxidative stress apoptosis. Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/398d45559a6c4659ba28698c39b43144
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spelling	oai:doaj.org-article:398d45559a6c4659ba28698c39b431442021-12-02T07:55:46ZmicroRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF21178-2021https://doaj.org/article/398d45559a6c4659ba28698c39b431442018-10-01T00:00:00Zhttps://www.dovepress.com/microrna-451-protects-neurons-against-ischemiareperfusion-injury-induc-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Qian Liu,1 Yaguang Hu,2 Min Zhang,3 Yousheng Yan,4 Hongmei Yu,1 Li Ge1 1Department of Neurological Rehabilitation, Gansu Provincial Hospital, Gansu 730000, China; 2Department of Pharmacy, Gansu Provincial Maternity and Child-Care Hospital, Gansu 730050, China; 3Department of Pathology, Gansu Provincial Hospital, Gansu 730000, China; 4National Centre for Human Genetic Resources, National Research Institute for Health and Family Planning, Beijing 100089, China Objectives: miRNAs are a family of non-coding RNAs that affect cell growth, migration and apoptosis. However, little is known on the behavior of miRNAs in neurons. Hence, this work aimed to investigate the functions and roles of miRNA-451 in neurons induced by ischemia/reperfusion injury.Materials and methods: In this study, we established a 12- or 24-hour oxygen and glucose deprivation/reoxygenation (OGD/R) cell model. miR-451 mimic, si-CUGBP Elav-like family member 2 (siCELF2), oeCELF2 and the corresponding negative controls were transfected into the 24-hour OGD/R cells. The transfection efficiency and the relative expression of miR-451 and CELF2 were measured using quantitative reverse transcription PCR and Western blot analysis. Cell viability, apoptosis, oxidative stress and cleaved-caspase-3 expression were assessed using Cell Counting Kit-8, LDH, SOD, malondialdehyde, ROS assays, flow cytometry and Western blot analysis upon miR-451 overexpression, CELF2 silencing or overexpression of both. Bioinformatics analysis and the dual-luciferase reporter assay were used to examine the relationship between CELF2 and miR-451 in the OGD/R cells.Results: The results showed that miR-451 was downregulated in the OGD/R cells. The overexpression of miR-451 increased cell viability and SOD activity, but decreased apoptosis rate, levels of LDH, MDA, ROS and cleaved caspase-3 expression. CELF2 silencing inhibited apoptosis and oxidative stress. The results suggested that CELF2 was a target of miR-451, and that CELF2 overexpression alleviated the inhibitory effect of miR-451 on apoptosis and oxidative stress of the OGD/R cells.Conclusion: The results demonstrated that miR-451 could protect cells against OGD/R-induced apoptosis and oxidative stress by targeting CELF2. Keywords: miR-451, CELF2, I/R injury, neuron, oxidative stress, apoptosisLiu QHu YZhang MYan YYu HGe LDove Medical PressarticlemiR-451CELF2I/R injuryneuronoxidative stressapoptosis.Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 14, Pp 2773-2782 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-451 CELF2 I/R injury neuron oxidative stress apoptosis. Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	miR-451 CELF2 I/R injury neuron oxidative stress apoptosis. Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Liu Q Hu Y Zhang M Yan Y Yu H Ge L microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2
description	Qian Liu,1 Yaguang Hu,2 Min Zhang,3 Yousheng Yan,4 Hongmei Yu,1 Li Ge1 1Department of Neurological Rehabilitation, Gansu Provincial Hospital, Gansu 730000, China; 2Department of Pharmacy, Gansu Provincial Maternity and Child-Care Hospital, Gansu 730050, China; 3Department of Pathology, Gansu Provincial Hospital, Gansu 730000, China; 4National Centre for Human Genetic Resources, National Research Institute for Health and Family Planning, Beijing 100089, China Objectives: miRNAs are a family of non-coding RNAs that affect cell growth, migration and apoptosis. However, little is known on the behavior of miRNAs in neurons. Hence, this work aimed to investigate the functions and roles of miRNA-451 in neurons induced by ischemia/reperfusion injury.Materials and methods: In this study, we established a 12- or 24-hour oxygen and glucose deprivation/reoxygenation (OGD/R) cell model. miR-451 mimic, si-CUGBP Elav-like family member 2 (siCELF2), oeCELF2 and the corresponding negative controls were transfected into the 24-hour OGD/R cells. The transfection efficiency and the relative expression of miR-451 and CELF2 were measured using quantitative reverse transcription PCR and Western blot analysis. Cell viability, apoptosis, oxidative stress and cleaved-caspase-3 expression were assessed using Cell Counting Kit-8, LDH, SOD, malondialdehyde, ROS assays, flow cytometry and Western blot analysis upon miR-451 overexpression, CELF2 silencing or overexpression of both. Bioinformatics analysis and the dual-luciferase reporter assay were used to examine the relationship between CELF2 and miR-451 in the OGD/R cells.Results: The results showed that miR-451 was downregulated in the OGD/R cells. The overexpression of miR-451 increased cell viability and SOD activity, but decreased apoptosis rate, levels of LDH, MDA, ROS and cleaved caspase-3 expression. CELF2 silencing inhibited apoptosis and oxidative stress. The results suggested that CELF2 was a target of miR-451, and that CELF2 overexpression alleviated the inhibitory effect of miR-451 on apoptosis and oxidative stress of the OGD/R cells.Conclusion: The results demonstrated that miR-451 could protect cells against OGD/R-induced apoptosis and oxidative stress by targeting CELF2. Keywords: miR-451, CELF2, I/R injury, neuron, oxidative stress, apoptosis
format	article
author	Liu Q Hu Y Zhang M Yan Y Yu H Ge L
author_facet	Liu Q Hu Y Zhang M Yan Y Yu H Ge L
author_sort	Liu Q
title	microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2
title_short	microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2
title_full	microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2
title_fullStr	microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2
title_full_unstemmed	microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2
title_sort	microrna-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting celf2
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/398d45559a6c4659ba28698c39b43144
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microRNA-451 protects neurons against ischemia/reperfusion injury-induced cell death by targeting CELF2

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