Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender

Kacang goats can be bred through artificial insemination techniques using liquid semen from a superior buck. The study was aimed to determine the quality of Kacang buck semen in chilled storage when green tea extract (GTE) was added to the semen extender. Specifically, 12 ejaculates from three Kaca...

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Autores principales:	S. Susilowati, I. Mustofa, W. Wurlina, T. Hernawati, Y. Oktanella
Formato:	article
Lenguaje:	EN
Publicado:	IPB University 2021
Materias:	DNA fragmentation intact plasma membrane malondialdehyde sperm motility poverty reduction Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/398eee188a0e47a1bfb22e902a263a03
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spelling	oai:doaj.org-article:398eee188a0e47a1bfb22e902a263a032021-11-15T23:17:23ZMaintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender10.5398/tasj.2021.44.4.4082615-787X2615-790Xhttps://doaj.org/article/398eee188a0e47a1bfb22e902a263a032021-11-01T00:00:00Zhttps://journal.ipb.ac.id/index.php/tasj/article/view/32738https://doaj.org/toc/2615-787Xhttps://doaj.org/toc/2615-790X Kacang goats can be bred through artificial insemination techniques using liquid semen from a superior buck. The study was aimed to determine the quality of Kacang buck semen in chilled storage when green tea extract (GTE) was added to the semen extender. Specifically, 12 ejaculates from three Kacang bucks were diluted in skim milk–egg yolk extender containing 0 mg, 0.05, 0.10, and 0.15 mg of GTE/100 mL. The extended semen was stored at 5°C, and its quality was evaluated daily for 5 d. Comparatively, semen with 0.10 mg of GTE/100 mL had the best quality (p<0.05): it had the highest sperm viability, sperm progressive motility, and sperm intact plasma membrane values, and the lowest malondialdehyde levels and DNA fragmentation percentage. However, as the storage period increased, there was a decrease in sperm viability, sperm motility, and sperm intact plasma membrane values with an increase in malondialdehyde levels and DNA fragmentation (p<0.05). The sperm motility of semen with 0.10 mg of GTE/100 mL was maintained during 5 d of chilled storage to meet the qualification for artificial insemination. In contrast, the sperm motility of semen with 0.05 mg and 0.15 mg of GTE/100 mL was maintained for 4 d, whereas that of the control semen was maintained only for 3 d. Thus, the addition 0.10 mg of GTE/100 mL of skim milk-egg yolk extender seems to help maintain the quality of Kacang goat sperm in chilled storage. S. SusilowatiI. MustofaW. WurlinaT. HernawatiY. OktanellaIPB UniversityarticleDNA fragmentationintact plasma membranemalondialdehydesperm motilitypoverty reductionAnimal cultureSF1-1100ENTropical Animal Science Journal, Vol 44, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DNA fragmentation intact plasma membrane malondialdehyde sperm motility poverty reduction Animal culture SF1-1100
spellingShingle	DNA fragmentation intact plasma membrane malondialdehyde sperm motility poverty reduction Animal culture SF1-1100 S. Susilowati I. Mustofa W. Wurlina T. Hernawati Y. Oktanella Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender
description	Kacang goats can be bred through artificial insemination techniques using liquid semen from a superior buck. The study was aimed to determine the quality of Kacang buck semen in chilled storage when green tea extract (GTE) was added to the semen extender. Specifically, 12 ejaculates from three Kacang bucks were diluted in skim milk–egg yolk extender containing 0 mg, 0.05, 0.10, and 0.15 mg of GTE/100 mL. The extended semen was stored at 5°C, and its quality was evaluated daily for 5 d. Comparatively, semen with 0.10 mg of GTE/100 mL had the best quality (p<0.05): it had the highest sperm viability, sperm progressive motility, and sperm intact plasma membrane values, and the lowest malondialdehyde levels and DNA fragmentation percentage. However, as the storage period increased, there was a decrease in sperm viability, sperm motility, and sperm intact plasma membrane values with an increase in malondialdehyde levels and DNA fragmentation (p<0.05). The sperm motility of semen with 0.10 mg of GTE/100 mL was maintained during 5 d of chilled storage to meet the qualification for artificial insemination. In contrast, the sperm motility of semen with 0.05 mg and 0.15 mg of GTE/100 mL was maintained for 4 d, whereas that of the control semen was maintained only for 3 d. Thus, the addition 0.10 mg of GTE/100 mL of skim milk-egg yolk extender seems to help maintain the quality of Kacang goat sperm in chilled storage.
format	article
author	S. Susilowati I. Mustofa W. Wurlina T. Hernawati Y. Oktanella
author_facet	S. Susilowati I. Mustofa W. Wurlina T. Hernawati Y. Oktanella
author_sort	S. Susilowati
title	Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender
title_short	Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender
title_full	Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender
title_fullStr	Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender
title_full_unstemmed	Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender
title_sort	maintaining the quality of kacang buck semen in chilled storage with the addition of green tea extract in extender
publisher	IPB University
publishDate	2021
url	https://doaj.org/article/398eee188a0e47a1bfb22e902a263a03
work_keys_str_mv	AT ssusilowati maintainingthequalityofkacangbucksemeninchilledstoragewiththeadditionofgreenteaextractinextender AT imustofa maintainingthequalityofkacangbucksemeninchilledstoragewiththeadditionofgreenteaextractinextender AT wwurlina maintainingthequalityofkacangbucksemeninchilledstoragewiththeadditionofgreenteaextractinextender AT thernawati maintainingthequalityofkacangbucksemeninchilledstoragewiththeadditionofgreenteaextractinextender AT yoktanella maintainingthequalityofkacangbucksemeninchilledstoragewiththeadditionofgreenteaextractinextender
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Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender

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