Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course

The conversion of biomass to biofuels presents a solution to one of the largest global challenges of our era, climate change. A critical part of this pipeline is the process of breaking down cellulosic sugars from plant matter to be used by microbes containing biosynthetic pathways that produce biof...

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Autores principales:	Jesus F. Barajas, Maren Wehrs, Milton To, Lauchlin Cruickshanks, Rochelle Urban, Adrienne McKee, John Gladden, Ee-Been Goh, Margaret E. Brown, Diane Pierotti, James M. Carothers, Aindrila Mukhopadhyay, Jay D. Keasling, Jeffrey L. Fortman, Steven W. Singer, Constance B. Bailey
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/398f5e54588c411ca5f1dbbd33d8bc7a
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spelling	oai:doaj.org-article:398f5e54588c411ca5f1dbbd33d8bc7a2021-11-15T15:04:28ZIsolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course10.1128/jmbe.v20i2.17231935-78851935-7877https://doaj.org/article/398f5e54588c411ca5f1dbbd33d8bc7a2019-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v20i2.1723https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885The conversion of biomass to biofuels presents a solution to one of the largest global challenges of our era, climate change. A critical part of this pipeline is the process of breaking down cellulosic sugars from plant matter to be used by microbes containing biosynthetic pathways that produce biofuels or bioproducts. In this inquiry-based course, students complete a research project that isolates cellulase-producing bacteria from samples collected from the environment. After obtaining isolates, the students characterize the production of cellulases. Students then amplify and sequence the 16S rRNA genes of confirmed cellulase producers and use bioinformatic methods to identify the bacterial isolates. Throughout the course, students learn about the process of generating biofuels and bioproducts through the deconstruction of cellulosic biomass to form monosaccharides from the biopolymers in plant matter. The program relies heavily on active learning and enables students to connect microbiology with issues of sustainability. In addition, it provides exposure to basic microbiology, molecular biology, and biotechnology laboratory techniques and concepts. The described activity was initially developed for the Introductory College Level Experience in Microbiology (iCLEM) program, a research-based immersive laboratory course at the US Department of Energy Joint BioEnergy Institute. Originally designed as an accelerated program for high-potential, low-income, high school students (11th–12th grade), this curriculum could also be implemented for undergraduate coursework in a research-intensive laboratory course at a two- or four-year college or university.Jesus F. BarajasMaren WehrsMilton ToLauchlin CruickshanksRochelle UrbanAdrienne McKeeJohn GladdenEe-Been GohMargaret E. BrownDiane PierottiJames M. CarothersAindrila MukhopadhyayJay D. KeaslingJeffrey L. FortmanSteven W. SingerConstance B. BaileyAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 20, Iss 2 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Jesus F. Barajas Maren Wehrs Milton To Lauchlin Cruickshanks Rochelle Urban Adrienne McKee John Gladden Ee-Been Goh Margaret E. Brown Diane Pierotti James M. Carothers Aindrila Mukhopadhyay Jay D. Keasling Jeffrey L. Fortman Steven W. Singer Constance B. Bailey Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course
description	The conversion of biomass to biofuels presents a solution to one of the largest global challenges of our era, climate change. A critical part of this pipeline is the process of breaking down cellulosic sugars from plant matter to be used by microbes containing biosynthetic pathways that produce biofuels or bioproducts. In this inquiry-based course, students complete a research project that isolates cellulase-producing bacteria from samples collected from the environment. After obtaining isolates, the students characterize the production of cellulases. Students then amplify and sequence the 16S rRNA genes of confirmed cellulase producers and use bioinformatic methods to identify the bacterial isolates. Throughout the course, students learn about the process of generating biofuels and bioproducts through the deconstruction of cellulosic biomass to form monosaccharides from the biopolymers in plant matter. The program relies heavily on active learning and enables students to connect microbiology with issues of sustainability. In addition, it provides exposure to basic microbiology, molecular biology, and biotechnology laboratory techniques and concepts. The described activity was initially developed for the Introductory College Level Experience in Microbiology (iCLEM) program, a research-based immersive laboratory course at the US Department of Energy Joint BioEnergy Institute. Originally designed as an accelerated program for high-potential, low-income, high school students (11th–12th grade), this curriculum could also be implemented for undergraduate coursework in a research-intensive laboratory course at a two- or four-year college or university.
format	article
author	Jesus F. Barajas Maren Wehrs Milton To Lauchlin Cruickshanks Rochelle Urban Adrienne McKee John Gladden Ee-Been Goh Margaret E. Brown Diane Pierotti James M. Carothers Aindrila Mukhopadhyay Jay D. Keasling Jeffrey L. Fortman Steven W. Singer Constance B. Bailey
author_facet	Jesus F. Barajas Maren Wehrs Milton To Lauchlin Cruickshanks Rochelle Urban Adrienne McKee John Gladden Ee-Been Goh Margaret E. Brown Diane Pierotti James M. Carothers Aindrila Mukhopadhyay Jay D. Keasling Jeffrey L. Fortman Steven W. Singer Constance B. Bailey
author_sort	Jesus F. Barajas
title	Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course
title_short	Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course
title_full	Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course
title_fullStr	Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course
title_full_unstemmed	Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course
title_sort	isolation and characterization of bacterial cellulase producers for biomass deconstruction: a microbiology laboratory course
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/398f5e54588c411ca5f1dbbd33d8bc7a
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Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course

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