Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells

Abstract Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BN...

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Autores principales:	Julia Rudolph, Matthias Völkl, Valérie Jérôme, Thomas Scheibel, Ruth Freitag
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39908e4d9fc74b39a785d631c4c999ee
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spelling	oai:doaj.org-article:39908e4d9fc74b39a785d631c4c999ee2021-12-02T16:36:36ZNoxic effects of polystyrene microparticles on murine macrophages and epithelial cells10.1038/s41598-021-95073-92045-2322https://doaj.org/article/39908e4d9fc74b39a785d631c4c999ee2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95073-9https://doaj.org/toc/2045-2322Abstract Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions.Julia RudolphMatthias VölklValérie JérômeThomas ScheibelRuth FreitagNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julia Rudolph Matthias Völkl Valérie Jérôme Thomas Scheibel Ruth Freitag Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
description	Abstract Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions.
format	article
author	Julia Rudolph Matthias Völkl Valérie Jérôme Thomas Scheibel Ruth Freitag
author_facet	Julia Rudolph Matthias Völkl Valérie Jérôme Thomas Scheibel Ruth Freitag
author_sort	Julia Rudolph
title	Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
title_short	Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
title_full	Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
title_fullStr	Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
title_full_unstemmed	Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
title_sort	noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/39908e4d9fc74b39a785d631c4c999ee
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Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells

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