The Drosophila Baramicin polypeptide gene protects against fungal infection.

The fruit fly Drosophila melanogaster combats microbial infection by producing a battery of effector peptides that are secreted into the haemolymph. Technical difficulties prevented the investigation of these short effector genes until the recent advent of the CRISPR/CAS era. As a consequence, many...

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Autores principales:	Mark Austin Hanson, Lianne B Cohen, Alice Marra, Igor Iatsenko, Steven A Wasserman, Bruno Lemaitre
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/39999117db4448b790680c9f75fec9c9
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spelling	oai:doaj.org-article:39999117db4448b790680c9f75fec9c92021-12-02T20:00:16ZThe Drosophila Baramicin polypeptide gene protects against fungal infection.1553-73661553-737410.1371/journal.ppat.1009846https://doaj.org/article/39999117db4448b790680c9f75fec9c92021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009846https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The fruit fly Drosophila melanogaster combats microbial infection by producing a battery of effector peptides that are secreted into the haemolymph. Technical difficulties prevented the investigation of these short effector genes until the recent advent of the CRISPR/CAS era. As a consequence, many putative immune effectors remain to be formally described, and exactly how each of these effectors contribute to survival is not well characterized. Here we describe a novel Drosophila antifungal peptide gene that we name Baramicin A. We show that BaraA encodes a precursor protein cleaved into multiple peptides via furin cleavage sites. BaraA is strongly immune-induced in the fat body downstream of the Toll pathway, but also exhibits expression in other tissues. Importantly, we show that flies lacking BaraA are viable but susceptible to the entomopathogenic fungus Beauveria bassiana. Consistent with BaraA being directly antimicrobial, overexpression of BaraA promotes resistance to fungi and the IM10-like peptides produced by BaraA synergistically inhibit growth of fungi in vitro when combined with a membrane-disrupting antifungal. Surprisingly, BaraA mutant males but not females display an erect wing phenotype upon infection. Here, we characterize a new antifungal immune effector downstream of Toll signalling, and show it is a key contributor to the Drosophila antimicrobial response.Mark Austin HansonLianne B CohenAlice MarraIgor IatsenkoSteven A WassermanBruno LemaitrePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 8, p e1009846 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Mark Austin Hanson Lianne B Cohen Alice Marra Igor Iatsenko Steven A Wasserman Bruno Lemaitre The Drosophila Baramicin polypeptide gene protects against fungal infection.
description	The fruit fly Drosophila melanogaster combats microbial infection by producing a battery of effector peptides that are secreted into the haemolymph. Technical difficulties prevented the investigation of these short effector genes until the recent advent of the CRISPR/CAS era. As a consequence, many putative immune effectors remain to be formally described, and exactly how each of these effectors contribute to survival is not well characterized. Here we describe a novel Drosophila antifungal peptide gene that we name Baramicin A. We show that BaraA encodes a precursor protein cleaved into multiple peptides via furin cleavage sites. BaraA is strongly immune-induced in the fat body downstream of the Toll pathway, but also exhibits expression in other tissues. Importantly, we show that flies lacking BaraA are viable but susceptible to the entomopathogenic fungus Beauveria bassiana. Consistent with BaraA being directly antimicrobial, overexpression of BaraA promotes resistance to fungi and the IM10-like peptides produced by BaraA synergistically inhibit growth of fungi in vitro when combined with a membrane-disrupting antifungal. Surprisingly, BaraA mutant males but not females display an erect wing phenotype upon infection. Here, we characterize a new antifungal immune effector downstream of Toll signalling, and show it is a key contributor to the Drosophila antimicrobial response.
format	article
author	Mark Austin Hanson Lianne B Cohen Alice Marra Igor Iatsenko Steven A Wasserman Bruno Lemaitre
author_facet	Mark Austin Hanson Lianne B Cohen Alice Marra Igor Iatsenko Steven A Wasserman Bruno Lemaitre
author_sort	Mark Austin Hanson
title	The Drosophila Baramicin polypeptide gene protects against fungal infection.
title_short	The Drosophila Baramicin polypeptide gene protects against fungal infection.
title_full	The Drosophila Baramicin polypeptide gene protects against fungal infection.
title_fullStr	The Drosophila Baramicin polypeptide gene protects against fungal infection.
title_full_unstemmed	The Drosophila Baramicin polypeptide gene protects against fungal infection.
title_sort	drosophila baramicin polypeptide gene protects against fungal infection.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/39999117db4448b790680c9f75fec9c9
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The Drosophila Baramicin polypeptide gene protects against fungal infection.

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