Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance

Abstract Metal tolerance is often a result of metal storage or distribution. Thus, with the goal of advancing the molecular understanding of such metal homeostatic mechanisms, natural variation of metal tolerance in Arabidopsis thaliana was investigated. Substantial variation exists in tolerance of...

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Autores principales:	Sina Fischer, Thomas Spielau, Stephan Clemens
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/399c9ff3289c409a917f2556e445fee4
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spelling	oai:doaj.org-article:399c9ff3289c409a917f2556e445fee42021-12-02T15:04:56ZNatural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance10.1038/s41598-017-03540-z2045-2322https://doaj.org/article/399c9ff3289c409a917f2556e445fee42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03540-zhttps://doaj.org/toc/2045-2322Abstract Metal tolerance is often a result of metal storage or distribution. Thus, with the goal of advancing the molecular understanding of such metal homeostatic mechanisms, natural variation of metal tolerance in Arabidopsis thaliana was investigated. Substantial variation exists in tolerance of excess copper (Cu), zinc (Zn) and cadmium (Cd). Two accessions, Col-0 and Bur-0, and a recombinant inbred line (RIL) population derived from these parents were chosen for further analysis of Cd and Zn tolerance variation, which is evident at different plant ages in various experimental systems and appears to be genetically linked. Three QTLs, explaining in total nearly 50% of the variation in Cd tolerance, were mapped. The one obvious candidate gene in the mapped intervals, HMA3, is unlikely to contribute to the variation. In order to identify additional candidate genes the Cd responses of Col-0 and Bur-0 were compared at the transcriptome level. The sustained common Cd response of the two accessions was dominated by processes implicated in plant pathogen defense. Accession-specific differences suggested a more efficient activation of acclimative responses as underlying the higher Cd tolerance of Bur-0. The second hypothesis derived from the physiological characterization of the accessions is a reduced Cd accumulation in Bur-0.Sina FischerThomas SpielauStephan ClemensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sina Fischer Thomas Spielau Stephan Clemens Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance
description	Abstract Metal tolerance is often a result of metal storage or distribution. Thus, with the goal of advancing the molecular understanding of such metal homeostatic mechanisms, natural variation of metal tolerance in Arabidopsis thaliana was investigated. Substantial variation exists in tolerance of excess copper (Cu), zinc (Zn) and cadmium (Cd). Two accessions, Col-0 and Bur-0, and a recombinant inbred line (RIL) population derived from these parents were chosen for further analysis of Cd and Zn tolerance variation, which is evident at different plant ages in various experimental systems and appears to be genetically linked. Three QTLs, explaining in total nearly 50% of the variation in Cd tolerance, were mapped. The one obvious candidate gene in the mapped intervals, HMA3, is unlikely to contribute to the variation. In order to identify additional candidate genes the Cd responses of Col-0 and Bur-0 were compared at the transcriptome level. The sustained common Cd response of the two accessions was dominated by processes implicated in plant pathogen defense. Accession-specific differences suggested a more efficient activation of acclimative responses as underlying the higher Cd tolerance of Bur-0. The second hypothesis derived from the physiological characterization of the accessions is a reduced Cd accumulation in Bur-0.
format	article
author	Sina Fischer Thomas Spielau Stephan Clemens
author_facet	Sina Fischer Thomas Spielau Stephan Clemens
author_sort	Sina Fischer
title	Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance
title_short	Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance
title_full	Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance
title_fullStr	Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance
title_full_unstemmed	Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance
title_sort	natural variation in arabidopsis thaliana cd responses and the detection of quantitative trait loci affecting cd tolerance
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/399c9ff3289c409a917f2556e445fee4
work_keys_str_mv	AT sinafischer naturalvariationinarabidopsisthalianacdresponsesandthedetectionofquantitativetraitlociaffectingcdtolerance AT thomasspielau naturalvariationinarabidopsisthalianacdresponsesandthedetectionofquantitativetraitlociaffectingcdtolerance AT stephanclemens naturalvariationinarabidopsisthalianacdresponsesandthedetectionofquantitativetraitlociaffectingcdtolerance
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Natural variation in Arabidopsis thaliana Cd responses and the detection of quantitative trait loci affecting Cd tolerance

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