Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats

Abstract Maternal consumption of polyphenol-rich foods has been associated with fetal ductus arteriosus constriction (DAC), but safety of chocolate exposure in fetal life has not been studied. This experimental study tested the hypothesis that maternal cocoa consumption in late pregnancy causes feta...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Paulo Zielinsky, Felipe Villa Martignoni, Melissa Markoski, Kelly Pozzer Zucatti, Gabriela dos Santos Marinho, Gabriela Pozzobon, Pedro Rafael Magno, Victória de Bittencourt Antunes, Natassia Miranda Sulis, Alexandra Cardoso, Daniel Mattos, Alexandre Antônio Naujorks, Anize Delfino von Frankenberg, Izabele Vian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39a43671a24e4d068899cc8fb501cc57
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:39a43671a24e4d068899cc8fb501cc57
record_format	dspace
spelling	oai:doaj.org-article:39a43671a24e4d068899cc8fb501cc572021-12-02T15:36:13ZMaternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats10.1038/s41598-021-89309-x2045-2322https://doaj.org/article/39a43671a24e4d068899cc8fb501cc572021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89309-xhttps://doaj.org/toc/2045-2322Abstract Maternal consumption of polyphenol-rich foods has been associated with fetal ductus arteriosus constriction (DAC), but safety of chocolate exposure in fetal life has not been studied. This experimental study tested the hypothesis that maternal cocoa consumption in late pregnancy causes fetal DAC, with possible associated antioxidant effects. Pregnant Wistar rats, at the 21st gestational day, received by orogastric tube cocoa (720 mg/Kg) for 12 h, indomethacin (10 mg/Kg), for 8 h, or only water, before cesaren section. Immediately after withdrawal, every thorax was obtained and tissues were fixed and stained for histological analysis. The ratio of the narrowest part of the pulmonary artery to the fetal ductus inner diameter and increased ductal inner wall thickness characterized ductal constriction. Substances reactive to thiobarbituric acid were quantified. Statistical analysis used ANOVA and Tukey test. Cocoa (n = 33) and indomethacin (n = 7) reduced fetal internal ductus diameter when compared to control (water, n = 25) (p < 0.001) and cocoa alone increased ductus wall thickness (p < 0.001), but no change was noted in enzymes activity. This pharmacological study shows supporting evidences that there is a cause and effect relationship between maternal consumption of cocoa and fetal ductus arteriosus constriction. Habitual widespread use of chocolate during gestation could account for undetected ductus constriction and its potentially severe consequences, such as perinatal pulmonary hypertension, cardiac failure and even death. For this reason, dietary guidance in late pregnancy to avoid high chocolate intake, to prevent fetal ductal constriction, may represent the main translational aspect of this study.Paulo ZielinskyFelipe Villa MartignoniMelissa MarkoskiKelly Pozzer ZucattiGabriela dos Santos MarinhoGabriela PozzobonPedro Rafael MagnoVictória de Bittencourt AntunesNatassia Miranda SulisAlexandra CardosoDaniel MattosAlexandre Antônio NaujorksAnize Delfino von FrankenbergIzabele VianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paulo Zielinsky Felipe Villa Martignoni Melissa Markoski Kelly Pozzer Zucatti Gabriela dos Santos Marinho Gabriela Pozzobon Pedro Rafael Magno Victória de Bittencourt Antunes Natassia Miranda Sulis Alexandra Cardoso Daniel Mattos Alexandre Antônio Naujorks Anize Delfino von Frankenberg Izabele Vian Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
description	Abstract Maternal consumption of polyphenol-rich foods has been associated with fetal ductus arteriosus constriction (DAC), but safety of chocolate exposure in fetal life has not been studied. This experimental study tested the hypothesis that maternal cocoa consumption in late pregnancy causes fetal DAC, with possible associated antioxidant effects. Pregnant Wistar rats, at the 21st gestational day, received by orogastric tube cocoa (720 mg/Kg) for 12 h, indomethacin (10 mg/Kg), for 8 h, or only water, before cesaren section. Immediately after withdrawal, every thorax was obtained and tissues were fixed and stained for histological analysis. The ratio of the narrowest part of the pulmonary artery to the fetal ductus inner diameter and increased ductal inner wall thickness characterized ductal constriction. Substances reactive to thiobarbituric acid were quantified. Statistical analysis used ANOVA and Tukey test. Cocoa (n = 33) and indomethacin (n = 7) reduced fetal internal ductus diameter when compared to control (water, n = 25) (p < 0.001) and cocoa alone increased ductus wall thickness (p < 0.001), but no change was noted in enzymes activity. This pharmacological study shows supporting evidences that there is a cause and effect relationship between maternal consumption of cocoa and fetal ductus arteriosus constriction. Habitual widespread use of chocolate during gestation could account for undetected ductus constriction and its potentially severe consequences, such as perinatal pulmonary hypertension, cardiac failure and even death. For this reason, dietary guidance in late pregnancy to avoid high chocolate intake, to prevent fetal ductal constriction, may represent the main translational aspect of this study.
format	article
author	Paulo Zielinsky Felipe Villa Martignoni Melissa Markoski Kelly Pozzer Zucatti Gabriela dos Santos Marinho Gabriela Pozzobon Pedro Rafael Magno Victória de Bittencourt Antunes Natassia Miranda Sulis Alexandra Cardoso Daniel Mattos Alexandre Antônio Naujorks Anize Delfino von Frankenberg Izabele Vian
author_facet	Paulo Zielinsky Felipe Villa Martignoni Melissa Markoski Kelly Pozzer Zucatti Gabriela dos Santos Marinho Gabriela Pozzobon Pedro Rafael Magno Victória de Bittencourt Antunes Natassia Miranda Sulis Alexandra Cardoso Daniel Mattos Alexandre Antônio Naujorks Anize Delfino von Frankenberg Izabele Vian
author_sort	Paulo Zielinsky
title	Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
title_short	Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
title_full	Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
title_fullStr	Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
title_full_unstemmed	Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
title_sort	maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/39a43671a24e4d068899cc8fb501cc57
work_keys_str_mv	AT paulozielinsky maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT felipevillamartignoni maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT melissamarkoski maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT kellypozzerzucatti maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT gabrieladossantosmarinho maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT gabrielapozzobon maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT pedrorafaelmagno maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT victoriadebittencourtantunes maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT natassiamirandasulis maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT alexandracardoso maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT danielmattos maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT alexandreantonionaujorks maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT anizedelfinovonfrankenberg maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats AT izabelevian maternalingestionofcocoacausesconstrictionoffetalductusarteriosusinrats
_version_	1718386346371842048

Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats

Ejemplares similares