Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities

The pumpkin pulp contains a greater composition of edible polysaccharides and has reported with excellent biological applications. This research pertains to optimize the extraction of polysaccharides from the fleshy portion of the pumpkin using aqueous assisted extraction (AAE). The result showed th...

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Autores principales:	Saraswathi Umavathi, Madhayan Keerthika, Kasi Gopinath, Chandramohan Kavitha, Md. Romij Uddin, Shanmugam Alagumanian, Chinnasamy Balalakshmi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Pumpkin Polysaccharides α-amylase inhibitory effect Anti-inflammatory effect Antioxidant activity Antimicrobial activity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/39aa0aec7a2a41129494c9edaf786bb0
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spelling	oai:doaj.org-article:39aa0aec7a2a41129494c9edaf786bb02021-11-20T04:56:20ZOptimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities1319-562X10.1016/j.sjbs.2021.07.034https://doaj.org/article/39aa0aec7a2a41129494c9edaf786bb02021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006070https://doaj.org/toc/1319-562XThe pumpkin pulp contains a greater composition of edible polysaccharides and has reported with excellent biological applications. This research pertains to optimize the extraction of polysaccharides from the fleshy portion of the pumpkin using aqueous assisted extraction (AAE). The result showed that the optimal extraction condition of pumpkin polysaccharide was as follows: extraction temperature at 55 °C, pH 4.5, and enzyme concentration of 4000 µ/g for 80 min. Under the optimal extraction condition, the yield of pumpkin polysaccharide via AAE (15.4) was significantly higher. The biological activities of extracted polysaccharide including α-amylase inhibition (57.41% at 1000 µg/mL) and anti-inflammatory (50.41% at 25 µg/mL) activity increased significantly. Additionally, the antioxidant activities of extracted pumpkin polysaccharides including IC50 values of DPPH and ABTS were 59.87% and 58.74%, respectively. The pumpkin polysaccharide has maximum inhibitory effects against bacterial strains especially for Escherichia coli than that of fungal strains. It is suggested that the aqueous assisted extraction of is a cost-effective promising method to decrease the processing time as well as enhancing extracted polysaccharide yield – times.Saraswathi UmavathiMadhayan KeerthikaKasi GopinathChandramohan KavithaMd. Romij UddinShanmugam AlagumanianChinnasamy BalalakshmiElsevierarticlePumpkin Polysaccharidesα-amylase inhibitory effectAnti-inflammatory effectAntioxidant activityAntimicrobial activityBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6692-6700 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pumpkin Polysaccharides α-amylase inhibitory effect Anti-inflammatory effect Antioxidant activity Antimicrobial activity Biology (General) QH301-705.5
spellingShingle	Pumpkin Polysaccharides α-amylase inhibitory effect Anti-inflammatory effect Antioxidant activity Antimicrobial activity Biology (General) QH301-705.5 Saraswathi Umavathi Madhayan Keerthika Kasi Gopinath Chandramohan Kavitha Md. Romij Uddin Shanmugam Alagumanian Chinnasamy Balalakshmi Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities
description	The pumpkin pulp contains a greater composition of edible polysaccharides and has reported with excellent biological applications. This research pertains to optimize the extraction of polysaccharides from the fleshy portion of the pumpkin using aqueous assisted extraction (AAE). The result showed that the optimal extraction condition of pumpkin polysaccharide was as follows: extraction temperature at 55 °C, pH 4.5, and enzyme concentration of 4000 µ/g for 80 min. Under the optimal extraction condition, the yield of pumpkin polysaccharide via AAE (15.4) was significantly higher. The biological activities of extracted polysaccharide including α-amylase inhibition (57.41% at 1000 µg/mL) and anti-inflammatory (50.41% at 25 µg/mL) activity increased significantly. Additionally, the antioxidant activities of extracted pumpkin polysaccharides including IC50 values of DPPH and ABTS were 59.87% and 58.74%, respectively. The pumpkin polysaccharide has maximum inhibitory effects against bacterial strains especially for Escherichia coli than that of fungal strains. It is suggested that the aqueous assisted extraction of is a cost-effective promising method to decrease the processing time as well as enhancing extracted polysaccharide yield – times.
format	article
author	Saraswathi Umavathi Madhayan Keerthika Kasi Gopinath Chandramohan Kavitha Md. Romij Uddin Shanmugam Alagumanian Chinnasamy Balalakshmi
author_facet	Saraswathi Umavathi Madhayan Keerthika Kasi Gopinath Chandramohan Kavitha Md. Romij Uddin Shanmugam Alagumanian Chinnasamy Balalakshmi
author_sort	Saraswathi Umavathi
title	Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities
title_short	Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities
title_full	Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities
title_fullStr	Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities
title_full_unstemmed	Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities
title_sort	optimization of aqueous-assisted extraction of polysaccharides from pumpkin (cucurbita moschata duch) and their biological activities
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/39aa0aec7a2a41129494c9edaf786bb0
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Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities

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