Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity

Bin Liang,1,* Na Li,2,* Shuofei Zhang,3 Aihua Qi,4 Jianhua Feng,1 Weiwei Jing,5 Changcan Shi,2 Zhaipu Ma,6 Shenmeng Gao7 1Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang 325000, China; 2Wenzhou Institute of Biomat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liang B, Li N, Zhang S, Qi A, Feng J, Jing W, Shi C, Ma Z, Gao S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Idarubicin mPEG-PLGA Acute myeloid leukemia Nanoparticles Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/39af56e5894b4661b2178eb03440cfec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:39af56e5894b4661b2178eb03440cfec
record_format	dspace
spelling	oai:doaj.org-article:39af56e5894b4661b2178eb03440cfec2021-12-02T07:29:44ZIdarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity1178-2013https://doaj.org/article/39af56e5894b4661b2178eb03440cfec2019-01-01T00:00:00Zhttps://www.dovepress.com/idarubicin-loaded-methoxy-polyethylene-glycol-b-polyl-lactide-co-glyco-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Bin Liang,1,* Na Li,2,* Shuofei Zhang,3 Aihua Qi,4 Jianhua Feng,1 Weiwei Jing,5 Changcan Shi,2 Zhaipu Ma,6 Shenmeng Gao7 1Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang 325000, China; 2Wenzhou Institute of Biomaterials and Engineering, CNITECH, CAS, Wenzhou, Zhejiang 325000, China; 3Department of Orthodontics, Stomatological Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; 4Department of Internal Medicine, Zaoqiang People’s Hospital, Zaoqiang, Hebei 053100, China; 5Department of Obstetrics and Gynecology, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou, Zhejiang 325000, China; 6Department of Bioinformatics, College of Life Science, Hebei University, Baoding, Hebei 071002, China; 7Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang 325000, China *These authors contributed equally to this work Purpose: Nanoparticle (NP)-based drug delivery approaches have tremendous potential for enhancing treatment efficacy and decreasing doses of chemotherapeutics. Idarubicin (IDA) is one of the most common chemotherapeutic drugs used in the treatment of acute myeloid leukemia (AML). However, severe side effects and drug resistance markedly limit the application of IDA.Methods: In this study, we encapsulated IDA in polymeric NPs and validated their antileukemia activity in vitro and in vivo.Results: NPs with an average diameter of 84 nm was assembled from a methoxy poly(ethylene glycol)-b-poly(l-lactide-co-glycolide) (mPEG-PLGA). After loading of IDA, IDA-loaded mPEG-PLGA NPs (IDA/mPEG-PLGA NPs) were formed. The in vitro release data showed that the IDA/mPEG-PLGA NPs have excellent sustained release property. IDA/mPEG-PLGA NPs had exhibited the lower IC50 than pure IDA. Moreover, IDA/mPEG-PLGA NPs in the same concentration substantially induced apoptosis than did pure IDA. Most importantly, IDA/MPEG-PLGA NPs significantly decreased the infiltration of leukemia blasts and improved the overall survival of MLL-AF9-induced murine leukemia compared with free IDA. However, the blank NPs were nontoxic to normal cultured cells in vitro, suggesting that NPs were the safe carrier.Conclusion: Our data suggest that IDA/mPEG-PLGA NPs might be a suitable carrier to encapsulate IDA. Low dose of IDA/mPEG-PLGA NPs can be used as a conventional dosage for antileukemia therapy to reduce side effect and improve survival. Keywords: idarubicin, mPEG-PLGA, acute myeloid leukemia, nanoparticlesLiang BLi NZhang SQi AFeng JJing WShi CMa ZGao SDove Medical PressarticleIdarubicinmPEG-PLGAAcute myeloid leukemiaNanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 543-556 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Idarubicin mPEG-PLGA Acute myeloid leukemia Nanoparticles Medicine (General) R5-920
spellingShingle	Idarubicin mPEG-PLGA Acute myeloid leukemia Nanoparticles Medicine (General) R5-920 Liang B Li N Zhang S Qi A Feng J Jing W Shi C Ma Z Gao S Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity
description	Bin Liang,1,* Na Li,2,* Shuofei Zhang,3 Aihua Qi,4 Jianhua Feng,1 Weiwei Jing,5 Changcan Shi,2 Zhaipu Ma,6 Shenmeng Gao7 1Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang 325000, China; 2Wenzhou Institute of Biomaterials and Engineering, CNITECH, CAS, Wenzhou, Zhejiang 325000, China; 3Department of Orthodontics, Stomatological Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China; 4Department of Internal Medicine, Zaoqiang People’s Hospital, Zaoqiang, Hebei 053100, China; 5Department of Obstetrics and Gynecology, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou, Zhejiang 325000, China; 6Department of Bioinformatics, College of Life Science, Hebei University, Baoding, Hebei 071002, China; 7Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang 325000, China *These authors contributed equally to this work Purpose: Nanoparticle (NP)-based drug delivery approaches have tremendous potential for enhancing treatment efficacy and decreasing doses of chemotherapeutics. Idarubicin (IDA) is one of the most common chemotherapeutic drugs used in the treatment of acute myeloid leukemia (AML). However, severe side effects and drug resistance markedly limit the application of IDA.Methods: In this study, we encapsulated IDA in polymeric NPs and validated their antileukemia activity in vitro and in vivo.Results: NPs with an average diameter of 84 nm was assembled from a methoxy poly(ethylene glycol)-b-poly(l-lactide-co-glycolide) (mPEG-PLGA). After loading of IDA, IDA-loaded mPEG-PLGA NPs (IDA/mPEG-PLGA NPs) were formed. The in vitro release data showed that the IDA/mPEG-PLGA NPs have excellent sustained release property. IDA/mPEG-PLGA NPs had exhibited the lower IC50 than pure IDA. Moreover, IDA/mPEG-PLGA NPs in the same concentration substantially induced apoptosis than did pure IDA. Most importantly, IDA/MPEG-PLGA NPs significantly decreased the infiltration of leukemia blasts and improved the overall survival of MLL-AF9-induced murine leukemia compared with free IDA. However, the blank NPs were nontoxic to normal cultured cells in vitro, suggesting that NPs were the safe carrier.Conclusion: Our data suggest that IDA/mPEG-PLGA NPs might be a suitable carrier to encapsulate IDA. Low dose of IDA/mPEG-PLGA NPs can be used as a conventional dosage for antileukemia therapy to reduce side effect and improve survival. Keywords: idarubicin, mPEG-PLGA, acute myeloid leukemia, nanoparticles
format	article
author	Liang B Li N Zhang S Qi A Feng J Jing W Shi C Ma Z Gao S
author_facet	Liang B Li N Zhang S Qi A Feng J Jing W Shi C Ma Z Gao S
author_sort	Liang B
title	Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity
title_short	Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity
title_full	Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity
title_fullStr	Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity
title_full_unstemmed	Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity
title_sort	idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(l-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/39af56e5894b4661b2178eb03440cfec
work_keys_str_mv	AT liangb idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT lin idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT zhangs idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT qia idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT fengj idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT jingw idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT shic idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT maz idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity AT gaos idarubicinloadedmethoxypolyethyleneglycolbpolyllactidecoglycolidenanoparticlesforenhancingcellularuptakeandpromotingantileukemiaactivity
_version_	1718399394076688384

Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(L-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity

Ejemplares similares