Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime

ABSTRACT Mycoplasma mobile, a fish-pathogenic bacterium, features a protrusion that enables it to glide smoothly on solid surfaces at a velocity of up to 4.5 µm s−1 in the direction of the protrusion. M. mobile glides by a repeated catch-pull-release of sialylated oligosaccharides fixed on a solid s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Akihiro Tanaka, Daisuke Nakane, Masaki Mizutani, Takayuki Nishizaka, Makoto Miyata
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/39bc5aeb225240589e222309ea0cfca7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:39bc5aeb225240589e222309ea0cfca7
record_format	dspace
spelling	oai:doaj.org-article:39bc5aeb225240589e222309ea0cfca72021-11-15T15:50:16ZDirected Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime10.1128/mBio.00455-162150-7511https://doaj.org/article/39bc5aeb225240589e222309ea0cfca72016-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00455-16https://doaj.org/toc/2150-7511ABSTRACT Mycoplasma mobile, a fish-pathogenic bacterium, features a protrusion that enables it to glide smoothly on solid surfaces at a velocity of up to 4.5 µm s−1 in the direction of the protrusion. M. mobile glides by a repeated catch-pull-release of sialylated oligosaccharides fixed on a solid surface by hundreds of 50-nm flexible “legs” sticking out from the protrusion. This gliding mechanism may be explained by a possible directed binding of each leg with sialylated oligosaccharides, by which the leg can be detached more easily forward than backward. In the present study, we used a polystyrene bead held by optical tweezers to detach a starved cell at rest from a glass surface coated with sialylated oligosaccharides and concluded that the detachment force forward is 1.6- to 1.8-fold less than that backward, which may be linked to a catch bond-like behavior of the cell. These results suggest that this directed binding has a critical role in the gliding mechanism. IMPORTANCE Mycoplasma species are the smallest bacteria and are parasitic and occasionally commensal, as represented by Mycoplasma pneumoniae, which causes so-called “walking pneumonia” in humans. Dozens of species glide on host tissues, always in the direction of the characteristic cellular protrusion, by novel mechanisms. The fastest species, Mycoplasma mobile, catches, pulls, and releases sialylated oligosaccharides (SOs), which are common targets among influenza viruses, by means of a specific receptor based on the energy of ATP hydrolysis. Here, force measurements made with optical tweezers revealed that the force required to detach a cell from SOs is smaller forward than backward along the gliding direction. The directed binding should be a clue to elucidate this novel motility mechanism.Akihiro TanakaDaisuke NakaneMasaki MizutaniTakayuki NishizakaMakoto MiyataAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 3 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Akihiro Tanaka Daisuke Nakane Masaki Mizutani Takayuki Nishizaka Makoto Miyata Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime
description	ABSTRACT Mycoplasma mobile, a fish-pathogenic bacterium, features a protrusion that enables it to glide smoothly on solid surfaces at a velocity of up to 4.5 µm s−1 in the direction of the protrusion. M. mobile glides by a repeated catch-pull-release of sialylated oligosaccharides fixed on a solid surface by hundreds of 50-nm flexible “legs” sticking out from the protrusion. This gliding mechanism may be explained by a possible directed binding of each leg with sialylated oligosaccharides, by which the leg can be detached more easily forward than backward. In the present study, we used a polystyrene bead held by optical tweezers to detach a starved cell at rest from a glass surface coated with sialylated oligosaccharides and concluded that the detachment force forward is 1.6- to 1.8-fold less than that backward, which may be linked to a catch bond-like behavior of the cell. These results suggest that this directed binding has a critical role in the gliding mechanism. IMPORTANCE Mycoplasma species are the smallest bacteria and are parasitic and occasionally commensal, as represented by Mycoplasma pneumoniae, which causes so-called “walking pneumonia” in humans. Dozens of species glide on host tissues, always in the direction of the characteristic cellular protrusion, by novel mechanisms. The fastest species, Mycoplasma mobile, catches, pulls, and releases sialylated oligosaccharides (SOs), which are common targets among influenza viruses, by means of a specific receptor based on the energy of ATP hydrolysis. Here, force measurements made with optical tweezers revealed that the force required to detach a cell from SOs is smaller forward than backward along the gliding direction. The directed binding should be a clue to elucidate this novel motility mechanism.
format	article
author	Akihiro Tanaka Daisuke Nakane Masaki Mizutani Takayuki Nishizaka Makoto Miyata
author_facet	Akihiro Tanaka Daisuke Nakane Masaki Mizutani Takayuki Nishizaka Makoto Miyata
author_sort	Akihiro Tanaka
title	Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime
title_short	Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime
title_full	Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime
title_fullStr	Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime
title_full_unstemmed	Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime
title_sort	directed binding of gliding bacterium, <named-content content-type="genus-species">mycoplasma mobile</named-content>, shown by detachment force and bond lifetime
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/39bc5aeb225240589e222309ea0cfca7
work_keys_str_mv	AT akihirotanaka directedbindingofglidingbacteriumnamedcontentcontenttypegenusspeciesmycoplasmamobilenamedcontentshownbydetachmentforceandbondlifetime AT daisukenakane directedbindingofglidingbacteriumnamedcontentcontenttypegenusspeciesmycoplasmamobilenamedcontentshownbydetachmentforceandbondlifetime AT masakimizutani directedbindingofglidingbacteriumnamedcontentcontenttypegenusspeciesmycoplasmamobilenamedcontentshownbydetachmentforceandbondlifetime AT takayukinishizaka directedbindingofglidingbacteriumnamedcontentcontenttypegenusspeciesmycoplasmamobilenamedcontentshownbydetachmentforceandbondlifetime AT makotomiyata directedbindingofglidingbacteriumnamedcontentcontenttypegenusspeciesmycoplasmamobilenamedcontentshownbydetachmentforceandbondlifetime
_version_	1718427452141731840

Directed Binding of Gliding Bacterium, <named-content content-type="genus-species">Mycoplasma mobile</named-content>, Shown by Detachment Force and Bond Lifetime

Ejemplares similares