Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities

Shousi Lu,1,2 Shanshan Guo,1 Pingxiang Xu,1 Xiaorong Li,1 Yuming Zhao,1 Wei Gu,3 Ming Xue1 1Department of Pharmacology, Beijing Laboratory for Biomedical Detection Technology and Instrument, School of Basic Medical Sciences, Capital Medical University, 2China Rehabilitation Research Center, 3Depart...

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Autores principales:	Lu SS, Guo SS, Xu PX, Li XR, Zhao YM, Gu W, Xue M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	hydrothermal synthesis nitrogen-doped carbon dots bioimaging blood brain barrier Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/39c01391d494426aad9c1a358b691f1c
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spelling	oai:doaj.org-article:39c01391d494426aad9c1a358b691f1c2021-12-02T03:11:38ZHydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities1178-2013https://doaj.org/article/39c01391d494426aad9c1a358b691f1c2016-11-01T00:00:00Zhttps://www.dovepress.com/hydrothermal-synthesis-of-nitrogen-doped-carbon-dots-with-real-time-li-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shousi Lu,1,2 Shanshan Guo,1 Pingxiang Xu,1 Xiaorong Li,1 Yuming Zhao,1 Wei Gu,3 Ming Xue1 1Department of Pharmacology, Beijing Laboratory for Biomedical Detection Technology and Instrument, School of Basic Medical Sciences, Capital Medical University, 2China Rehabilitation Research Center, 3Department of Chemistry and Biology, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, China Abstract: Nitrogen-doped carbon dots (N-CDs) were synthesized using a one-pot hydrothermal treatment with citric acid in the presence of polyethylenimine. Transmission electron microscopy analysis revealed that the N-CDs were monodispersed and quasi-spherical with an average size of ~2.6 nm. Under ultraviolet irradiation the N-CDs emitted a strong blue luminescence with a quantum yield as high as 51%. Moreover, the N-CDs exhibited a negligible cytotoxicity and could be applied as efficient nanoprobes for real-time imaging of live cells. In addition, the ability of the N-CDs to cross the blood–brain barrier (BBB) in a concentration-dependent manner was demonstrated using an in vitro BBB model. Therefore, these PEI-passivated N-CDs with real-time live-cell imaging and BBB-penetration capabilities hold promise for traceable drug delivery to the brain. Keywords: hydrothermal synthesis, nitrogen-doped carbon dots, bioimagingLu SSGuo SSXu PXLi XRZhao YMGu WXue MDove Medical Pressarticlehydrothermal synthesisnitrogen-doped carbon dotsbioimagingblood brain barrierMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6325-6336 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrothermal synthesis nitrogen-doped carbon dots bioimaging blood brain barrier Medicine (General) R5-920
spellingShingle	hydrothermal synthesis nitrogen-doped carbon dots bioimaging blood brain barrier Medicine (General) R5-920 Lu SS Guo SS Xu PX Li XR Zhao YM Gu W Xue M Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
description	Shousi Lu,1,2 Shanshan Guo,1 Pingxiang Xu,1 Xiaorong Li,1 Yuming Zhao,1 Wei Gu,3 Ming Xue1 1Department of Pharmacology, Beijing Laboratory for Biomedical Detection Technology and Instrument, School of Basic Medical Sciences, Capital Medical University, 2China Rehabilitation Research Center, 3Department of Chemistry and Biology, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, China Abstract: Nitrogen-doped carbon dots (N-CDs) were synthesized using a one-pot hydrothermal treatment with citric acid in the presence of polyethylenimine. Transmission electron microscopy analysis revealed that the N-CDs were monodispersed and quasi-spherical with an average size of ~2.6 nm. Under ultraviolet irradiation the N-CDs emitted a strong blue luminescence with a quantum yield as high as 51%. Moreover, the N-CDs exhibited a negligible cytotoxicity and could be applied as efficient nanoprobes for real-time imaging of live cells. In addition, the ability of the N-CDs to cross the blood–brain barrier (BBB) in a concentration-dependent manner was demonstrated using an in vitro BBB model. Therefore, these PEI-passivated N-CDs with real-time live-cell imaging and BBB-penetration capabilities hold promise for traceable drug delivery to the brain. Keywords: hydrothermal synthesis, nitrogen-doped carbon dots, bioimaging
format	article
author	Lu SS Guo SS Xu PX Li XR Zhao YM Gu W Xue M
author_facet	Lu SS Guo SS Xu PX Li XR Zhao YM Gu W Xue M
author_sort	Lu SS
title	Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
title_short	Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
title_full	Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
title_fullStr	Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
title_full_unstemmed	Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
title_sort	hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/39c01391d494426aad9c1a358b691f1c
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Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood&ndash;brain barrier penetration capabilities

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Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities