Connections between prolactin and ovarian cancer.

Ovarian cancer (OC) is characterized by a high morbidity and mortality, highlighting a great need for a better understanding of biological mechanisms that affect OC progression and improving its early detection methods. This study investigates effects of prolactin (PRL) on ovarian cancer cells, anal...

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Autores principales:	Amira Alkharusi, Abdullah AlMuslahi, Najwa AlBalushi, Radiya AlAjmi, Sami AlRawahi, Asmaa AlFarqani, Gunnar Norstedt, Fahad Zadjali
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39c0209eb25c46528f78e7d818a8aa93
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spelling	oai:doaj.org-article:39c0209eb25c46528f78e7d818a8aa932021-12-02T20:15:08ZConnections between prolactin and ovarian cancer.1932-620310.1371/journal.pone.0255701https://doaj.org/article/39c0209eb25c46528f78e7d818a8aa932021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255701https://doaj.org/toc/1932-6203Ovarian cancer (OC) is characterized by a high morbidity and mortality, highlighting a great need for a better understanding of biological mechanisms that affect OC progression and improving its early detection methods. This study investigates effects of prolactin (PRL) on ovarian cancer cells, analyzes PRL receptors (PRLR) in tissue micro arrays and relates PRLR expression to survival of ovarian cancer. A database, composed of transcript profiles from OC, was searched for PRLR expression and results were put in relation to survival. Expression of PRLR in OC tissue sections and OC cell lines SKOV3, OV2008 and OVSAHO was assessed using immunohistochemistry, western blots and quantitative real-time PCR. The biological function of PRLR was evaluated by proliferation, colony formation and wound healing assays. Levels of PRLR mRNA are related to survival; in epithelial OC a high PRLR mRNA expression is related to a shorter survival. Analysis of a tissue micro array consisting of 84 OC showed that 72% were positive for PRLR immuno-staining. PRLR staining tended to be higher in OC of high grade tumors compared to lower grades. PRLR mRNA and protein can further be detected in OC cell lines. Moreover, in vitro treatment with PRL significantly activated the JAK/STAT pathway. PRLR expression is associated with OC survivals. PRL and its receptor may play an onco-modulatory role and promote tumor aggressiveness in OC. Alternatively, increased PRLR levels may form a base for the development of PRLR antagonist or PRLR antagonist-drug conjugate to increase selective uptake of anti-cancer drugs.Amira AlkharusiAbdullah AlMuslahiNajwa AlBalushiRadiya AlAjmiSami AlRawahiAsmaa AlFarqaniGunnar NorstedtFahad ZadjaliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255701 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amira Alkharusi Abdullah AlMuslahi Najwa AlBalushi Radiya AlAjmi Sami AlRawahi Asmaa AlFarqani Gunnar Norstedt Fahad Zadjali Connections between prolactin and ovarian cancer.
description	Ovarian cancer (OC) is characterized by a high morbidity and mortality, highlighting a great need for a better understanding of biological mechanisms that affect OC progression and improving its early detection methods. This study investigates effects of prolactin (PRL) on ovarian cancer cells, analyzes PRL receptors (PRLR) in tissue micro arrays and relates PRLR expression to survival of ovarian cancer. A database, composed of transcript profiles from OC, was searched for PRLR expression and results were put in relation to survival. Expression of PRLR in OC tissue sections and OC cell lines SKOV3, OV2008 and OVSAHO was assessed using immunohistochemistry, western blots and quantitative real-time PCR. The biological function of PRLR was evaluated by proliferation, colony formation and wound healing assays. Levels of PRLR mRNA are related to survival; in epithelial OC a high PRLR mRNA expression is related to a shorter survival. Analysis of a tissue micro array consisting of 84 OC showed that 72% were positive for PRLR immuno-staining. PRLR staining tended to be higher in OC of high grade tumors compared to lower grades. PRLR mRNA and protein can further be detected in OC cell lines. Moreover, in vitro treatment with PRL significantly activated the JAK/STAT pathway. PRLR expression is associated with OC survivals. PRL and its receptor may play an onco-modulatory role and promote tumor aggressiveness in OC. Alternatively, increased PRLR levels may form a base for the development of PRLR antagonist or PRLR antagonist-drug conjugate to increase selective uptake of anti-cancer drugs.
format	article
author	Amira Alkharusi Abdullah AlMuslahi Najwa AlBalushi Radiya AlAjmi Sami AlRawahi Asmaa AlFarqani Gunnar Norstedt Fahad Zadjali
author_facet	Amira Alkharusi Abdullah AlMuslahi Najwa AlBalushi Radiya AlAjmi Sami AlRawahi Asmaa AlFarqani Gunnar Norstedt Fahad Zadjali
author_sort	Amira Alkharusi
title	Connections between prolactin and ovarian cancer.
title_short	Connections between prolactin and ovarian cancer.
title_full	Connections between prolactin and ovarian cancer.
title_fullStr	Connections between prolactin and ovarian cancer.
title_full_unstemmed	Connections between prolactin and ovarian cancer.
title_sort	connections between prolactin and ovarian cancer.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/39c0209eb25c46528f78e7d818a8aa93
work_keys_str_mv	AT amiraalkharusi connectionsbetweenprolactinandovariancancer AT abdullahalmuslahi connectionsbetweenprolactinandovariancancer AT najwaalbalushi connectionsbetweenprolactinandovariancancer AT radiyaalajmi connectionsbetweenprolactinandovariancancer AT samialrawahi connectionsbetweenprolactinandovariancancer AT asmaaalfarqani connectionsbetweenprolactinandovariancancer AT gunnarnorstedt connectionsbetweenprolactinandovariancancer AT fahadzadjali connectionsbetweenprolactinandovariancancer
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Connections between prolactin and ovarian cancer.

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