Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction

The normal function of the airway epithelium is vital for the host’s well-being. Conditions that might compromise the structure and functionality of the airway epithelium include congenital tracheal anomalies, infection, trauma and post-intubation injuries. Recently, the onset of COVID-19 and its co...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohd Heikal Mohd Yunus, Zahra Rashidbenam, Mh Busra Fauzi, Ruszymah Bt Hj Idrus, Aminuddin Bin Saim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	respiratory epithelium polymerized-human plasma respiratory epithelial construct tracheal mucosal reconstruction Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/39c481be81534412ae6cfd43b94e8a4a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:39c481be81534412ae6cfd43b94e8a4a
record_format	dspace
spelling	oai:doaj.org-article:39c481be81534412ae6cfd43b94e8a4a2021-11-11T18:39:55ZEvaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction10.3390/molecules262167241420-3049https://doaj.org/article/39c481be81534412ae6cfd43b94e8a4a2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6724https://doaj.org/toc/1420-3049The normal function of the airway epithelium is vital for the host’s well-being. Conditions that might compromise the structure and functionality of the airway epithelium include congenital tracheal anomalies, infection, trauma and post-intubation injuries. Recently, the onset of COVID-19 and its complications in managing respiratory failure further intensified the need for tracheal tissue replacement. Thus far, plenty of naturally derived, synthetic or allogeneic materials have been studied for their applicability in tracheal tissue replacement. However, a reliable tracheal replacement material is missing. Therefore, this study used a tissue engineering approach for constructing tracheal tissue. Human respiratory epithelial cells (RECs) were isolated from nasal turbinate, and the cells were incorporated into a calcium chloride-polymerized human blood plasma to form a human tissue respiratory epithelial construct (HTREC). The quality of HTREC in vitro, focusing on the cellular proliferation, differentiation and distribution of the RECs, was examined using histological, gene expression and immunocytochemical analysis. Histological analysis showed a homogenous distribution of RECs within the HTREC, with increased proliferation of the residing RECs within 4 days of investigation. Gene expression analysis revealed a significant increase (<i>p</i> < 0.05) in gene expression level of proliferative and respiratory epithelial-specific markers Ki67 and MUC5B, respectively, within 4 days of investigation. Immunohistochemical analysis also confirmed the expression of Ki67 and MUC5AC markers in residing RECs within the HTREC. The findings show that calcium chloride-polymerized human blood plasma is a suitable material, which supports viability, proliferation and mucin secreting phenotype of RECs, and this suggests that HTREC can be a potential candidate for respiratory epithelial tissue reconstruction.Mohd Heikal Mohd YunusZahra RashidbenamMh Busra FauziRuszymah Bt Hj IdrusAminuddin Bin SaimMDPI AGarticlerespiratory epitheliumpolymerized-human plasmarespiratory epithelial construct tracheal mucosal reconstructionOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6724, p 6724 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	respiratory epithelium polymerized-human plasma respiratory epithelial construct tracheal mucosal reconstruction Organic chemistry QD241-441
spellingShingle	respiratory epithelium polymerized-human plasma respiratory epithelial construct tracheal mucosal reconstruction Organic chemistry QD241-441 Mohd Heikal Mohd Yunus Zahra Rashidbenam Mh Busra Fauzi Ruszymah Bt Hj Idrus Aminuddin Bin Saim Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction
description	The normal function of the airway epithelium is vital for the host’s well-being. Conditions that might compromise the structure and functionality of the airway epithelium include congenital tracheal anomalies, infection, trauma and post-intubation injuries. Recently, the onset of COVID-19 and its complications in managing respiratory failure further intensified the need for tracheal tissue replacement. Thus far, plenty of naturally derived, synthetic or allogeneic materials have been studied for their applicability in tracheal tissue replacement. However, a reliable tracheal replacement material is missing. Therefore, this study used a tissue engineering approach for constructing tracheal tissue. Human respiratory epithelial cells (RECs) were isolated from nasal turbinate, and the cells were incorporated into a calcium chloride-polymerized human blood plasma to form a human tissue respiratory epithelial construct (HTREC). The quality of HTREC in vitro, focusing on the cellular proliferation, differentiation and distribution of the RECs, was examined using histological, gene expression and immunocytochemical analysis. Histological analysis showed a homogenous distribution of RECs within the HTREC, with increased proliferation of the residing RECs within 4 days of investigation. Gene expression analysis revealed a significant increase (<i>p</i> < 0.05) in gene expression level of proliferative and respiratory epithelial-specific markers Ki67 and MUC5B, respectively, within 4 days of investigation. Immunohistochemical analysis also confirmed the expression of Ki67 and MUC5AC markers in residing RECs within the HTREC. The findings show that calcium chloride-polymerized human blood plasma is a suitable material, which supports viability, proliferation and mucin secreting phenotype of RECs, and this suggests that HTREC can be a potential candidate for respiratory epithelial tissue reconstruction.
format	article
author	Mohd Heikal Mohd Yunus Zahra Rashidbenam Mh Busra Fauzi Ruszymah Bt Hj Idrus Aminuddin Bin Saim
author_facet	Mohd Heikal Mohd Yunus Zahra Rashidbenam Mh Busra Fauzi Ruszymah Bt Hj Idrus Aminuddin Bin Saim
author_sort	Mohd Heikal Mohd Yunus
title	Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction
title_short	Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction
title_full	Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction
title_fullStr	Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction
title_full_unstemmed	Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction
title_sort	evaluating feasibility of human tissue engineered respiratory epithelium construct as a potential model for tracheal mucosal reconstruction
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/39c481be81534412ae6cfd43b94e8a4a
work_keys_str_mv	AT mohdheikalmohdyunus evaluatingfeasibilityofhumantissueengineeredrespiratoryepitheliumconstructasapotentialmodelfortrachealmucosalreconstruction AT zahrarashidbenam evaluatingfeasibilityofhumantissueengineeredrespiratoryepitheliumconstructasapotentialmodelfortrachealmucosalreconstruction AT mhbusrafauzi evaluatingfeasibilityofhumantissueengineeredrespiratoryepitheliumconstructasapotentialmodelfortrachealmucosalreconstruction AT ruszymahbthjidrus evaluatingfeasibilityofhumantissueengineeredrespiratoryepitheliumconstructasapotentialmodelfortrachealmucosalreconstruction AT aminuddinbinsaim evaluatingfeasibilityofhumantissueengineeredrespiratoryepitheliumconstructasapotentialmodelfortrachealmucosalreconstruction
_version_	1718431755774459904

Evaluating Feasibility of Human Tissue Engineered Respiratory Epithelium Construct as a Potential Model for Tracheal Mucosal Reconstruction

Ejemplares similares