Investigating an increase in Florida manatee mortalities using a proteomic approach

Abstract Two large-scale Florida manatee (Trichechus manatus latirostris) mortality episodes were reported on separate coasts of Florida in 2013. The east coast mortality episode was associated with an unknown etiology in the Indian River Lagoon (IRL). The west coast mortality episode was attributed...

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Autores principales:	Rebecca Lazensky, Cecilia Silva-Sanchez, Kevin J. Kroll, Marjorie Chow, Sixue Chen, Katie Tripp, Michael T. Walsh, Nancy D. Denslow
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39cafdf6ff6349caab61669cea7ec7a8
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spelling	oai:doaj.org-article:39cafdf6ff6349caab61669cea7ec7a82021-12-02T14:03:57ZInvestigating an increase in Florida manatee mortalities using a proteomic approach10.1038/s41598-021-83687-y2045-2322https://doaj.org/article/39cafdf6ff6349caab61669cea7ec7a82021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83687-yhttps://doaj.org/toc/2045-2322Abstract Two large-scale Florida manatee (Trichechus manatus latirostris) mortality episodes were reported on separate coasts of Florida in 2013. The east coast mortality episode was associated with an unknown etiology in the Indian River Lagoon (IRL). The west coast mortality episode was attributed to a persistent Karenia brevis algal bloom or ‘red tide’ centered in Southwest Florida. Manatees from the IRL also had signs of cold stress. To investigate these two mortality episodes, two proteomic experiments were performed, using two-dimensional difference in gel electrophoresis (2D-DIGE) and isobaric tags for relative and absolute quantification (iTRAQ) LC–MS/MS. Manatees from the IRL displayed increased levels of several proteins in their serum samples compared to controls, including kininogen-1 isoform 1, alpha-1-microglobulin/bikunen precursor, histidine-rich glycoprotein, properdin, and complement C4-A isoform 1. In the red tide group, the following proteins were increased: ceruloplasmin, pyruvate kinase isozymes M1/M2 isoform 3, angiotensinogen, complement C4-A isoform 1, and complement C3. These proteins are associated with acute-phase response, amyloid formation and accumulation, copper and iron homeostasis, the complement cascade pathway, and other important cellular functions. The increased level of complement C4 protein observed in the red tide group was confirmed through the use of Western Blot.Rebecca LazenskyCecilia Silva-SanchezKevin J. KrollMarjorie ChowSixue ChenKatie TrippMichael T. WalshNancy D. DenslowNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rebecca Lazensky Cecilia Silva-Sanchez Kevin J. Kroll Marjorie Chow Sixue Chen Katie Tripp Michael T. Walsh Nancy D. Denslow Investigating an increase in Florida manatee mortalities using a proteomic approach
description	Abstract Two large-scale Florida manatee (Trichechus manatus latirostris) mortality episodes were reported on separate coasts of Florida in 2013. The east coast mortality episode was associated with an unknown etiology in the Indian River Lagoon (IRL). The west coast mortality episode was attributed to a persistent Karenia brevis algal bloom or ‘red tide’ centered in Southwest Florida. Manatees from the IRL also had signs of cold stress. To investigate these two mortality episodes, two proteomic experiments were performed, using two-dimensional difference in gel electrophoresis (2D-DIGE) and isobaric tags for relative and absolute quantification (iTRAQ) LC–MS/MS. Manatees from the IRL displayed increased levels of several proteins in their serum samples compared to controls, including kininogen-1 isoform 1, alpha-1-microglobulin/bikunen precursor, histidine-rich glycoprotein, properdin, and complement C4-A isoform 1. In the red tide group, the following proteins were increased: ceruloplasmin, pyruvate kinase isozymes M1/M2 isoform 3, angiotensinogen, complement C4-A isoform 1, and complement C3. These proteins are associated with acute-phase response, amyloid formation and accumulation, copper and iron homeostasis, the complement cascade pathway, and other important cellular functions. The increased level of complement C4 protein observed in the red tide group was confirmed through the use of Western Blot.
format	article
author	Rebecca Lazensky Cecilia Silva-Sanchez Kevin J. Kroll Marjorie Chow Sixue Chen Katie Tripp Michael T. Walsh Nancy D. Denslow
author_facet	Rebecca Lazensky Cecilia Silva-Sanchez Kevin J. Kroll Marjorie Chow Sixue Chen Katie Tripp Michael T. Walsh Nancy D. Denslow
author_sort	Rebecca Lazensky
title	Investigating an increase in Florida manatee mortalities using a proteomic approach
title_short	Investigating an increase in Florida manatee mortalities using a proteomic approach
title_full	Investigating an increase in Florida manatee mortalities using a proteomic approach
title_fullStr	Investigating an increase in Florida manatee mortalities using a proteomic approach
title_full_unstemmed	Investigating an increase in Florida manatee mortalities using a proteomic approach
title_sort	investigating an increase in florida manatee mortalities using a proteomic approach
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/39cafdf6ff6349caab61669cea7ec7a8
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Investigating an increase in Florida manatee mortalities using a proteomic approach

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