Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types

Mandy Brueckner,1 Steffen Jankuhn,2,3 Eva-Maria Jülke,1 Uta Reibetanz1 1Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, Germany; 2Division of Nuclear Solid State Physics, Faculty of Physics and Geosciences, University of Leipzig, Leipzig, Germ...

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Autores principales:	Brueckner M, Jankuhn S, Jülke EM, Reibetanz U
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Layer-by-Layer (LbL) uptake cell viability microparticles microcapsules material properties Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/39cb7ff3ac5a4b0182e07b85070abce5
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spelling	oai:doaj.org-article:39cb7ff3ac5a4b0182e07b85070abce52021-12-02T01:56:40ZCellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types1178-2013https://doaj.org/article/39cb7ff3ac5a4b0182e07b85070abce52018-04-01T00:00:00Zhttps://www.dovepress.com/cellular-interaction-of-a-layer-by-layer-based-drug-delivery-system-de-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mandy Brueckner,1 Steffen Jankuhn,2,3 Eva-Maria Jülke,1 Uta Reibetanz1 1Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, Germany; 2Division of Nuclear Solid State Physics, Faculty of Physics and Geosciences, University of Leipzig, Leipzig, Germany; 3Office for Environmental Protection and Occupational Safety, University of Leipzig, Leipzig, Germany Background: Drug delivery systems (DDS) and their interaction with cells are a controversial topic in the development of therapeutic concepts and approaches. On one hand, DDS are very useful for protected and targeted transport of defined dosages of active agents. On the other hand, their physicochemical properties such as material, size, shape, charge, or stiffness have a huge impact on cellular uptake and intracellular processing. Additionally, even identical DDS can undergo a completely diverse interaction with different cell types. However, quite often in in vitro DDS/cell interaction experiments, those aspects are not considered and DDS and cells are randomly chosen. Methods and results: Hence, our investigations provide an insight into layer-by-layer designed microcarriers with modifications of only some of the most important parameters (surface charge, stiffness, and applied microcarrier/cell ratio) and their influence on cellular uptake and viability. We also considered the interaction of these differently equipped DDS with several cell types and investigated professional phagocytes (neutrophil granulocytes; macrophages) as well as non-professional phagocytes (epithelial cells) under comparable conditions. We found that even small modifications such as layer-by-layer (LbL)-microcarriers with positive or negative surface charge, or LbL-microcarriers with solid core or as hollow capsules but equipped with the same surface properties, show significant differences in interaction and viability, and several cell types react very differently to the offered DDS. Conclusion: As a consequence, the properties of the DDS have to be carefully chosen with respect to the addressed cell type with the aim to efficiently transport a desired agent. Keywords: layer-by-layer, LbL, uptake, cell viability, microparticles, microcapsules, material propertiesBrueckner MJankuhn SJülke EMReibetanz UDove Medical PressarticleLayer-by-Layer (LbL)uptakecell viabilitymicroparticlesmicrocapsulesmaterial propertiesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2079-2091 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Layer-by-Layer (LbL) uptake cell viability microparticles microcapsules material properties Medicine (General) R5-920
spellingShingle	Layer-by-Layer (LbL) uptake cell viability microparticles microcapsules material properties Medicine (General) R5-920 Brueckner M Jankuhn S Jülke EM Reibetanz U Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
description	Mandy Brueckner,1 Steffen Jankuhn,2,3 Eva-Maria Jülke,1 Uta Reibetanz1 1Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, Germany; 2Division of Nuclear Solid State Physics, Faculty of Physics and Geosciences, University of Leipzig, Leipzig, Germany; 3Office for Environmental Protection and Occupational Safety, University of Leipzig, Leipzig, Germany Background: Drug delivery systems (DDS) and their interaction with cells are a controversial topic in the development of therapeutic concepts and approaches. On one hand, DDS are very useful for protected and targeted transport of defined dosages of active agents. On the other hand, their physicochemical properties such as material, size, shape, charge, or stiffness have a huge impact on cellular uptake and intracellular processing. Additionally, even identical DDS can undergo a completely diverse interaction with different cell types. However, quite often in in vitro DDS/cell interaction experiments, those aspects are not considered and DDS and cells are randomly chosen. Methods and results: Hence, our investigations provide an insight into layer-by-layer designed microcarriers with modifications of only some of the most important parameters (surface charge, stiffness, and applied microcarrier/cell ratio) and their influence on cellular uptake and viability. We also considered the interaction of these differently equipped DDS with several cell types and investigated professional phagocytes (neutrophil granulocytes; macrophages) as well as non-professional phagocytes (epithelial cells) under comparable conditions. We found that even small modifications such as layer-by-layer (LbL)-microcarriers with positive or negative surface charge, or LbL-microcarriers with solid core or as hollow capsules but equipped with the same surface properties, show significant differences in interaction and viability, and several cell types react very differently to the offered DDS. Conclusion: As a consequence, the properties of the DDS have to be carefully chosen with respect to the addressed cell type with the aim to efficiently transport a desired agent. Keywords: layer-by-layer, LbL, uptake, cell viability, microparticles, microcapsules, material properties
format	article
author	Brueckner M Jankuhn S Jülke EM Reibetanz U
author_facet	Brueckner M Jankuhn S Jülke EM Reibetanz U
author_sort	Brueckner M
title	Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_short	Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_full	Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_fullStr	Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_full_unstemmed	Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
title_sort	cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/39cb7ff3ac5a4b0182e07b85070abce5
work_keys_str_mv	AT bruecknerm cellularinteractionofalayerbylayerbaseddrugdeliverysystemdependingonmaterialpropertiesandcelltypes AT jankuhns cellularinteractionofalayerbylayerbaseddrugdeliverysystemdependingonmaterialpropertiesandcelltypes AT julkeem cellularinteractionofalayerbylayerbaseddrugdeliverysystemdependingonmaterialpropertiesandcelltypes AT reibetanzu cellularinteractionofalayerbylayerbaseddrugdeliverysystemdependingonmaterialpropertiesandcelltypes
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Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types

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