Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>
In some Gram-negative bacteria, <i>ompF</i> encodes outer membrane protein F (OmpF), which is a cation-selective porin and is responsible for the passive transport of small molecules across the outer membrane. However, there are few reports about the functions of this gene in <i>Cr...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/39cfe98ffdb4496bbbd40c0b97ffeb58 |
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| Sumario: | In some Gram-negative bacteria, <i>ompF</i> encodes outer membrane protein F (OmpF), which is a cation-selective porin and is responsible for the passive transport of small molecules across the outer membrane. However, there are few reports about the functions of this gene in <i>Cronobacter sakazakii</i>. To investigate the role of <i>ompF</i> in detail, an <i>ompF</i> disruption strain (Δ<i>ompF</i>) and a complementation strain (cp<i>ompF</i>) were successfully obtained. We find that OmpF can affect the ability of biofilm formation in <i>C. sakazakii</i>. In addition, the variations in biofilm composition of <i>C. sakazakii</i> were examined using Raman spectroscopy analyses caused by knocking out <i>ompF</i>, and the result indicated that the levels of certain biofilm components, including lipopolysaccharide (LPS), were significantly decreased in the mutant (Δ<i>ompF</i>). Then, SDS-PAGE was used to further analyze the LPS content, and the result showed that the LPS levels were significantly reduced in the absence of <i>ompF</i>. Therefore, we conclude that OmpF affects biofilm formation in <i>C. sakazakii</i> by reducing the amount of LPS. Furthermore, the Δ<i>ompF</i> mutant showed decreased (2.7-fold) adhesion to and invasion of HCT-8 cells. In an antibiotic susceptibility analysis, the Δ<i>ompF</i> mutant showed significantly smaller inhibition zones than the WT, indicating that OmpF had a positive effect on the influx of antibiotics into the cells. In summary, <i>ompF</i> plays a positive regulatory role in the biofilm formation and adhesion/invasion, which is achieved by regulating the amount of LPS, but is a negative regulator of antibiotic resistance in <i>C. sakazakii</i>. |
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