Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in Cronobacter sakazakii

In some Gram-negative bacteria, ompF encodes outer membrane protein F (OmpF), which is a cation-selective porin and is responsible for the passive transport of small molecules across the outer membrane. However, there are few reports about the functions of this gene in Cr...

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Autores principales:	Jianxin Gao, Zhonghui Han, Ping Li, Hongyan Zhang, Xinjun Du, Shuo Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>ompF</i> <i>Cronobacter sakazakii</i> LPS biofilm formation adhesion/invasion Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/39cfe98ffdb4496bbbd40c0b97ffeb58
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spelling	oai:doaj.org-article:39cfe98ffdb4496bbbd40c0b97ffeb582021-11-25T18:25:14ZOuter Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>10.3390/microorganisms91123382076-2607https://doaj.org/article/39cfe98ffdb4496bbbd40c0b97ffeb582021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2338https://doaj.org/toc/2076-2607In some Gram-negative bacteria, <i>ompF</i> encodes outer membrane protein F (OmpF), which is a cation-selective porin and is responsible for the passive transport of small molecules across the outer membrane. However, there are few reports about the functions of this gene in <i>Cronobacter sakazakii</i>. To investigate the role of <i>ompF</i> in detail, an <i>ompF</i> disruption strain (Δ<i>ompF</i>) and a complementation strain (cp<i>ompF</i>) were successfully obtained. We find that OmpF can affect the ability of biofilm formation in <i>C. sakazakii</i>. In addition, the variations in biofilm composition of <i>C. sakazakii</i> were examined using Raman spectroscopy analyses caused by knocking out <i>ompF</i>, and the result indicated that the levels of certain biofilm components, including lipopolysaccharide (LPS), were significantly decreased in the mutant (Δ<i>ompF</i>). Then, SDS-PAGE was used to further analyze the LPS content, and the result showed that the LPS levels were significantly reduced in the absence of <i>ompF</i>. Therefore, we conclude that OmpF affects biofilm formation in <i>C. sakazakii</i> by reducing the amount of LPS. Furthermore, the Δ<i>ompF</i> mutant showed decreased (2.7-fold) adhesion to and invasion of HCT-8 cells. In an antibiotic susceptibility analysis, the Δ<i>ompF</i> mutant showed significantly smaller inhibition zones than the WT, indicating that OmpF had a positive effect on the influx of antibiotics into the cells. In summary, <i>ompF</i> plays a positive regulatory role in the biofilm formation and adhesion/invasion, which is achieved by regulating the amount of LPS, but is a negative regulator of antibiotic resistance in <i>C. sakazakii</i>.Jianxin GaoZhonghui HanPing LiHongyan ZhangXinjun DuShuo WangMDPI AGarticle<i>ompF</i><i>Cronobacter sakazakii</i>LPSbiofilm formationadhesion/invasionBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2338, p 2338 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>ompF</i> <i>Cronobacter sakazakii</i> LPS biofilm formation adhesion/invasion Biology (General) QH301-705.5
spellingShingle	<i>ompF</i> <i>Cronobacter sakazakii</i> LPS biofilm formation adhesion/invasion Biology (General) QH301-705.5 Jianxin Gao Zhonghui Han Ping Li Hongyan Zhang Xinjun Du Shuo Wang Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>
description	In some Gram-negative bacteria, <i>ompF</i> encodes outer membrane protein F (OmpF), which is a cation-selective porin and is responsible for the passive transport of small molecules across the outer membrane. However, there are few reports about the functions of this gene in <i>Cronobacter sakazakii</i>. To investigate the role of <i>ompF</i> in detail, an <i>ompF</i> disruption strain (Δ<i>ompF</i>) and a complementation strain (cp<i>ompF</i>) were successfully obtained. We find that OmpF can affect the ability of biofilm formation in <i>C. sakazakii</i>. In addition, the variations in biofilm composition of <i>C. sakazakii</i> were examined using Raman spectroscopy analyses caused by knocking out <i>ompF</i>, and the result indicated that the levels of certain biofilm components, including lipopolysaccharide (LPS), were significantly decreased in the mutant (Δ<i>ompF</i>). Then, SDS-PAGE was used to further analyze the LPS content, and the result showed that the LPS levels were significantly reduced in the absence of <i>ompF</i>. Therefore, we conclude that OmpF affects biofilm formation in <i>C. sakazakii</i> by reducing the amount of LPS. Furthermore, the Δ<i>ompF</i> mutant showed decreased (2.7-fold) adhesion to and invasion of HCT-8 cells. In an antibiotic susceptibility analysis, the Δ<i>ompF</i> mutant showed significantly smaller inhibition zones than the WT, indicating that OmpF had a positive effect on the influx of antibiotics into the cells. In summary, <i>ompF</i> plays a positive regulatory role in the biofilm formation and adhesion/invasion, which is achieved by regulating the amount of LPS, but is a negative regulator of antibiotic resistance in <i>C. sakazakii</i>.
format	article
author	Jianxin Gao Zhonghui Han Ping Li Hongyan Zhang Xinjun Du Shuo Wang
author_facet	Jianxin Gao Zhonghui Han Ping Li Hongyan Zhang Xinjun Du Shuo Wang
author_sort	Jianxin Gao
title	Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>
title_short	Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>
title_full	Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>
title_fullStr	Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>
title_full_unstemmed	Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>
title_sort	outer membrane protein f is involved in biofilm formation, virulence and antibiotic resistance in <i>cronobacter sakazakii</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/39cfe98ffdb4496bbbd40c0b97ffeb58
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Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance in <i>Cronobacter sakazakii</i>

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