Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>

ABSTRACT The vast majority of annotated transcripts in bacteria are mRNAs. Here we identify ~1,000 antisense transcripts in the model bacterium Escherichia coli. We propose that these transcripts are generated by promiscuous transcription initiation within genes and that many of them regulate expres...

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Autores principales:	James E. Dornenburg, Anne M. DeVita, Michael J. Palumbo, Joseph T. Wade
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/39d6736279744c988881d8bc48371103
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spelling	oai:doaj.org-article:39d6736279744c988881d8bc483711032021-11-15T15:38:14ZWidespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>10.1128/mBio.00024-102150-7511https://doaj.org/article/39d6736279744c988881d8bc483711032010-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00024-10https://doaj.org/toc/2150-7511ABSTRACT The vast majority of annotated transcripts in bacteria are mRNAs. Here we identify ~1,000 antisense transcripts in the model bacterium Escherichia coli. We propose that these transcripts are generated by promiscuous transcription initiation within genes and that many of them regulate expression of the overlapping gene. IMPORTANCE The vast majority of known genes in bacteria are protein coding, and there are very few known antisense transcripts within these genes, i.e., RNAs that are encoded opposite the gene. Here we demonstrate the existence of ~1,000 antisense RNAs in the model bacterium Escherichia coli. Given the high potential for these RNAs to base pair with mRNA of the overlapping gene and the likelihood of clashes between transcription complexes of antisense and sense transcripts, we propose that antisense RNAs represent an important but overlooked class of regulatory molecule.James E. DornenburgAnne M. DeVitaMichael J. PalumboJoseph T. WadeAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 1, Iss 1 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 James E. Dornenburg Anne M. DeVita Michael J. Palumbo Joseph T. Wade Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>
description	ABSTRACT The vast majority of annotated transcripts in bacteria are mRNAs. Here we identify ~1,000 antisense transcripts in the model bacterium Escherichia coli. We propose that these transcripts are generated by promiscuous transcription initiation within genes and that many of them regulate expression of the overlapping gene. IMPORTANCE The vast majority of known genes in bacteria are protein coding, and there are very few known antisense transcripts within these genes, i.e., RNAs that are encoded opposite the gene. Here we demonstrate the existence of ~1,000 antisense RNAs in the model bacterium Escherichia coli. Given the high potential for these RNAs to base pair with mRNA of the overlapping gene and the likelihood of clashes between transcription complexes of antisense and sense transcripts, we propose that antisense RNAs represent an important but overlooked class of regulatory molecule.
format	article
author	James E. Dornenburg Anne M. DeVita Michael J. Palumbo Joseph T. Wade
author_facet	James E. Dornenburg Anne M. DeVita Michael J. Palumbo Joseph T. Wade
author_sort	James E. Dornenburg
title	Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>
title_short	Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full	Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>
title_fullStr	Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full_unstemmed	Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>
title_sort	widespread antisense transcription in <named-content content-type="genus-species">escherichia coli</named-content>
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/39d6736279744c988881d8bc48371103
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Widespread Antisense Transcription in <named-content content-type="genus-species">Escherichia coli</named-content>

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