Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid

Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid

Farahnaz Barahuie,1 Mohd Zobir Hussein,1 Shafinaz Abd Gani,2,3 Sharida Fakurazi,3,4 Zulkarnain Zainal11Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Scie...

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Autores principales: Barahuie F, Hussein MZ, Abd Gani S, Fakurazi S, Zainal Z
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/39d7cad72b3a4db39d8fa12063bb9e0a
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spelling oai:doaj.org-article:39d7cad72b3a4db39d8fa12063bb9e0a2021-12-02T07:31:58ZAnticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid1178-2013https://doaj.org/article/39d7cad72b3a4db39d8fa12063bb9e0a2014-06-01T00:00:00Zhttp://www.dovepress.com/anticancer-nanodelivery-system-with-controlled-release-property-based--a17391https://doaj.org/toc/1178-2013 Farahnaz Barahuie,1 Mohd Zobir Hussein,1 Shafinaz Abd Gani,2,3 Sharida Fakurazi,3,4 Zulkarnain Zainal11Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 3Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 4Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaBackground: We characterize a novel nanocomposite that acts as an efficient anticancer agent. Methods: This nanocomposite consists of zinc layered hydroxide intercalated with protocatechuate (an anionic form of protocatechuic acid), that has been synthesized using a direct method with zinc oxide and protocatechuic acid as precursors.Results: The resulting protocatechuic acid nanocomposite (PAN) showed a basal spacing of 12.7 Å, indicating that protocatechuate was intercalated in a monolayer arrangement, with an angle of 54° from the Z-axis between the interlayers of the zinc layered hydroxide, and an estimated drug loading of about 35.7%. PAN exhibited the properties of a mesoporous type material, with greatly enhanced thermal stability of protocatechuate as compared to its free counterpart. The presence of protocatechuate in the interlayers of the zinc layered hydroxide was further supported by Fourier transform infrared spectroscopy. Protocatechuate was released from PAN in a slow and sustained manner. This mechanism of release was well represented by a pseudo-second order kinetics model. PAN has shown increased cytotoxicity compared to the free form of protocatechuic acid in all cancer cell lines tested. Tumor growth suppression was extensive, particularly in HepG2 and HT29 cell lines. Conclusion: PAN is suitable for use as a controlled release formulation, and our in vitro evidence indicates that PAN is an effective anticancer agent. PAN may have potential as a chemotherapeutic drug for human cancer.Keywords: protocatechuic acid, nanocomposite, zinc layered hydroxide, zinc oxide, 3T3, HepG2, HeLa, HT29Barahuie FHussein MZAbd Gani SFakurazi SZainal ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3137-3149 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Barahuie F
Hussein MZ
Abd Gani S
Fakurazi S
Zainal Z
Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
description Farahnaz Barahuie,1 Mohd Zobir Hussein,1 Shafinaz Abd Gani,2,3 Sharida Fakurazi,3,4 Zulkarnain Zainal11Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 3Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 4Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaBackground: We characterize a novel nanocomposite that acts as an efficient anticancer agent. Methods: This nanocomposite consists of zinc layered hydroxide intercalated with protocatechuate (an anionic form of protocatechuic acid), that has been synthesized using a direct method with zinc oxide and protocatechuic acid as precursors.Results: The resulting protocatechuic acid nanocomposite (PAN) showed a basal spacing of 12.7 Å, indicating that protocatechuate was intercalated in a monolayer arrangement, with an angle of 54° from the Z-axis between the interlayers of the zinc layered hydroxide, and an estimated drug loading of about 35.7%. PAN exhibited the properties of a mesoporous type material, with greatly enhanced thermal stability of protocatechuate as compared to its free counterpart. The presence of protocatechuate in the interlayers of the zinc layered hydroxide was further supported by Fourier transform infrared spectroscopy. Protocatechuate was released from PAN in a slow and sustained manner. This mechanism of release was well represented by a pseudo-second order kinetics model. PAN has shown increased cytotoxicity compared to the free form of protocatechuic acid in all cancer cell lines tested. Tumor growth suppression was extensive, particularly in HepG2 and HT29 cell lines. Conclusion: PAN is suitable for use as a controlled release formulation, and our in vitro evidence indicates that PAN is an effective anticancer agent. PAN may have potential as a chemotherapeutic drug for human cancer.Keywords: protocatechuic acid, nanocomposite, zinc layered hydroxide, zinc oxide, 3T3, HepG2, HeLa, HT29
format article
author Barahuie F
Hussein MZ
Abd Gani S
Fakurazi S
Zainal Z
author_facet Barahuie F
Hussein MZ
Abd Gani S
Fakurazi S
Zainal Z
author_sort Barahuie F
title Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_short Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_full Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_fullStr Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_full_unstemmed Anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
title_sort anticancer nanodelivery system with controlled release property based on protocatechuate–zinc layered hydroxide nanohybrid
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/39d7cad72b3a4db39d8fa12063bb9e0a
work_keys_str_mv AT barahuief anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
AT husseinmz anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
AT abdganis anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
AT fakurazis anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
AT zainalz anticancernanodeliverysystemwithcontrolledreleasepropertybasedonprotocatechuatendashzinclayeredhydroxidenanohybrid
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