Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia

Abstract Post-surgical management is an important issue in veterinary medicine, requiring biomarkers with high sensitivity and specificity for timely and effective treatment. Emerging evidence suggests that miRNAs are promising stress- and pain-related markers. The aims were to profile the circulati...

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Autores principales:	Edoardo Bardi, Stefano Brizzola, Giuliano Ravasio, Stefano Romussi, Paola Dall’Ara, Valentina Zamarian, Maddalena Arigoni, Raffaele Adolfo Calogero, Cristina Lecchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39db6ee905a94a05a61f2ae821220ff9
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spelling	oai:doaj.org-article:39db6ee905a94a05a61f2ae821220ff92021-12-02T17:03:49ZCirculating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia10.1038/s41598-021-94113-82045-2322https://doaj.org/article/39db6ee905a94a05a61f2ae821220ff92021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94113-8https://doaj.org/toc/2045-2322Abstract Post-surgical management is an important issue in veterinary medicine, requiring biomarkers with high sensitivity and specificity for timely and effective treatment. Emerging evidence suggests that miRNAs are promising stress- and pain-related markers. The aims were to profile the circulating miRNA signature in plasma of turtles (Trachemys scripta) and point out potential candidate biomarkers to assess the status of the animal. The plasma of female turtles underwent surgical gonadectomy were collected 24 h pre-surgery, and 2.5 h and 36 h post-surgery. The expression of miRNAs was profiled by Next Generation Sequencing and the dysregulated miRNAs were validated using RT-qPCR. The diagnostic value of miRNAs was calculated by ROC curves. The results showed that 14 miRNAs were differentially expressed over time. RT-qPCR validation highlighted that 2-miR-499-3p and miR-203-5p-out of 8 miRNAs tested were effectively modulated. The Area Under the Curve (AUC) of miR-203-5p was fair (AUC 0.7934) in discriminating pre- and 36 h post-surgery samples and poor for other time points; the AUC of miR-499-3p was excellent (AUC 0.944) in discriminating pre-surgery and 2.5 h post-surgery samples, and fair in discriminating pre-surgery and 36 h post-surgery (AUC 0.7292) and 2.5 h and 36 h post-surgery (AUC 0.7569) samples. In conclusion, we demonstrated for the first time that miRNAs profile changes in plasma of turtles underwent surgical oophorectomy and identified miR-203-5p and miR-499-3p as potential candidate biomarkers to assess animals' status. Further studies are necessary to confirm their diagnostic value and to investigate functional and mechanistic networks to improve our understanding of the biological processes.Edoardo BardiStefano BrizzolaGiuliano RavasioStefano RomussiPaola Dall’AraValentina ZamarianMaddalena ArigoniRaffaele Adolfo CalogeroCristina LecchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Edoardo Bardi Stefano Brizzola Giuliano Ravasio Stefano Romussi Paola Dall’Ara Valentina Zamarian Maddalena Arigoni Raffaele Adolfo Calogero Cristina Lecchi Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia
description	Abstract Post-surgical management is an important issue in veterinary medicine, requiring biomarkers with high sensitivity and specificity for timely and effective treatment. Emerging evidence suggests that miRNAs are promising stress- and pain-related markers. The aims were to profile the circulating miRNA signature in plasma of turtles (Trachemys scripta) and point out potential candidate biomarkers to assess the status of the animal. The plasma of female turtles underwent surgical gonadectomy were collected 24 h pre-surgery, and 2.5 h and 36 h post-surgery. The expression of miRNAs was profiled by Next Generation Sequencing and the dysregulated miRNAs were validated using RT-qPCR. The diagnostic value of miRNAs was calculated by ROC curves. The results showed that 14 miRNAs were differentially expressed over time. RT-qPCR validation highlighted that 2-miR-499-3p and miR-203-5p-out of 8 miRNAs tested were effectively modulated. The Area Under the Curve (AUC) of miR-203-5p was fair (AUC 0.7934) in discriminating pre- and 36 h post-surgery samples and poor for other time points; the AUC of miR-499-3p was excellent (AUC 0.944) in discriminating pre-surgery and 2.5 h post-surgery samples, and fair in discriminating pre-surgery and 36 h post-surgery (AUC 0.7292) and 2.5 h and 36 h post-surgery (AUC 0.7569) samples. In conclusion, we demonstrated for the first time that miRNAs profile changes in plasma of turtles underwent surgical oophorectomy and identified miR-203-5p and miR-499-3p as potential candidate biomarkers to assess animals' status. Further studies are necessary to confirm their diagnostic value and to investigate functional and mechanistic networks to improve our understanding of the biological processes.
format	article
author	Edoardo Bardi Stefano Brizzola Giuliano Ravasio Stefano Romussi Paola Dall’Ara Valentina Zamarian Maddalena Arigoni Raffaele Adolfo Calogero Cristina Lecchi
author_facet	Edoardo Bardi Stefano Brizzola Giuliano Ravasio Stefano Romussi Paola Dall’Ara Valentina Zamarian Maddalena Arigoni Raffaele Adolfo Calogero Cristina Lecchi
author_sort	Edoardo Bardi
title	Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia
title_short	Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia
title_full	Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia
title_fullStr	Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia
title_full_unstemmed	Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia
title_sort	circulating mirnome of trachemys scripta after elective gonadectomy under general anesthesia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/39db6ee905a94a05a61f2ae821220ff9
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Circulating miRNome of Trachemys scripta after elective gonadectomy under general anesthesia

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