Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
Abstract The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collec...
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Nature Portfolio
2021
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oai:doaj.org-article:39dfb0e9ecc74fe6a6c372c4079c88bd2021-12-02T16:26:38ZImpact of repeated nasal sampling on detection and quantification of SARS-CoV-210.1038/s41598-021-94547-02045-2322https://doaj.org/article/39dfb0e9ecc74fe6a6c372c4079c88bd2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94547-0https://doaj.org/toc/2045-2322Abstract The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and test performance among 325 symptomatic patients undergoing COVID-19 testing in Atlanta, GA. High concordance was found between consecutively collected mid-turbinate samples with both molecular (n = 74, 100% concordance) and antigen-based (n = 147, 97% concordance, kappa = 0.95, CI = 0.88–1.00) diagnostic assays. Repeated sample collection does not decrease COVID-19 test performance, demonstrating that multiple samples can be collected for assay validation and clinical diagnosis.Joshua M. LevyJennifer K. FredianiErika A. TyburskiAnna WoodJanet FigueroaRussell R. KempkerPaulina A. RebolledoMark D. GonzalezJulie SullivanMiriam B. VosJared O’NealGreg S. MartinWilbur A. LamJesse J. WaggonerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-5 (2021) |
| institution |
DOAJ |
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DOAJ |
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EN |
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Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Joshua M. Levy Jennifer K. Frediani Erika A. Tyburski Anna Wood Janet Figueroa Russell R. Kempker Paulina A. Rebolledo Mark D. Gonzalez Julie Sullivan Miriam B. Vos Jared O’Neal Greg S. Martin Wilbur A. Lam Jesse J. Waggoner Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| description |
Abstract The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and test performance among 325 symptomatic patients undergoing COVID-19 testing in Atlanta, GA. High concordance was found between consecutively collected mid-turbinate samples with both molecular (n = 74, 100% concordance) and antigen-based (n = 147, 97% concordance, kappa = 0.95, CI = 0.88–1.00) diagnostic assays. Repeated sample collection does not decrease COVID-19 test performance, demonstrating that multiple samples can be collected for assay validation and clinical diagnosis. |
| format |
article |
| author |
Joshua M. Levy Jennifer K. Frediani Erika A. Tyburski Anna Wood Janet Figueroa Russell R. Kempker Paulina A. Rebolledo Mark D. Gonzalez Julie Sullivan Miriam B. Vos Jared O’Neal Greg S. Martin Wilbur A. Lam Jesse J. Waggoner |
| author_facet |
Joshua M. Levy Jennifer K. Frediani Erika A. Tyburski Anna Wood Janet Figueroa Russell R. Kempker Paulina A. Rebolledo Mark D. Gonzalez Julie Sullivan Miriam B. Vos Jared O’Neal Greg S. Martin Wilbur A. Lam Jesse J. Waggoner |
| author_sort |
Joshua M. Levy |
| title |
Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_short |
Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_full |
Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_fullStr |
Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_full_unstemmed |
Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_sort |
impact of repeated nasal sampling on detection and quantification of sars-cov-2 |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/39dfb0e9ecc74fe6a6c372c4079c88bd |
| work_keys_str_mv |
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