Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts

Abstract It has been proposed that girls with adolescent idiopathic scoliosis (AIS) tend to have a taller stature and a lower body mass index. Energy homeostasis, that is known to affect bone growth, could contribute to these characteristics. In circulation, dipeptidyl peptidase-4 (DPP-4) inactivate...

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Autores principales:	Emilie Normand, Anita Franco, Alain Moreau, Valérie Marcil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39ecfbac05f2488ba6eb6d1dbf57f2f4
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spelling	oai:doaj.org-article:39ecfbac05f2488ba6eb6d1dbf57f2f42021-12-02T16:06:30ZDipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts10.1038/s41598-017-03310-x2045-2322https://doaj.org/article/39ecfbac05f2488ba6eb6d1dbf57f2f42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03310-xhttps://doaj.org/toc/2045-2322Abstract It has been proposed that girls with adolescent idiopathic scoliosis (AIS) tend to have a taller stature and a lower body mass index. Energy homeostasis, that is known to affect bone growth, could contribute to these characteristics. In circulation, dipeptidyl peptidase-4 (DPP-4) inactivates glucagon-like peptide-1 (GLP-1), an incretin that promotes insulin secretion and sensitivity. Our objectives were to investigate DPP-4 status in plasma and in osteoblasts of AIS subjects and controls and to evaluate the regulatory role of metabolic effectors on DPP-4 expression. DPP-4 activity was assessed in plasma of 113 girls and 62 age-matched controls. Osteoblasts were isolated from bone specimens of AIS patients and controls. Human cells were incubated with glucose, insulin, GLP-1 and butyrate. Gene and protein expressions were evaluated by RT-qPCR and Western blot. Our results showed 14% inferior plasma DPP-4 activity in AIS patients when compared to healthy controls (P = 0.0357). Similarly, osteoblasts derived from AIS subjects had lower DPP-4 gene and protein expression than controls by 90.5% and 57.1% respectively (P < 0.009). DPP-4 expression was regulated in a different manner in osteoblasts isolated from AIS participants compared to controls. Our results suggest a role for incretins in AIS development and severity.Emilie NormandAnita FrancoAlain MoreauValérie MarcilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emilie Normand Anita Franco Alain Moreau Valérie Marcil Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts
description	Abstract It has been proposed that girls with adolescent idiopathic scoliosis (AIS) tend to have a taller stature and a lower body mass index. Energy homeostasis, that is known to affect bone growth, could contribute to these characteristics. In circulation, dipeptidyl peptidase-4 (DPP-4) inactivates glucagon-like peptide-1 (GLP-1), an incretin that promotes insulin secretion and sensitivity. Our objectives were to investigate DPP-4 status in plasma and in osteoblasts of AIS subjects and controls and to evaluate the regulatory role of metabolic effectors on DPP-4 expression. DPP-4 activity was assessed in plasma of 113 girls and 62 age-matched controls. Osteoblasts were isolated from bone specimens of AIS patients and controls. Human cells were incubated with glucose, insulin, GLP-1 and butyrate. Gene and protein expressions were evaluated by RT-qPCR and Western blot. Our results showed 14% inferior plasma DPP-4 activity in AIS patients when compared to healthy controls (P = 0.0357). Similarly, osteoblasts derived from AIS subjects had lower DPP-4 gene and protein expression than controls by 90.5% and 57.1% respectively (P < 0.009). DPP-4 expression was regulated in a different manner in osteoblasts isolated from AIS participants compared to controls. Our results suggest a role for incretins in AIS development and severity.
format	article
author	Emilie Normand Anita Franco Alain Moreau Valérie Marcil
author_facet	Emilie Normand Anita Franco Alain Moreau Valérie Marcil
author_sort	Emilie Normand
title	Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts
title_short	Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts
title_full	Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts
title_fullStr	Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts
title_full_unstemmed	Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts
title_sort	dipeptidyl peptidase-4 and adolescent idiopathic scoliosis: expression in osteoblasts
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/39ecfbac05f2488ba6eb6d1dbf57f2f4
work_keys_str_mv	AT emilienormand dipeptidylpeptidase4andadolescentidiopathicscoliosisexpressioninosteoblasts AT anitafranco dipeptidylpeptidase4andadolescentidiopathicscoliosisexpressioninosteoblasts AT alainmoreau dipeptidylpeptidase4andadolescentidiopathicscoliosisexpressioninosteoblasts AT valeriemarcil dipeptidylpeptidase4andadolescentidiopathicscoliosisexpressioninosteoblasts
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Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts

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