The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes

Abstract LPS-ligation to CD14/TLR-4 on monocytes/macrophages triggers the production of IL-12-family cytokines. IL12/18 promote TH1-differentiation, counteracting the TH2-driven asthma. Therefore, CD14 modulation could alter the TH2-differentiation and should be taken into account when studying asth...

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Autores principales:	J. J. Nieto-Fontarigo, F. J. Salgado, M. E. San-José, M. J. Cruz, A. Casas-Fernández, M. J. Gómez-Conde, L. Valdés-Cuadrado, M. Á. García-González, P. Arias, M. Nogueira, F. J. González-Barcala
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39f36710d7c84494bb6210b4d752f648
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spelling	oai:doaj.org-article:39f36710d7c84494bb6210b4d752f6482021-12-02T15:08:40ZThe CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes10.1038/s41598-018-20483-12045-2322https://doaj.org/article/39f36710d7c84494bb6210b4d752f6482018-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20483-1https://doaj.org/toc/2045-2322Abstract LPS-ligation to CD14/TLR-4 on monocytes/macrophages triggers the production of IL-12-family cytokines. IL12/18 promote TH1-differentiation, counteracting the TH2-driven asthma. Therefore, CD14 modulation could alter the TH2-differentiation and should be taken into account when studying asthma. To analyse the alteration in CD14 levels and its association with CD14 (−159 C/T) SNP (rs2569190) in Caucasian adults with stable allergic asthma, we performed a cross-sectional study (277 healthy subjects vs. 277 patients) where clinical parameters, CD14 values and the CD14 (−159 C/T) SNP were studied. Apart from typical biomarkers, we found an increment of neuron-specific enolase (NSE) in allergic asthma, probably linked to monocyte activity. Indeed, we evidenced increased monocyte numbers, but lower CD14 expression and normalised sCD14 values in patients. Moreover, we noticed an association of the T allele (P = 0.0162) and TT genotype (P = 0.0196) of the CD14 SNP with a decreased risk of allergic asthma and augmented sCD14 levels. In conclusion, monocyte CD14 expression and normalized sCD14 values were reduced in stable state asthmatics, and this could be related to the presence of an expanded CD14low monocyte subset. This study also demonstrates that the CD14 (−159 C/T) polymorphism is a risk factor for moderate-severe allergic asthma in adult Caucasians.J. J. Nieto-FontarigoF. J. SalgadoM. E. San-JoséM. J. CruzA. Casas-FernándezM. J. Gómez-CondeL. Valdés-CuadradoM. Á. García-GonzálezP. AriasM. NogueiraF. J. González-BarcalaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. J. Nieto-Fontarigo F. J. Salgado M. E. San-José M. J. Cruz A. Casas-Fernández M. J. Gómez-Conde L. Valdés-Cuadrado M. Á. García-González P. Arias M. Nogueira F. J. González-Barcala The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
description	Abstract LPS-ligation to CD14/TLR-4 on monocytes/macrophages triggers the production of IL-12-family cytokines. IL12/18 promote TH1-differentiation, counteracting the TH2-driven asthma. Therefore, CD14 modulation could alter the TH2-differentiation and should be taken into account when studying asthma. To analyse the alteration in CD14 levels and its association with CD14 (−159 C/T) SNP (rs2569190) in Caucasian adults with stable allergic asthma, we performed a cross-sectional study (277 healthy subjects vs. 277 patients) where clinical parameters, CD14 values and the CD14 (−159 C/T) SNP were studied. Apart from typical biomarkers, we found an increment of neuron-specific enolase (NSE) in allergic asthma, probably linked to monocyte activity. Indeed, we evidenced increased monocyte numbers, but lower CD14 expression and normalised sCD14 values in patients. Moreover, we noticed an association of the T allele (P = 0.0162) and TT genotype (P = 0.0196) of the CD14 SNP with a decreased risk of allergic asthma and augmented sCD14 levels. In conclusion, monocyte CD14 expression and normalized sCD14 values were reduced in stable state asthmatics, and this could be related to the presence of an expanded CD14low monocyte subset. This study also demonstrates that the CD14 (−159 C/T) polymorphism is a risk factor for moderate-severe allergic asthma in adult Caucasians.
format	article
author	J. J. Nieto-Fontarigo F. J. Salgado M. E. San-José M. J. Cruz A. Casas-Fernández M. J. Gómez-Conde L. Valdés-Cuadrado M. Á. García-González P. Arias M. Nogueira F. J. González-Barcala
author_facet	J. J. Nieto-Fontarigo F. J. Salgado M. E. San-José M. J. Cruz A. Casas-Fernández M. J. Gómez-Conde L. Valdés-Cuadrado M. Á. García-González P. Arias M. Nogueira F. J. González-Barcala
author_sort	J. J. Nieto-Fontarigo
title	The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
title_short	The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
title_full	The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
title_fullStr	The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
title_full_unstemmed	The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes
title_sort	cd14 (−159 c/t) snp is associated with scd14 levels and allergic asthma, but not with cd14 expression on monocytes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/39f36710d7c84494bb6210b4d752f648
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The CD14 (−159 C/T) SNP is associated with sCD14 levels and allergic asthma, but not with CD14 expression on monocytes

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