Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan

Colistin is the last resort antimicrobial for treating multidrug-resistant gram-negative bacterial infections. The plasmid-mediated colistin resistance gene, mcr-1, crucially influences colistin’s resistance transmission. Human fecal carriages of mcr-1-positive Escherichia coli (E. coli) were detect...

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Autores principales:	Pin-Chieh Wu, Ming-Fang Cheng, Wan-Ling Chen, Wan-Yu Hung, Jiun-Ling Wang, Chih-Hsin Hung
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	mcr-1 risk factor prevalence community children fecal carriage Taiwan Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/39fab63bfddb41c8969d9720d6b46c76
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spelling	oai:doaj.org-article:39fab63bfddb41c8969d9720d6b46c762021-11-19T06:43:33ZRisk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan1664-302X10.3389/fmicb.2021.748525https://doaj.org/article/39fab63bfddb41c8969d9720d6b46c762021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.748525/fullhttps://doaj.org/toc/1664-302XColistin is the last resort antimicrobial for treating multidrug-resistant gram-negative bacterial infections. The plasmid-mediated colistin resistance gene, mcr-1, crucially influences colistin’s resistance transmission. Human fecal carriages of mcr-1-positive Escherichia coli (E. coli) were detected in many regions worldwide; however, only a few studies have focused on children. Therefore, we identified the prevalence and risk factors of mcr-1-positive E. coli in fecal carriages among community children in Southern Taiwan. In this study, 510 stool samples were collected from April 2016 to August 2019 from the pediatric department at a medical center in Southern Taiwan. These samples were collected within 3 days after admission and were all screened for the presence of the mcr-1 gene. Diet habits, travel history, pet contact, and medical history were also obtained from participants to analyze the risk factors of their fecal carriages to mcr-1-positive E. coli. Antimicrobial susceptibility testing was determined using the VITEK 2 system and the broth microdilution test. Twelve mcr-1-positive E. coli. were isolated from 2.4% of the fecal samples. Through multivariate analysis, frequent chicken consumption (at least 3 times per week) had a significantly positive association with the presence of mcr-1-positive E. coli in fecal carriages (adjust odds ratio 6.60, 95% confidence interval1.58– 27.62, p = 0.033). Additionally, multidrug resistance was more common in mcr-1-positive E. coli. (75.0% vs. 39.5%, p = 0.031) than in non-mcr-1-positive Escherichia coli. Furthermore, the percentage of extraintestinal pathogenic E. coli in mcr-1-positive isolates was 83.3%. Some multi-locus sequence types in our mcr-1-positive E. coli were also similar to those isolated from food animals in the literature. The prevalence of fecal carriages of mcr-1-positive E. coli was low among community children in Southern Taiwan. Our data shows that chicken consumption with a higher frequency increases the risk of mcr-1-positive E. coli. in fecal carriages.Pin-Chieh WuPin-Chieh WuPin-Chieh WuMing-Fang ChengMing-Fang ChengMing-Fang ChengMing-Fang ChengWan-Ling ChenWan-Ling ChenWan-Yu HungJiun-Ling WangJiun-Ling WangChih-Hsin HungFrontiers Media S.A.articlemcr-1risk factorprevalencecommunity childrenfecal carriageTaiwanMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mcr-1 risk factor prevalence community children fecal carriage Taiwan Microbiology QR1-502
spellingShingle	mcr-1 risk factor prevalence community children fecal carriage Taiwan Microbiology QR1-502 Pin-Chieh Wu Pin-Chieh Wu Pin-Chieh Wu Ming-Fang Cheng Ming-Fang Cheng Ming-Fang Cheng Ming-Fang Cheng Wan-Ling Chen Wan-Ling Chen Wan-Yu Hung Jiun-Ling Wang Jiun-Ling Wang Chih-Hsin Hung Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan
description	Colistin is the last resort antimicrobial for treating multidrug-resistant gram-negative bacterial infections. The plasmid-mediated colistin resistance gene, mcr-1, crucially influences colistin’s resistance transmission. Human fecal carriages of mcr-1-positive Escherichia coli (E. coli) were detected in many regions worldwide; however, only a few studies have focused on children. Therefore, we identified the prevalence and risk factors of mcr-1-positive E. coli in fecal carriages among community children in Southern Taiwan. In this study, 510 stool samples were collected from April 2016 to August 2019 from the pediatric department at a medical center in Southern Taiwan. These samples were collected within 3 days after admission and were all screened for the presence of the mcr-1 gene. Diet habits, travel history, pet contact, and medical history were also obtained from participants to analyze the risk factors of their fecal carriages to mcr-1-positive E. coli. Antimicrobial susceptibility testing was determined using the VITEK 2 system and the broth microdilution test. Twelve mcr-1-positive E. coli. were isolated from 2.4% of the fecal samples. Through multivariate analysis, frequent chicken consumption (at least 3 times per week) had a significantly positive association with the presence of mcr-1-positive E. coli in fecal carriages (adjust odds ratio 6.60, 95% confidence interval1.58– 27.62, p = 0.033). Additionally, multidrug resistance was more common in mcr-1-positive E. coli. (75.0% vs. 39.5%, p = 0.031) than in non-mcr-1-positive Escherichia coli. Furthermore, the percentage of extraintestinal pathogenic E. coli in mcr-1-positive isolates was 83.3%. Some multi-locus sequence types in our mcr-1-positive E. coli were also similar to those isolated from food animals in the literature. The prevalence of fecal carriages of mcr-1-positive E. coli was low among community children in Southern Taiwan. Our data shows that chicken consumption with a higher frequency increases the risk of mcr-1-positive E. coli. in fecal carriages.
format	article
author	Pin-Chieh Wu Pin-Chieh Wu Pin-Chieh Wu Ming-Fang Cheng Ming-Fang Cheng Ming-Fang Cheng Ming-Fang Cheng Wan-Ling Chen Wan-Ling Chen Wan-Yu Hung Jiun-Ling Wang Jiun-Ling Wang Chih-Hsin Hung
author_facet	Pin-Chieh Wu Pin-Chieh Wu Pin-Chieh Wu Ming-Fang Cheng Ming-Fang Cheng Ming-Fang Cheng Ming-Fang Cheng Wan-Ling Chen Wan-Ling Chen Wan-Yu Hung Jiun-Ling Wang Jiun-Ling Wang Chih-Hsin Hung
author_sort	Pin-Chieh Wu
title	Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan
title_short	Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan
title_full	Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan
title_fullStr	Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan
title_full_unstemmed	Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan
title_sort	risk factors and prevalence of mcr-1-positive escherichia coli in fecal carriages among community children in southern taiwan
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/39fab63bfddb41c8969d9720d6b46c76
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Risk Factors and Prevalence of mcr-1-Positive Escherichia coli in Fecal Carriages Among Community Children in Southern Taiwan

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