Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant

In the present study, effect of size and age of hypocotyl explant on in vitro organogenetic responses was assessed in eggplant cv. Manjarigota. Size and age did not affect callus-initiation response, but showed marked influence on shoot regeneration response. Hypocotyl explants 1.5cm long showed hig...

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Autores principales:	D P Prakash, B S Deepali, Y L Ramachandra, Lalitha Anand, Vageeshbabu S Hanur
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2012
Materias:	eggplant shoot regeneration age and size hypocotyl explants Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/39fe5c4825984903941e8f03080f7ea0
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spelling	oai:doaj.org-article:39fe5c4825984903941e8f03080f7ea02021-12-02T08:01:04ZEffect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant0973-354X2582-4899https://doaj.org/article/39fe5c4825984903941e8f03080f7ea02012-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/376https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899In the present study, effect of size and age of hypocotyl explant on in vitro organogenetic responses was assessed in eggplant cv. Manjarigota. Size and age did not affect callus-initiation response, but showed marked influence on shoot regeneration response. Hypocotyl explants 1.5cm long showed highest shoot regeneration response (77.4%); either increase or decrease in size resulted in reduced response. Five to 15 day old hypocotyl explants showed direct shoot regeneration from cut ends, whereas 20-30 day old hypocotyl explants showed indirect shoot regeneration from callus produced on cut ends. Five day old explants were most responsive, with highest (91.23%) and thirty day old explants least responsive with reference to shoot regeneration response (20.85%). Shoot regeneration frequency decreased with increasing age, whereas shoot regeneration efficiency increased with increasing age of hypocotyl explants.D P PrakashB S DeepaliY L RamachandraLalitha AnandVageeshbabu S HanurSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleeggplantshoot regenerationage and sizehypocotyl explantsPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 7, Iss 2, Pp 203-205 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	eggplant shoot regeneration age and size hypocotyl explants Plant culture SB1-1110
spellingShingle	eggplant shoot regeneration age and size hypocotyl explants Plant culture SB1-1110 D P Prakash B S Deepali Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
description	In the present study, effect of size and age of hypocotyl explant on in vitro organogenetic responses was assessed in eggplant cv. Manjarigota. Size and age did not affect callus-initiation response, but showed marked influence on shoot regeneration response. Hypocotyl explants 1.5cm long showed highest shoot regeneration response (77.4%); either increase or decrease in size resulted in reduced response. Five to 15 day old hypocotyl explants showed direct shoot regeneration from cut ends, whereas 20-30 day old hypocotyl explants showed indirect shoot regeneration from callus produced on cut ends. Five day old explants were most responsive, with highest (91.23%) and thirty day old explants least responsive with reference to shoot regeneration response (20.85%). Shoot regeneration frequency decreased with increasing age, whereas shoot regeneration efficiency increased with increasing age of hypocotyl explants.
format	article
author	D P Prakash B S Deepali Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur
author_facet	D P Prakash B S Deepali Y L Ramachandra Lalitha Anand Vageeshbabu S Hanur
author_sort	D P Prakash
title	Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
title_short	Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
title_full	Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
title_fullStr	Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
title_full_unstemmed	Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
title_sort	effect of age and size of hypocotyl explant on in vitro shoot regeneration in eggplant
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2012
url	https://doaj.org/article/39fe5c4825984903941e8f03080f7ea0
work_keys_str_mv	AT dpprakash effectofageandsizeofhypocotylexplantoninvitroshootregenerationineggplant AT bsdeepali effectofageandsizeofhypocotylexplantoninvitroshootregenerationineggplant AT ylramachandra effectofageandsizeofhypocotylexplantoninvitroshootregenerationineggplant AT lalithaanand effectofageandsizeofhypocotylexplantoninvitroshootregenerationineggplant AT vageeshbabushanur effectofageandsizeofhypocotylexplantoninvitroshootregenerationineggplant
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Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant

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