Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.

<h4>Background</h4>Periventricular white matter injury (PWMI) is a common form of brain injury sustained by preterm infants. A major factor that predisposes to PWMI is hypoxia. Because oligodendrocytes (OLs) are responsible for myelination of axons, abnormal OL development or function ma...

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Autores principales:	Ravi Shankar Akundi, Scott A Rivkees
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a03ac29900f407b81fd6888b1000149
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spelling	oai:doaj.org-article:3a03ac29900f407b81fd6888b10001492021-11-25T06:16:52ZHypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.1932-620310.1371/journal.pone.0004739https://doaj.org/article/3a03ac29900f407b81fd6888b10001492009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19270736/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Periventricular white matter injury (PWMI) is a common form of brain injury sustained by preterm infants. A major factor that predisposes to PWMI is hypoxia. Because oligodendrocytes (OLs) are responsible for myelination of axons, abnormal OL development or function may affect brain myelination. At present our understanding of the influences of hypoxia on OL development is limited. To examine isolated effects of hypoxia on OLs, we examined the influences of hypoxia on OL development in vitro.<h4>Methodology/findings</h4>Cultures of oligodendrocyte precursor cells (OPCs) were prepared from mixed glial cultures and were 99% pure. OPCs were maintained at 21% O(2) or hypoxia (1% or 4% O(2)) for up to 7 days. We observed that 1% O(2) lead to an increase in the proportion of myelin basic protein (MBP)-positive OLs after 1 week in culture, and a decrease in the proportion of platelet-derived growth factor receptor alpha (PDGFRalpha)-positive cells suggesting premature OL maturation. Increased expression of the cell cycle regulatory proteins p27(Kip1) and phospho-cdc2, which play a role in OL differentiation, was seen as well.<h4>Conclusions</h4>These results show that hypoxia interferes with the normal process of OL differentiation by inducing premature OPC maturation.Ravi Shankar AkundiScott A RivkeesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4739 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ravi Shankar Akundi Scott A Rivkees Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.
description	<h4>Background</h4>Periventricular white matter injury (PWMI) is a common form of brain injury sustained by preterm infants. A major factor that predisposes to PWMI is hypoxia. Because oligodendrocytes (OLs) are responsible for myelination of axons, abnormal OL development or function may affect brain myelination. At present our understanding of the influences of hypoxia on OL development is limited. To examine isolated effects of hypoxia on OLs, we examined the influences of hypoxia on OL development in vitro.<h4>Methodology/findings</h4>Cultures of oligodendrocyte precursor cells (OPCs) were prepared from mixed glial cultures and were 99% pure. OPCs were maintained at 21% O(2) or hypoxia (1% or 4% O(2)) for up to 7 days. We observed that 1% O(2) lead to an increase in the proportion of myelin basic protein (MBP)-positive OLs after 1 week in culture, and a decrease in the proportion of platelet-derived growth factor receptor alpha (PDGFRalpha)-positive cells suggesting premature OL maturation. Increased expression of the cell cycle regulatory proteins p27(Kip1) and phospho-cdc2, which play a role in OL differentiation, was seen as well.<h4>Conclusions</h4>These results show that hypoxia interferes with the normal process of OL differentiation by inducing premature OPC maturation.
format	article
author	Ravi Shankar Akundi Scott A Rivkees
author_facet	Ravi Shankar Akundi Scott A Rivkees
author_sort	Ravi Shankar Akundi
title	Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.
title_short	Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.
title_full	Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.
title_fullStr	Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.
title_full_unstemmed	Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.
title_sort	hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/3a03ac29900f407b81fd6888b1000149
work_keys_str_mv	AT ravishankarakundi hypoxiaalterscellcycleregulatoryproteinexpressionandinducesprematurematurationofoligodendrocyteprecursorcells AT scottarivkees hypoxiaalterscellcycleregulatoryproteinexpressionandinducesprematurematurationofoligodendrocyteprecursorcells
_version_	1718413976015994880

Hypoxia alters cell cycle regulatory protein expression and induces premature maturation of oligodendrocyte precursor cells.

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