Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing
Abstract A key predictor of morbidity and mortality for patients with a bloodstream infection is time to appropriate antimicrobial therapy. Accelerating antimicrobial susceptibility testing from positive blood cultures is therefore key to improving patient outcomes, yet traditional laboratory approa...
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Nature Portfolio
2021
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oai:doaj.org-article:3a0d158affe14e59898388fa93ad2ee12021-12-02T17:51:21ZNovel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing10.1038/s41598-021-90830-22045-2322https://doaj.org/article/3a0d158affe14e59898388fa93ad2ee12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90830-2https://doaj.org/toc/2045-2322Abstract A key predictor of morbidity and mortality for patients with a bloodstream infection is time to appropriate antimicrobial therapy. Accelerating antimicrobial susceptibility testing from positive blood cultures is therefore key to improving patient outcomes, yet traditional laboratory approaches can require 2–4 days for actionable results. The eQUANT—a novel instrument utilizing electrical biosensors—produces a standardized inoculum equivalent to a 0.5 McFarland directly from positive blood cultures. This proof-of-concept study demonstrates that eQUANT inocula prepared from clinically significant species of Enterobacterales were comparable to 0.5 McF inocula generated from bacterial colonies in both CFU/ml concentration and performance in antimicrobial susceptibility testing, with ≥ 95% essential and categorical agreement for VITEK2 and disk diffusion. The eQUANT, combined with a rapid, direct from positive blood culture identification technique, can allow the clinical laboratory to begin antimicrobial susceptibility testing using a standardized inoculum approximately 2–3 h after a blood culture flags positive. This has the potential to improve clinical practice by accelerating conventional antimicrobial susceptibility testing and the resulting targeted antibiotic therapy.Suzanne PutneyAndrew H. TheissNitin K. RajanEszter DeakCreighton BuieYvonne NgoHima ShahVictoria YuanElizabeth Botbol-PonteAdrian Hoyos-UriasOren KnopfmacherCatherine A. HoganNiaz BanaeiMeike S. HergetNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| institution |
DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Suzanne Putney Andrew H. Theiss Nitin K. Rajan Eszter Deak Creighton Buie Yvonne Ngo Hima Shah Victoria Yuan Elizabeth Botbol-Ponte Adrian Hoyos-Urias Oren Knopfmacher Catherine A. Hogan Niaz Banaei Meike S. Herget Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing |
| description |
Abstract A key predictor of morbidity and mortality for patients with a bloodstream infection is time to appropriate antimicrobial therapy. Accelerating antimicrobial susceptibility testing from positive blood cultures is therefore key to improving patient outcomes, yet traditional laboratory approaches can require 2–4 days for actionable results. The eQUANT—a novel instrument utilizing electrical biosensors—produces a standardized inoculum equivalent to a 0.5 McFarland directly from positive blood cultures. This proof-of-concept study demonstrates that eQUANT inocula prepared from clinically significant species of Enterobacterales were comparable to 0.5 McF inocula generated from bacterial colonies in both CFU/ml concentration and performance in antimicrobial susceptibility testing, with ≥ 95% essential and categorical agreement for VITEK2 and disk diffusion. The eQUANT, combined with a rapid, direct from positive blood culture identification technique, can allow the clinical laboratory to begin antimicrobial susceptibility testing using a standardized inoculum approximately 2–3 h after a blood culture flags positive. This has the potential to improve clinical practice by accelerating conventional antimicrobial susceptibility testing and the resulting targeted antibiotic therapy. |
| format |
article |
| author |
Suzanne Putney Andrew H. Theiss Nitin K. Rajan Eszter Deak Creighton Buie Yvonne Ngo Hima Shah Victoria Yuan Elizabeth Botbol-Ponte Adrian Hoyos-Urias Oren Knopfmacher Catherine A. Hogan Niaz Banaei Meike S. Herget |
| author_facet |
Suzanne Putney Andrew H. Theiss Nitin K. Rajan Eszter Deak Creighton Buie Yvonne Ngo Hima Shah Victoria Yuan Elizabeth Botbol-Ponte Adrian Hoyos-Urias Oren Knopfmacher Catherine A. Hogan Niaz Banaei Meike S. Herget |
| author_sort |
Suzanne Putney |
| title |
Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing |
| title_short |
Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing |
| title_full |
Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing |
| title_fullStr |
Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing |
| title_full_unstemmed |
Novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing |
| title_sort |
novel electronic biosensor for automated inoculum preparation to accelerate antimicrobial susceptibility testing |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/3a0d158affe14e59898388fa93ad2ee1 |
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