Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids

Abstract In bovine species, mitochondrial DNA polymorphisms and their correlation to productive or reproductive performances have been widely reported across breeds and individuals. However, experimental evidence of this correlation has never been provided. In order to identify differences among bov...

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Autores principales:	Jikun Wang, Hai Xiang, Langqing Liu, Minghua Kong, Tao Yin, Xingbo Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a0f475c6146482187fc6aa0304726a6
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spelling	oai:doaj.org-article:3a0f475c6146482187fc6aa0304726a62021-12-02T16:06:39ZMitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids10.1038/s41598-017-04457-32045-2322https://doaj.org/article/3a0f475c6146482187fc6aa0304726a62017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04457-3https://doaj.org/toc/2045-2322Abstract In bovine species, mitochondrial DNA polymorphisms and their correlation to productive or reproductive performances have been widely reported across breeds and individuals. However, experimental evidence of this correlation has never been provided. In order to identify differences among bovine mtDNA haplotypes, transmitochondrial cybrids were generated, with the nucleus from MAC-T cell line, derived from a Holstein dairy cow (Bos taurus) and mitochondria from either primary cell line derived from a domestic Chinese native beef Luxi cattle breed or central Asian domestic yak (Bos grunniens). Yak primary cells illustrated a stronger metabolic capacity than that of Luxi. However, all yak cybrid parameters illustrated a drop in relative yak mtDNA compared to Luxi mtDNA, in line with a mitonuclear imbalance in yak interspecies cybrid. Luxi has 250 divergent variations relative to the mitogenome of Holsteins. In cybrids there were generally higher rates of oxygen consumption (OCR) and extracellular acidification (ECAR), and lower mRNA expression levels of nuclear-encoded mitochondrial genes, potentially reflecting active energy metabolism and cellular stress resistance. The results demonstrate that functional differences exist between bovine cybrid cells. While cybrid viability was similar between Holstein and Luxi breeds, the mitonuclear mismatch caused a marked metabolic dysfunction in cattle:yak cybrid species.Jikun WangHai XiangLangqing LiuMinghua KongTao YinXingbo ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jikun Wang Hai Xiang Langqing Liu Minghua Kong Tao Yin Xingbo Zhao Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
description	Abstract In bovine species, mitochondrial DNA polymorphisms and their correlation to productive or reproductive performances have been widely reported across breeds and individuals. However, experimental evidence of this correlation has never been provided. In order to identify differences among bovine mtDNA haplotypes, transmitochondrial cybrids were generated, with the nucleus from MAC-T cell line, derived from a Holstein dairy cow (Bos taurus) and mitochondria from either primary cell line derived from a domestic Chinese native beef Luxi cattle breed or central Asian domestic yak (Bos grunniens). Yak primary cells illustrated a stronger metabolic capacity than that of Luxi. However, all yak cybrid parameters illustrated a drop in relative yak mtDNA compared to Luxi mtDNA, in line with a mitonuclear imbalance in yak interspecies cybrid. Luxi has 250 divergent variations relative to the mitogenome of Holsteins. In cybrids there were generally higher rates of oxygen consumption (OCR) and extracellular acidification (ECAR), and lower mRNA expression levels of nuclear-encoded mitochondrial genes, potentially reflecting active energy metabolism and cellular stress resistance. The results demonstrate that functional differences exist between bovine cybrid cells. While cybrid viability was similar between Holstein and Luxi breeds, the mitonuclear mismatch caused a marked metabolic dysfunction in cattle:yak cybrid species.
format	article
author	Jikun Wang Hai Xiang Langqing Liu Minghua Kong Tao Yin Xingbo Zhao
author_facet	Jikun Wang Hai Xiang Langqing Liu Minghua Kong Tao Yin Xingbo Zhao
author_sort	Jikun Wang
title	Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
title_short	Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
title_full	Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
title_fullStr	Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
title_full_unstemmed	Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
title_sort	mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3a0f475c6146482187fc6aa0304726a6
work_keys_str_mv	AT jikunwang mitochondrialhaplotypesinfluencemetabolictraitsacrossbovineinterandintraspeciescybrids AT haixiang mitochondrialhaplotypesinfluencemetabolictraitsacrossbovineinterandintraspeciescybrids AT langqingliu mitochondrialhaplotypesinfluencemetabolictraitsacrossbovineinterandintraspeciescybrids AT minghuakong mitochondrialhaplotypesinfluencemetabolictraitsacrossbovineinterandintraspeciescybrids AT taoyin mitochondrialhaplotypesinfluencemetabolictraitsacrossbovineinterandintraspeciescybrids AT xingbozhao mitochondrialhaplotypesinfluencemetabolictraitsacrossbovineinterandintraspeciescybrids
_version_	1718384897138098176

Mitochondrial haplotypes influence metabolic traits across bovine inter- and intra-species cybrids

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