Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease

Abstract Calciprotein particles (CPP) are solid-phase calcium-phosphate bound to serum protein fetuin-A and dispersed as colloids in the blood. Recent clinical studies indicated that serum CPP levels were increased with decline of renal function and associated with inflammation and vascular calcific...

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Autores principales:	Yutaka Miura, Yoshitaka Iwazu, Kazuhiro Shiizaki, Tetsu Akimoto, Kazuhiko Kotani, Masahiko Kurabayashi, Hiroshi Kurosu, Makoto Kuro-o
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a2096288f364f7893e361803aa224a3
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spelling	oai:doaj.org-article:3a2096288f364f7893e361803aa224a32021-12-02T15:08:11ZIdentification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease10.1038/s41598-018-19677-42045-2322https://doaj.org/article/3a2096288f364f7893e361803aa224a32018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19677-4https://doaj.org/toc/2045-2322Abstract Calciprotein particles (CPP) are solid-phase calcium-phosphate bound to serum protein fetuin-A and dispersed as colloids in the blood. Recent clinical studies indicated that serum CPP levels were increased with decline of renal function and associated with inflammation and vascular calcification. However, CPP assays used in these studies measured only a part of CPP over a certain particle size and density. Here we show that such CPP are mostly artifacts generated during processing of serum samples in vitro. The native CPP in fresh plasma are smaller in size and lower in density than those artifactual CPP, composed of fetuin-A carrying amorphous and/or crystalline calcium-phosphate, and increased primarily with serum phosphate levels. We have identified several physicochemical factors that promote aggregation/dissolution of CPP and transition of the calcium-phosphate from the amorphous phase to the crystalline phase in vitro, including addition of anti-coagulants, composition of buffer for sample dilution, the number of freeze-thaw cycles, the speed for sample freezing, and how many hours the samples were left at what temperature. Therefore, it is of critical importance to standardize these factors during sample preparation in clinical studies on CPP and to investigate the biological activity of the native CPP.Yutaka MiuraYoshitaka IwazuKazuhiro ShiizakiTetsu AkimotoKazuhiko KotaniMasahiko KurabayashiHiroshi KurosuMakoto Kuro-oNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yutaka Miura Yoshitaka Iwazu Kazuhiro Shiizaki Tetsu Akimoto Kazuhiko Kotani Masahiko Kurabayashi Hiroshi Kurosu Makoto Kuro-o Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease
description	Abstract Calciprotein particles (CPP) are solid-phase calcium-phosphate bound to serum protein fetuin-A and dispersed as colloids in the blood. Recent clinical studies indicated that serum CPP levels were increased with decline of renal function and associated with inflammation and vascular calcification. However, CPP assays used in these studies measured only a part of CPP over a certain particle size and density. Here we show that such CPP are mostly artifacts generated during processing of serum samples in vitro. The native CPP in fresh plasma are smaller in size and lower in density than those artifactual CPP, composed of fetuin-A carrying amorphous and/or crystalline calcium-phosphate, and increased primarily with serum phosphate levels. We have identified several physicochemical factors that promote aggregation/dissolution of CPP and transition of the calcium-phosphate from the amorphous phase to the crystalline phase in vitro, including addition of anti-coagulants, composition of buffer for sample dilution, the number of freeze-thaw cycles, the speed for sample freezing, and how many hours the samples were left at what temperature. Therefore, it is of critical importance to standardize these factors during sample preparation in clinical studies on CPP and to investigate the biological activity of the native CPP.
format	article
author	Yutaka Miura Yoshitaka Iwazu Kazuhiro Shiizaki Tetsu Akimoto Kazuhiko Kotani Masahiko Kurabayashi Hiroshi Kurosu Makoto Kuro-o
author_facet	Yutaka Miura Yoshitaka Iwazu Kazuhiro Shiizaki Tetsu Akimoto Kazuhiko Kotani Masahiko Kurabayashi Hiroshi Kurosu Makoto Kuro-o
author_sort	Yutaka Miura
title	Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease
title_short	Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease
title_full	Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease
title_fullStr	Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease
title_full_unstemmed	Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease
title_sort	identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3a2096288f364f7893e361803aa224a3
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Identification and quantification of plasma calciprotein particles with distinct physical properties in patients with chronic kidney disease

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