Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection

Abstract Salmonella Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important...

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Autores principales:	Heather E. Hulme, Lynsey M. Meikle, Hannah Wessel, Nicole Strittmatter, John Swales, Carolyn Thomson, Anna Nilsson, Robert J. B. Nibbs, Simon Milling, Per E. Andren, C. Logan Mackay, Alex Dexter, Josephine Bunch, Richard J. A. Goodwin, Richard Burchmore, Daniel M. Wall
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a24c8a8c4bd40bd88a9c66a0de21e92
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spelling	oai:doaj.org-article:3a24c8a8c4bd40bd88a9c66a0de21e922021-12-02T15:06:17ZMass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection10.1038/s41598-017-03100-52045-2322https://doaj.org/article/3a24c8a8c4bd40bd88a9c66a0de21e922017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03100-5https://doaj.org/toc/2045-2322Abstract Salmonella Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during S. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of S. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of S. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169+ sub capsular sinus macrophages was disrupted. MLN cells cultured ex vivo and treated with PalC had reduced CD4+CD25+ T cells and an increased number of B220+CD19+ B cells. The reduction in CD4+CD25+ T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome.Heather E. HulmeLynsey M. MeikleHannah WesselNicole StrittmatterJohn SwalesCarolyn ThomsonAnna NilssonRobert J. B. NibbsSimon MillingPer E. AndrenC. Logan MackayAlex DexterJosephine BunchRichard J. A. GoodwinRichard BurchmoreDaniel M. WallNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heather E. Hulme Lynsey M. Meikle Hannah Wessel Nicole Strittmatter John Swales Carolyn Thomson Anna Nilsson Robert J. B. Nibbs Simon Milling Per E. Andren C. Logan Mackay Alex Dexter Josephine Bunch Richard J. A. Goodwin Richard Burchmore Daniel M. Wall Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
description	Abstract Salmonella Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during S. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of S. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of S. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169+ sub capsular sinus macrophages was disrupted. MLN cells cultured ex vivo and treated with PalC had reduced CD4+CD25+ T cells and an increased number of B220+CD19+ B cells. The reduction in CD4+CD25+ T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome.
format	article
author	Heather E. Hulme Lynsey M. Meikle Hannah Wessel Nicole Strittmatter John Swales Carolyn Thomson Anna Nilsson Robert J. B. Nibbs Simon Milling Per E. Andren C. Logan Mackay Alex Dexter Josephine Bunch Richard J. A. Goodwin Richard Burchmore Daniel M. Wall
author_facet	Heather E. Hulme Lynsey M. Meikle Hannah Wessel Nicole Strittmatter John Swales Carolyn Thomson Anna Nilsson Robert J. B. Nibbs Simon Milling Per E. Andren C. Logan Mackay Alex Dexter Josephine Bunch Richard J. A. Goodwin Richard Burchmore Daniel M. Wall
author_sort	Heather E. Hulme
title	Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
title_short	Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
title_full	Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
title_fullStr	Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
title_full_unstemmed	Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
title_sort	mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during salmonella typhimurium infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3a24c8a8c4bd40bd88a9c66a0de21e92
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Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection

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