The V protein of canine distemper virus is required for virus replication in human epithelial cells.

Canine distemper virus (CDV) becomes able to use human receptors through a single amino acid substitution in the H protein. In addition, CDV strains possessing an intact C protein replicate well in human epithelial H358 cells. The present study showed that CDV strain 007Lm, which was isolated from l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Noriyuki Otsuki, Yuichiro Nakatsu, Toru Kubota, Tsuyoshi Sekizuka, Fumio Seki, Kouji Sakai, Makoto Kuroda, Ryoji Yamaguchi, Makoto Takeda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a25febd344543228c84950a70bfc556
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3a25febd344543228c84950a70bfc556
record_format	dspace
spelling	oai:doaj.org-article:3a25febd344543228c84950a70bfc5562021-11-18T08:41:39ZThe V protein of canine distemper virus is required for virus replication in human epithelial cells.1932-620310.1371/journal.pone.0082343https://doaj.org/article/3a25febd344543228c84950a70bfc5562013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24358174/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Canine distemper virus (CDV) becomes able to use human receptors through a single amino acid substitution in the H protein. In addition, CDV strains possessing an intact C protein replicate well in human epithelial H358 cells. The present study showed that CDV strain 007Lm, which was isolated from lymph node tissue of a dog with distemper, failed to replicate in H358 cells, although it possessed an intact C protein. Sequence analyses suggested that a cysteine-to-tyrosine substitution at position 267 of the V protein caused this growth defect. Analyses using H358 cells constitutively expressing the CDV V protein showed that the V protein with a cysteine, but not that with a tyrosine, at this position effectively blocked the interferon-stimulated signal transduction pathway, and supported virus replication of 007Lm in H358 cells. Thus, the V protein as well as the C protein appears to be functional and essential for CDV replication in human epithelial cells.Noriyuki OtsukiYuichiro NakatsuToru KubotaTsuyoshi SekizukaFumio SekiKouji SakaiMakoto KurodaRyoji YamaguchiMakoto TakedaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82343 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Noriyuki Otsuki Yuichiro Nakatsu Toru Kubota Tsuyoshi Sekizuka Fumio Seki Kouji Sakai Makoto Kuroda Ryoji Yamaguchi Makoto Takeda The V protein of canine distemper virus is required for virus replication in human epithelial cells.
description	Canine distemper virus (CDV) becomes able to use human receptors through a single amino acid substitution in the H protein. In addition, CDV strains possessing an intact C protein replicate well in human epithelial H358 cells. The present study showed that CDV strain 007Lm, which was isolated from lymph node tissue of a dog with distemper, failed to replicate in H358 cells, although it possessed an intact C protein. Sequence analyses suggested that a cysteine-to-tyrosine substitution at position 267 of the V protein caused this growth defect. Analyses using H358 cells constitutively expressing the CDV V protein showed that the V protein with a cysteine, but not that with a tyrosine, at this position effectively blocked the interferon-stimulated signal transduction pathway, and supported virus replication of 007Lm in H358 cells. Thus, the V protein as well as the C protein appears to be functional and essential for CDV replication in human epithelial cells.
format	article
author	Noriyuki Otsuki Yuichiro Nakatsu Toru Kubota Tsuyoshi Sekizuka Fumio Seki Kouji Sakai Makoto Kuroda Ryoji Yamaguchi Makoto Takeda
author_facet	Noriyuki Otsuki Yuichiro Nakatsu Toru Kubota Tsuyoshi Sekizuka Fumio Seki Kouji Sakai Makoto Kuroda Ryoji Yamaguchi Makoto Takeda
author_sort	Noriyuki Otsuki
title	The V protein of canine distemper virus is required for virus replication in human epithelial cells.
title_short	The V protein of canine distemper virus is required for virus replication in human epithelial cells.
title_full	The V protein of canine distemper virus is required for virus replication in human epithelial cells.
title_fullStr	The V protein of canine distemper virus is required for virus replication in human epithelial cells.
title_full_unstemmed	The V protein of canine distemper virus is required for virus replication in human epithelial cells.
title_sort	v protein of canine distemper virus is required for virus replication in human epithelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3a25febd344543228c84950a70bfc556
work_keys_str_mv	AT noriyukiotsuki thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT yuichironakatsu thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT torukubota thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT tsuyoshisekizuka thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT fumioseki thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT koujisakai thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT makotokuroda thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT ryojiyamaguchi thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT makototakeda thevproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT noriyukiotsuki vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT yuichironakatsu vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT torukubota vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT tsuyoshisekizuka vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT fumioseki vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT koujisakai vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT makotokuroda vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT ryojiyamaguchi vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells AT makototakeda vproteinofcaninedistempervirusisrequiredforvirusreplicationinhumanepithelialcells
_version_	1718421439063785472

The V protein of canine distemper virus is required for virus replication in human epithelial cells.

Ejemplares similares