A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.

Kinesin-13s are microtubule (MT) depolymerases different from most other kinesins that move along MTs. Like other kinesins, they have a motor or head domain (HD) containing a tubulin and an ATP binding site. Interestingly, kinesin-13s have an additional binding site (Kin-Tub-2) on the opposite side...

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Autores principales: Dong Zhang, Ana B Asenjo, Michaela Greenbaum, Luping Xie, David J Sharp, Hernando Sosa
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Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/3a2e77f84f824dfabd3fd7f6445f5e4b
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spelling oai:doaj.org-article:3a2e77f84f824dfabd3fd7f6445f5e4b2021-11-18T08:57:50ZA second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.1932-620310.1371/journal.pone.0073075https://doaj.org/article/3a2e77f84f824dfabd3fd7f6445f5e4b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24015286/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Kinesin-13s are microtubule (MT) depolymerases different from most other kinesins that move along MTs. Like other kinesins, they have a motor or head domain (HD) containing a tubulin and an ATP binding site. Interestingly, kinesin-13s have an additional binding site (Kin-Tub-2) on the opposite side of the HD that contains several family conserved positively charged residues. The role of this site in kinesin-13 function is not clear. To address this issue, we investigated the in-vitro and in-vivo effects of mutating Kin-Tub-2 family conserved residues on the Drosophila melanogaster kinesin-13, KLP10A. We show that the Kin-Tub-2 site enhances tubulin cross-linking and MT bundling properties of KLP10A in-vitro. Disruption of the Kin-Tub-2 site, despite not having a deleterious effect on MT depolymerization, results in abnormal mitotic spindles and lagging chromosomes during mitosis in Drosophila S2 cells. The results suggest that the additional Kin-Tub-2 tubulin biding site plays a direct MT attachment role in-vivo.Dong ZhangAna B AsenjoMichaela GreenbaumLuping XieDavid J SharpHernando SosaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73075 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Dong Zhang
Ana B Asenjo
Michaela Greenbaum
Luping Xie
David J Sharp
Hernando Sosa
A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
description Kinesin-13s are microtubule (MT) depolymerases different from most other kinesins that move along MTs. Like other kinesins, they have a motor or head domain (HD) containing a tubulin and an ATP binding site. Interestingly, kinesin-13s have an additional binding site (Kin-Tub-2) on the opposite side of the HD that contains several family conserved positively charged residues. The role of this site in kinesin-13 function is not clear. To address this issue, we investigated the in-vitro and in-vivo effects of mutating Kin-Tub-2 family conserved residues on the Drosophila melanogaster kinesin-13, KLP10A. We show that the Kin-Tub-2 site enhances tubulin cross-linking and MT bundling properties of KLP10A in-vitro. Disruption of the Kin-Tub-2 site, despite not having a deleterious effect on MT depolymerization, results in abnormal mitotic spindles and lagging chromosomes during mitosis in Drosophila S2 cells. The results suggest that the additional Kin-Tub-2 tubulin biding site plays a direct MT attachment role in-vivo.
format article
author Dong Zhang
Ana B Asenjo
Michaela Greenbaum
Luping Xie
David J Sharp
Hernando Sosa
author_facet Dong Zhang
Ana B Asenjo
Michaela Greenbaum
Luping Xie
David J Sharp
Hernando Sosa
author_sort Dong Zhang
title A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
title_short A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
title_full A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
title_fullStr A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
title_full_unstemmed A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
title_sort second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/3a2e77f84f824dfabd3fd7f6445f5e4b
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