A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
Kinesin-13s are microtubule (MT) depolymerases different from most other kinesins that move along MTs. Like other kinesins, they have a motor or head domain (HD) containing a tubulin and an ATP binding site. Interestingly, kinesin-13s have an additional binding site (Kin-Tub-2) on the opposite side...
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2013
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oai:doaj.org-article:3a2e77f84f824dfabd3fd7f6445f5e4b2021-11-18T08:57:50ZA second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.1932-620310.1371/journal.pone.0073075https://doaj.org/article/3a2e77f84f824dfabd3fd7f6445f5e4b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24015286/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Kinesin-13s are microtubule (MT) depolymerases different from most other kinesins that move along MTs. Like other kinesins, they have a motor or head domain (HD) containing a tubulin and an ATP binding site. Interestingly, kinesin-13s have an additional binding site (Kin-Tub-2) on the opposite side of the HD that contains several family conserved positively charged residues. The role of this site in kinesin-13 function is not clear. To address this issue, we investigated the in-vitro and in-vivo effects of mutating Kin-Tub-2 family conserved residues on the Drosophila melanogaster kinesin-13, KLP10A. We show that the Kin-Tub-2 site enhances tubulin cross-linking and MT bundling properties of KLP10A in-vitro. Disruption of the Kin-Tub-2 site, despite not having a deleterious effect on MT depolymerization, results in abnormal mitotic spindles and lagging chromosomes during mitosis in Drosophila S2 cells. The results suggest that the additional Kin-Tub-2 tubulin biding site plays a direct MT attachment role in-vivo.Dong ZhangAna B AsenjoMichaela GreenbaumLuping XieDavid J SharpHernando SosaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73075 (2013) |
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Medicine R Science Q Dong Zhang Ana B Asenjo Michaela Greenbaum Luping Xie David J Sharp Hernando Sosa A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. |
description |
Kinesin-13s are microtubule (MT) depolymerases different from most other kinesins that move along MTs. Like other kinesins, they have a motor or head domain (HD) containing a tubulin and an ATP binding site. Interestingly, kinesin-13s have an additional binding site (Kin-Tub-2) on the opposite side of the HD that contains several family conserved positively charged residues. The role of this site in kinesin-13 function is not clear. To address this issue, we investigated the in-vitro and in-vivo effects of mutating Kin-Tub-2 family conserved residues on the Drosophila melanogaster kinesin-13, KLP10A. We show that the Kin-Tub-2 site enhances tubulin cross-linking and MT bundling properties of KLP10A in-vitro. Disruption of the Kin-Tub-2 site, despite not having a deleterious effect on MT depolymerization, results in abnormal mitotic spindles and lagging chromosomes during mitosis in Drosophila S2 cells. The results suggest that the additional Kin-Tub-2 tubulin biding site plays a direct MT attachment role in-vivo. |
format |
article |
author |
Dong Zhang Ana B Asenjo Michaela Greenbaum Luping Xie David J Sharp Hernando Sosa |
author_facet |
Dong Zhang Ana B Asenjo Michaela Greenbaum Luping Xie David J Sharp Hernando Sosa |
author_sort |
Dong Zhang |
title |
A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. |
title_short |
A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. |
title_full |
A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. |
title_fullStr |
A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. |
title_full_unstemmed |
A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. |
title_sort |
second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doaj.org/article/3a2e77f84f824dfabd3fd7f6445f5e4b |
work_keys_str_mv |
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