Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus

Abstract The sporangiophores of Phycomyces blakesleeanus have been used as a model system to study sensory transduction, helical growth, and to establish global biophysical equations for expansive growth of walled cells. More recently, local statistical biophysical models of the cell wall are being...

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Autores principales:	Joseph K. E. Ortega, Revathi P. Mohan, Cindy M. Munoz, Shankar Lalitha Sridhar, Franck J. Vernerey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a36073e4173466aaa38a1e4ab672d5f
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spelling	oai:doaj.org-article:3a36073e4173466aaa38a1e4ab672d5f2021-12-02T13:30:22ZHelical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus10.1038/s41598-021-83254-52045-2322https://doaj.org/article/3a36073e4173466aaa38a1e4ab672d5f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83254-5https://doaj.org/toc/2045-2322Abstract The sporangiophores of Phycomyces blakesleeanus have been used as a model system to study sensory transduction, helical growth, and to establish global biophysical equations for expansive growth of walled cells. More recently, local statistical biophysical models of the cell wall are being constructed to better understand the molecular underpinnings of helical growth and its behavior during the many growth responses of the sporangiophores to sensory stimuli. Previous experimental and theoretical findings guide the development of these local models. Future development requires an investigation of explicit and implicit assumptions made in the prior research. Here, experiments are conducted to test three assumptions made in prior research, that (a) elongation rate, (b) rotation rate, and (c) helical growth steepness, R, of the sporangiophore remain constant during the phototropic response (bending toward unilateral light) and the avoidance response (bending away from solid barriers). The experimental results reveal that all three assumptions are incorrect for the phototropic response and probably incorrect for the avoidance response but the results are less conclusive. Generally, the experimental results indicate that the elongation and rotation rates increase during these responses, as does R, indicating that the helical growth steepness become flatter. The implications of these findings on prior research, the “fibril reorientation and slippage” hypothesis, global biophysical equations, and local statistical biophysical models are discussed.Joseph K. E. OrtegaRevathi P. MohanCindy M. MunozShankar Lalitha SridharFranck J. VernereyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joseph K. E. Ortega Revathi P. Mohan Cindy M. Munoz Shankar Lalitha Sridhar Franck J. Vernerey Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus
description	Abstract The sporangiophores of Phycomyces blakesleeanus have been used as a model system to study sensory transduction, helical growth, and to establish global biophysical equations for expansive growth of walled cells. More recently, local statistical biophysical models of the cell wall are being constructed to better understand the molecular underpinnings of helical growth and its behavior during the many growth responses of the sporangiophores to sensory stimuli. Previous experimental and theoretical findings guide the development of these local models. Future development requires an investigation of explicit and implicit assumptions made in the prior research. Here, experiments are conducted to test three assumptions made in prior research, that (a) elongation rate, (b) rotation rate, and (c) helical growth steepness, R, of the sporangiophore remain constant during the phototropic response (bending toward unilateral light) and the avoidance response (bending away from solid barriers). The experimental results reveal that all three assumptions are incorrect for the phototropic response and probably incorrect for the avoidance response but the results are less conclusive. Generally, the experimental results indicate that the elongation and rotation rates increase during these responses, as does R, indicating that the helical growth steepness become flatter. The implications of these findings on prior research, the “fibril reorientation and slippage” hypothesis, global biophysical equations, and local statistical biophysical models are discussed.
format	article
author	Joseph K. E. Ortega Revathi P. Mohan Cindy M. Munoz Shankar Lalitha Sridhar Franck J. Vernerey
author_facet	Joseph K. E. Ortega Revathi P. Mohan Cindy M. Munoz Shankar Lalitha Sridhar Franck J. Vernerey
author_sort	Joseph K. E. Ortega
title	Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus
title_short	Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus
title_full	Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus
title_fullStr	Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus
title_full_unstemmed	Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus
title_sort	helical growth during the phototropic response, avoidance response, and in stiff mutants of phycomyces blakesleeanus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3a36073e4173466aaa38a1e4ab672d5f
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Helical growth during the phototropic response, avoidance response, and in stiff mutants of Phycomyces blakesleeanus

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