Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection

A Mohammed Fayaz,1,* Zhujun Ao,1,3,* Morkattu Girilal,2 Liyu Chen,3,4 Xianzhong Xiao,4 PT Kalaichelvan,2 Xiaojian Yao1,31Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada; 2CAS in Botany, University of M...

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Autores principales: Mohammed Fayaz A, Ao Z, Girilal M, Chen L, Xiao X, Kalaichelvan PT, Yao X
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Publicado: Dove Medical Press 2012
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spelling oai:doaj.org-article:3a397219a85b4e60a7c1aeedb106b9f52021-12-02T05:10:27ZInactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection1176-91141178-2013https://doaj.org/article/3a397219a85b4e60a7c1aeedb106b9f52012-09-01T00:00:00Zhttp://www.dovepress.com/inactivation-of-microbial-infectiousness-by-silver-nanoparticles-coate-a11087https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013A Mohammed Fayaz,1,* Zhujun Ao,1,3,* Morkattu Girilal,2 Liyu Chen,3,4 Xianzhong Xiao,4 PT Kalaichelvan,2 Xiaojian Yao1,31Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada; 2CAS in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India; 3Department of Microbiology, 4School of Basic Medical Sciences, Central South University, Changsha, Hunan, People’s Republic of China*Both authors contributed equally to this workAbstract: Recent research suggests that today’s condoms are only 85% effective in preventing human immunodeficiency virus (HIV) and other sexually transmitted diseases. In response, there has been a push to develop more effective ways of decreasing the spread of the disease. The new nanotechnology-based condom holds the promise of being more potent than the first-generation products. The preliminary goal of this study was to develop a silver nanoparticles (Ag-NPs)-coated polyurethane condom (PUC) and to investigate its antimicrobial potential including the inactivation of HIV and herpes simplex virus (HSV) infectiousness. The Ag-NPs-coated PUC was characterized by using ultraviolet-visible spectrophotometry, Fourier transform-infrared spectroscopy, high-resolution scanning electron microscopy, and energy-dispersive analysis of X-ray spectroscopy. Nanoparticles were stable on the PUC and not washed away by water. Morphology of the PUC was retained after coating. The NP binding is due to its interaction with the nitrogen atom of the PUC. No significant toxic effects was observed when human HeLa cells, 293T and C8166 T cells were contacted to Ag-NPs-coated PUC for three hours. Interestingly, our results demonstrated that the contact of the Ag-NPs-coated PUC with HIV-1 and HSV-1/2 was able to efficiently inactivate their infectiousness. In an attempt to elucidate the antiviral action of the Ag-NPs, we have demonstrated that the anti-HIV activity was primarily mediated by the Ag-NPs, which are associated with the PUC. In addition, the data showed that both macrophage (M)-tropic and T lymphocyte (T)-tropic strains of HIV-1 were highly sensitive to the Ag-NPs-coated PUC. Furthermore, we also showed that the Ag-NPs-coated PUC was able to inhibit the growth of bacteria and fungi. These results demonstrated that the Ag-NPs-coated PUC is able to directly inactivate the microbe’s infectious ability and provides another defense line against these sexually transmitted microbial infections.Keywords: silver nanoparticles, condom, HIV-1, HSV-1/2, antimicrobialMohammed Fayaz AAo ZGirilal MChen LXiao XKalaichelvan PTYao XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5007-5018 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Mohammed Fayaz A
Ao Z
Girilal M
Chen L
Xiao X
Kalaichelvan PT
Yao X
Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection
description A Mohammed Fayaz,1,* Zhujun Ao,1,3,* Morkattu Girilal,2 Liyu Chen,3,4 Xianzhong Xiao,4 PT Kalaichelvan,2 Xiaojian Yao1,31Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada; 2CAS in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India; 3Department of Microbiology, 4School of Basic Medical Sciences, Central South University, Changsha, Hunan, People’s Republic of China*Both authors contributed equally to this workAbstract: Recent research suggests that today’s condoms are only 85% effective in preventing human immunodeficiency virus (HIV) and other sexually transmitted diseases. In response, there has been a push to develop more effective ways of decreasing the spread of the disease. The new nanotechnology-based condom holds the promise of being more potent than the first-generation products. The preliminary goal of this study was to develop a silver nanoparticles (Ag-NPs)-coated polyurethane condom (PUC) and to investigate its antimicrobial potential including the inactivation of HIV and herpes simplex virus (HSV) infectiousness. The Ag-NPs-coated PUC was characterized by using ultraviolet-visible spectrophotometry, Fourier transform-infrared spectroscopy, high-resolution scanning electron microscopy, and energy-dispersive analysis of X-ray spectroscopy. Nanoparticles were stable on the PUC and not washed away by water. Morphology of the PUC was retained after coating. The NP binding is due to its interaction with the nitrogen atom of the PUC. No significant toxic effects was observed when human HeLa cells, 293T and C8166 T cells were contacted to Ag-NPs-coated PUC for three hours. Interestingly, our results demonstrated that the contact of the Ag-NPs-coated PUC with HIV-1 and HSV-1/2 was able to efficiently inactivate their infectiousness. In an attempt to elucidate the antiviral action of the Ag-NPs, we have demonstrated that the anti-HIV activity was primarily mediated by the Ag-NPs, which are associated with the PUC. In addition, the data showed that both macrophage (M)-tropic and T lymphocyte (T)-tropic strains of HIV-1 were highly sensitive to the Ag-NPs-coated PUC. Furthermore, we also showed that the Ag-NPs-coated PUC was able to inhibit the growth of bacteria and fungi. These results demonstrated that the Ag-NPs-coated PUC is able to directly inactivate the microbe’s infectious ability and provides another defense line against these sexually transmitted microbial infections.Keywords: silver nanoparticles, condom, HIV-1, HSV-1/2, antimicrobial
format article
author Mohammed Fayaz A
Ao Z
Girilal M
Chen L
Xiao X
Kalaichelvan PT
Yao X
author_facet Mohammed Fayaz A
Ao Z
Girilal M
Chen L
Xiao X
Kalaichelvan PT
Yao X
author_sort Mohammed Fayaz A
title Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection
title_short Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection
title_full Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection
title_fullStr Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection
title_full_unstemmed Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection
title_sort inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit hiv- and hsv-transmitted infection
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/3a397219a85b4e60a7c1aeedb106b9f5
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