Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells
BACKGROUND AND OBJECTIVE: Cancer is one of the challenges of the medical world. Recently, in the search for new compounds with anti-cancer properties, attention to natural compounds has increased. The aim of this experimental study was to investigate the anti-cancer properties of various concentrati...
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| Formato: | article |
| Lenguaje: | EN FA |
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Babol University of Medical Sciences
2021
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| Acceso en línea: | https://doaj.org/article/3a3b96c74ce84206928329018076c75a |
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| Sumario: | BACKGROUND AND OBJECTIVE: Cancer is one of the challenges of the medical world. Recently, in the search for new compounds with anti-cancer properties, attention to natural compounds has increased. The aim of this experimental study was to investigate the anti-cancer properties of various concentrations of aqueous and hydroalcoholic extracts of Zophobas morio (Coleoptera: Tenebrionidae) larvae, as a mass-rearing and available insect, on breast cancer cells.
METHODS: In this experimental study, the insect was reared at a temperature of 25 °C and a relative humidity of 60% on wheat bran. At the final larval stage, the insects were dried and pulverized. Breast cancer cells (MCF-7) and normal cells (HUVEC) purchased from Pasteur Institute, were treated for 24 and 72 hours by different concentrations of aqueous and hydroalcoholic extracts (zero, 25, 100, 400 and 1200 μg/ml). Water was used as a control. Cell viability was measured by MTT assay. In five treatment groups, 10,000 cells of each extract were cultured in three replicates.
FINDINGS: Adding high concentration of hydroalcoholic extract (39.42±4.48 in 24 hours, 23.52±1.82 in 72 hours) compared to aqueous extract (56.02±9.43 in 24 hours, 36.52±4.07 at 72 hours) had a better effect on inhibiting the proliferation of cancer cells (p<0.05). The effect of these extracts on normal cells was observed only in high concentrations and its amount was lower than cancer cells (aqueous and hydroalcoholic extracts [61.77±3.44 and 57.21±1.10 in 24 hours] and [39.25±6.53 and 27.14±1.38 in 72 hours], respectively) (p<0.05).
CONCLUSION: The results showed that the high concentration of hydroalcoholic extract of Z. morio larvae played an effective role in inhibiting breast cancer cells, without having a significant effect on normal cells. |
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