Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells
BACKGROUND AND OBJECTIVE: Cancer is one of the challenges of the medical world. Recently, in the search for new compounds with anti-cancer properties, attention to natural compounds has increased. The aim of this experimental study was to investigate the anti-cancer properties of various concentrati...
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Babol University of Medical Sciences
2021
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oai:doaj.org-article:3a3b96c74ce84206928329018076c75a2021-11-29T07:40:49ZComparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells1561-41072251-7170https://doaj.org/article/3a3b96c74ce84206928329018076c75a2021-03-01T00:00:00Zhttp://jbums.org/article-1-9838-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Cancer is one of the challenges of the medical world. Recently, in the search for new compounds with anti-cancer properties, attention to natural compounds has increased. The aim of this experimental study was to investigate the anti-cancer properties of various concentrations of aqueous and hydroalcoholic extracts of Zophobas morio (Coleoptera: Tenebrionidae) larvae, as a mass-rearing and available insect, on breast cancer cells. METHODS: In this experimental study, the insect was reared at a temperature of 25 °C and a relative humidity of 60% on wheat bran. At the final larval stage, the insects were dried and pulverized. Breast cancer cells (MCF-7) and normal cells (HUVEC) purchased from Pasteur Institute, were treated for 24 and 72 hours by different concentrations of aqueous and hydroalcoholic extracts (zero, 25, 100, 400 and 1200 μg/ml). Water was used as a control. Cell viability was measured by MTT assay. In five treatment groups, 10,000 cells of each extract were cultured in three replicates. FINDINGS: Adding high concentration of hydroalcoholic extract (39.42±4.48 in 24 hours, 23.52±1.82 in 72 hours) compared to aqueous extract (56.02±9.43 in 24 hours, 36.52±4.07 at 72 hours) had a better effect on inhibiting the proliferation of cancer cells (p<0.05). The effect of these extracts on normal cells was observed only in high concentrations and its amount was lower than cancer cells (aqueous and hydroalcoholic extracts [61.77±3.44 and 57.21±1.10 in 24 hours] and [39.25±6.53 and 27.14±1.38 in 72 hours], respectively) (p<0.05). CONCLUSION: The results showed that the high concentration of hydroalcoholic extract of Z. morio larvae played an effective role in inhibiting breast cancer cells, without having a significant effect on normal cells.M DarbemamiehL SoltaniBabol University of Medical Sciencesarticledarkling beetlesbreast cancer cellscytotoxicityaqueous and hydroalcoholic extracts.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 280-288 (2021) |
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darkling beetles breast cancer cells cytotoxicity aqueous and hydroalcoholic extracts. Medicine R Medicine (General) R5-920 |
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darkling beetles breast cancer cells cytotoxicity aqueous and hydroalcoholic extracts. Medicine R Medicine (General) R5-920 M Darbemamieh L Soltani Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells |
| description |
BACKGROUND AND OBJECTIVE: Cancer is one of the challenges of the medical world. Recently, in the search for new compounds with anti-cancer properties, attention to natural compounds has increased. The aim of this experimental study was to investigate the anti-cancer properties of various concentrations of aqueous and hydroalcoholic extracts of Zophobas morio (Coleoptera: Tenebrionidae) larvae, as a mass-rearing and available insect, on breast cancer cells.
METHODS: In this experimental study, the insect was reared at a temperature of 25 °C and a relative humidity of 60% on wheat bran. At the final larval stage, the insects were dried and pulverized. Breast cancer cells (MCF-7) and normal cells (HUVEC) purchased from Pasteur Institute, were treated for 24 and 72 hours by different concentrations of aqueous and hydroalcoholic extracts (zero, 25, 100, 400 and 1200 μg/ml). Water was used as a control. Cell viability was measured by MTT assay. In five treatment groups, 10,000 cells of each extract were cultured in three replicates.
FINDINGS: Adding high concentration of hydroalcoholic extract (39.42±4.48 in 24 hours, 23.52±1.82 in 72 hours) compared to aqueous extract (56.02±9.43 in 24 hours, 36.52±4.07 at 72 hours) had a better effect on inhibiting the proliferation of cancer cells (p<0.05). The effect of these extracts on normal cells was observed only in high concentrations and its amount was lower than cancer cells (aqueous and hydroalcoholic extracts [61.77±3.44 and 57.21±1.10 in 24 hours] and [39.25±6.53 and 27.14±1.38 in 72 hours], respectively) (p<0.05).
CONCLUSION: The results showed that the high concentration of hydroalcoholic extract of Z. morio larvae played an effective role in inhibiting breast cancer cells, without having a significant effect on normal cells. |
| format |
article |
| author |
M Darbemamieh L Soltani |
| author_facet |
M Darbemamieh L Soltani |
| author_sort |
M Darbemamieh |
| title |
Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells |
| title_short |
Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells |
| title_full |
Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells |
| title_fullStr |
Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells |
| title_full_unstemmed |
Comparison of the Effect of Different Concentrations of Aqueous and Hydroalcoholic Extracts of Zophobas Morio (Coleoptera: Tenebrionidae) Larvae on Breast Cancer Cells and Human Umbilical Vein Endothelial Cells |
| title_sort |
comparison of the effect of different concentrations of aqueous and hydroalcoholic extracts of zophobas morio (coleoptera: tenebrionidae) larvae on breast cancer cells and human umbilical vein endothelial cells |
| publisher |
Babol University of Medical Sciences |
| publishDate |
2021 |
| url |
https://doaj.org/article/3a3b96c74ce84206928329018076c75a |
| work_keys_str_mv |
AT mdarbemamieh comparisonoftheeffectofdifferentconcentrationsofaqueousandhydroalcoholicextractsofzophobasmoriocoleopteratenebrionidaelarvaeonbreastcancercellsandhumanumbilicalveinendothelialcells AT lsoltani comparisonoftheeffectofdifferentconcentrationsofaqueousandhydroalcoholicextractsofzophobasmoriocoleopteratenebrionidaelarvaeonbreastcancercellsandhumanumbilicalveinendothelialcells |
| _version_ |
1718407518167760896 |