Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers

Complex disease states, like bacterial chondronecrosis with osteomyelitis (BCO), not only result in physiological symptoms, such as lameness, but also a complex systemic reaction involving immune and growth factor responses. For the modern broiler (meat-type) chickens, BCO is an animal welfare, prod...

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Autores principales:	Alison Ramser, Elizabeth Greene, Robert Wideman, Sami Dridi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cytokines FGF BCO FHN lameness broilers Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3a3df54b474542329181806e8050d2bc
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spelling	oai:doaj.org-article:3a3df54b474542329181806e8050d2bc2021-11-25T17:12:21ZLocal and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers10.3390/cells101131742073-4409https://doaj.org/article/3a3df54b474542329181806e8050d2bc2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3174https://doaj.org/toc/2073-4409Complex disease states, like bacterial chondronecrosis with osteomyelitis (BCO), not only result in physiological symptoms, such as lameness, but also a complex systemic reaction involving immune and growth factor responses. For the modern broiler (meat-type) chickens, BCO is an animal welfare, production, and economic concern involving bacterial infection, inflammation, and bone attrition with a poorly defined etiology. It is, therefore, critical to define the key inflammatory and bone-related factors involved in BCO. In this study, the local bone and systemic blood profile of inflammatory modulators, cytokines, and chemokines was elucidated along with inflammasome and key FGF genes. BCO-affected bone showed increased expression of cytokines IL-1β, while BCO-affected blood expressed upregulated TNFα and IL-12. The chemokine profile revealed increased IL-8 expression in both BCO-affected bone and blood in addition to inflammasome NLRC5 being upregulated in circulation. The key FGF receptor, FGFR1, was significantly downregulated in BCO-affected bone. The exposure of two different bone cell types, hFOB and chicken primary chondrocytes, to plasma from BCO-affected birds, as well as recombinant TNFα, resulted in significantly decreased cell viability. These results demonstrate an expression of proinflammatory and bone-resorptive factors and their potential contribution to BCO etiology through their impact on bone cell viability. This unique profile could be used for improved non-invasive detection of BCO and provides potential targets for treatments.Alison RamserElizabeth GreeneRobert WidemanSami DridiMDPI AGarticlecytokinesFGFBCOFHNlamenessbroilersBiology (General)QH301-705.5ENCells, Vol 10, Iss 3174, p 3174 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytokines FGF BCO FHN lameness broilers Biology (General) QH301-705.5
spellingShingle	cytokines FGF BCO FHN lameness broilers Biology (General) QH301-705.5 Alison Ramser Elizabeth Greene Robert Wideman Sami Dridi Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers
description	Complex disease states, like bacterial chondronecrosis with osteomyelitis (BCO), not only result in physiological symptoms, such as lameness, but also a complex systemic reaction involving immune and growth factor responses. For the modern broiler (meat-type) chickens, BCO is an animal welfare, production, and economic concern involving bacterial infection, inflammation, and bone attrition with a poorly defined etiology. It is, therefore, critical to define the key inflammatory and bone-related factors involved in BCO. In this study, the local bone and systemic blood profile of inflammatory modulators, cytokines, and chemokines was elucidated along with inflammasome and key FGF genes. BCO-affected bone showed increased expression of cytokines IL-1β, while BCO-affected blood expressed upregulated TNFα and IL-12. The chemokine profile revealed increased IL-8 expression in both BCO-affected bone and blood in addition to inflammasome NLRC5 being upregulated in circulation. The key FGF receptor, FGFR1, was significantly downregulated in BCO-affected bone. The exposure of two different bone cell types, hFOB and chicken primary chondrocytes, to plasma from BCO-affected birds, as well as recombinant TNFα, resulted in significantly decreased cell viability. These results demonstrate an expression of proinflammatory and bone-resorptive factors and their potential contribution to BCO etiology through their impact on bone cell viability. This unique profile could be used for improved non-invasive detection of BCO and provides potential targets for treatments.
format	article
author	Alison Ramser Elizabeth Greene Robert Wideman Sami Dridi
author_facet	Alison Ramser Elizabeth Greene Robert Wideman Sami Dridi
author_sort	Alison Ramser
title	Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers
title_short	Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers
title_full	Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers
title_fullStr	Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers
title_full_unstemmed	Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers
title_sort	local and systemic cytokine, chemokine, and fgf profile in bacterial chondronecrosis with osteomyelitis (bco)-affected broilers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3a3df54b474542329181806e8050d2bc
work_keys_str_mv	AT alisonramser localandsystemiccytokinechemokineandfgfprofileinbacterialchondronecrosiswithosteomyelitisbcoaffectedbroilers AT elizabethgreene localandsystemiccytokinechemokineandfgfprofileinbacterialchondronecrosiswithosteomyelitisbcoaffectedbroilers AT robertwideman localandsystemiccytokinechemokineandfgfprofileinbacterialchondronecrosiswithosteomyelitisbcoaffectedbroilers AT samidridi localandsystemiccytokinechemokineandfgfprofileinbacterialchondronecrosiswithosteomyelitisbcoaffectedbroilers
_version_	1718412641550991360

Local and Systemic Cytokine, Chemokine, and FGF Profile in Bacterial Chondronecrosis with Osteomyelitis (BCO)-Affected Broilers

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