The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.

The interaction of the Gram-negative bacterium Stenotrophomonas maltophilia with eukaryotes can improve overall plant growth and health, but can also cause opportunistic infections in humans. While the quorum sensing molecule DSF (diffusible signal factor) is responsible for the regulation of phenot...

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Autores principales:	Peyman Alavi, Henry Müller, Massimiliano Cardinale, Christin Zachow, María B Sánchez, José Luis Martínez, Gabriele Berg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a4b6740d5294c8eaf7016cd48fc9f99
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spelling	oai:doaj.org-article:3a4b6740d5294c8eaf7016cd48fc9f992021-11-18T07:37:19ZThe DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.1932-620310.1371/journal.pone.0067103https://doaj.org/article/3a4b6740d5294c8eaf7016cd48fc9f992013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874407/?tool=EBIhttps://doaj.org/toc/1932-6203The interaction of the Gram-negative bacterium Stenotrophomonas maltophilia with eukaryotes can improve overall plant growth and health, but can also cause opportunistic infections in humans. While the quorum sensing molecule DSF (diffusible signal factor) is responsible for the regulation of phenotypes in pathogenic Stenotrophomonas, up until now, no beneficial effects were reported to be controlled by it. Our objective was to study the function of DSF in the plant growth promoting model strain S. maltophilia R551-3 using functional and transcriptomic analyses. For this purpose, we compared the wild-type strain with a mutant deficient in the rpfF (regulation of pathogenicity factors) gene that is essential for the synthesis of DSF. Oilseed rape seeds treated with the wild-type strain showed a statistically significant increase in germination rate compared with those treated with the rpfF mutant. Similarly, the wild-type strain exhibited better plant growth promotion and a greater efficiency in colonizing oilseed rape compared to the mutant strain. Moreover, only the wild-type was capable of forming structured cell aggregates both in vitro and in the rhizosphere, a characteristic mediated by DSF. Gene transcription analyses showed that numerous genes known to play a role in plant colonization (e.g. chemotaxis, cell motility, biofilm formation, multidrug efflux pumps) are controlled by the rpf/DSF system in S. maltophilia. In addition, we detected new potential functions of spermidine, primarily for both growth promotion and stress protection. Overall, our results showed a correspondence between the regulation of DSF and the positive interaction effect with the plant host.Peyman AlaviHenry MüllerMassimiliano CardinaleChristin ZachowMaría B SánchezJosé Luis MartínezGabriele BergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e67103 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peyman Alavi Henry Müller Massimiliano Cardinale Christin Zachow María B Sánchez José Luis Martínez Gabriele Berg The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.
description	The interaction of the Gram-negative bacterium Stenotrophomonas maltophilia with eukaryotes can improve overall plant growth and health, but can also cause opportunistic infections in humans. While the quorum sensing molecule DSF (diffusible signal factor) is responsible for the regulation of phenotypes in pathogenic Stenotrophomonas, up until now, no beneficial effects were reported to be controlled by it. Our objective was to study the function of DSF in the plant growth promoting model strain S. maltophilia R551-3 using functional and transcriptomic analyses. For this purpose, we compared the wild-type strain with a mutant deficient in the rpfF (regulation of pathogenicity factors) gene that is essential for the synthesis of DSF. Oilseed rape seeds treated with the wild-type strain showed a statistically significant increase in germination rate compared with those treated with the rpfF mutant. Similarly, the wild-type strain exhibited better plant growth promotion and a greater efficiency in colonizing oilseed rape compared to the mutant strain. Moreover, only the wild-type was capable of forming structured cell aggregates both in vitro and in the rhizosphere, a characteristic mediated by DSF. Gene transcription analyses showed that numerous genes known to play a role in plant colonization (e.g. chemotaxis, cell motility, biofilm formation, multidrug efflux pumps) are controlled by the rpf/DSF system in S. maltophilia. In addition, we detected new potential functions of spermidine, primarily for both growth promotion and stress protection. Overall, our results showed a correspondence between the regulation of DSF and the positive interaction effect with the plant host.
format	article
author	Peyman Alavi Henry Müller Massimiliano Cardinale Christin Zachow María B Sánchez José Luis Martínez Gabriele Berg
author_facet	Peyman Alavi Henry Müller Massimiliano Cardinale Christin Zachow María B Sánchez José Luis Martínez Gabriele Berg
author_sort	Peyman Alavi
title	The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.
title_short	The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.
title_full	The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.
title_fullStr	The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.
title_full_unstemmed	The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.
title_sort	dsf quorum sensing system controls the positive influence of stenotrophomonas maltophilia on plants.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3a4b6740d5294c8eaf7016cd48fc9f99
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The DSF quorum sensing system controls the positive influence of Stenotrophomonas maltophilia on plants.

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