Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency

The development of crop cultivars with high nitrogen use efficiency (NUE) under low-N fertilizer inputs is imperative for sustainable agriculture. However, there has been little research on the molecular mechanisms underlying enhanced resilience to low N in high-NUE plants. The comparison of the tra...

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Autores principales:	Xinbo Zhang, Quan Ma, Fujian Li, Yonggang Ding, Yuan Yi, Min Zhu, Jinfeng Ding, Chunyan Li, Wenshan Guo, Xinkai Zhu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	near-isogenic lines (NILs) nitrogen use efficiency (NUE) nitrogen deficiency RNA sequencing (RNA-seq) qRT-PCR Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3a4c64d057ce44419c30b403e341c297
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spelling	oai:doaj.org-article:3a4c64d057ce44419c30b403e341c2972021-11-25T16:47:14ZTranscriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency10.3390/biology101111262079-7737https://doaj.org/article/3a4c64d057ce44419c30b403e341c2972021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1126https://doaj.org/toc/2079-7737The development of crop cultivars with high nitrogen use efficiency (NUE) under low-N fertilizer inputs is imperative for sustainable agriculture. However, there has been little research on the molecular mechanisms underlying enhanced resilience to low N in high-NUE plants. The comparison of the transcriptional responses of genotypes contrasting for NUE will facilitate an understanding of the key molecular mechanism of wheat resilience to low-N stress. In the current study, the RNA sequencing (RNA-seq) technique was employed to investigate the genotypic difference in response to N deficiency between two wheat NILs (1Y, high-NUE, and 1W, low-NUE). In our research, high- and low-NUE wheat NILs showed different patterns of gene expression under N-deficient conditions, and these N-responsive genes were classified into two major classes, including “frontloaded genes” and “relatively upregulated genes”. In total, 103 and 45 genes were identified as frontloaded genes in high-NUE and low-NUE wheat, respectively. In summary, our study might provide potential directions for further understanding the molecular mechanism of high-NUE genotypes adapting to low-N stress.Xinbo ZhangQuan MaFujian LiYonggang DingYuan YiMin ZhuJinfeng DingChunyan LiWenshan GuoXinkai ZhuMDPI AGarticlenear-isogenic lines (NILs)nitrogen use efficiency (NUE)nitrogen deficiencyRNA sequencing (RNA-seq)qRT-PCRBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1126, p 1126 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	near-isogenic lines (NILs) nitrogen use efficiency (NUE) nitrogen deficiency RNA sequencing (RNA-seq) qRT-PCR Biology (General) QH301-705.5
spellingShingle	near-isogenic lines (NILs) nitrogen use efficiency (NUE) nitrogen deficiency RNA sequencing (RNA-seq) qRT-PCR Biology (General) QH301-705.5 Xinbo Zhang Quan Ma Fujian Li Yonggang Ding Yuan Yi Min Zhu Jinfeng Ding Chunyan Li Wenshan Guo Xinkai Zhu Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
description	The development of crop cultivars with high nitrogen use efficiency (NUE) under low-N fertilizer inputs is imperative for sustainable agriculture. However, there has been little research on the molecular mechanisms underlying enhanced resilience to low N in high-NUE plants. The comparison of the transcriptional responses of genotypes contrasting for NUE will facilitate an understanding of the key molecular mechanism of wheat resilience to low-N stress. In the current study, the RNA sequencing (RNA-seq) technique was employed to investigate the genotypic difference in response to N deficiency between two wheat NILs (1Y, high-NUE, and 1W, low-NUE). In our research, high- and low-NUE wheat NILs showed different patterns of gene expression under N-deficient conditions, and these N-responsive genes were classified into two major classes, including “frontloaded genes” and “relatively upregulated genes”. In total, 103 and 45 genes were identified as frontloaded genes in high-NUE and low-NUE wheat, respectively. In summary, our study might provide potential directions for further understanding the molecular mechanism of high-NUE genotypes adapting to low-N stress.
format	article
author	Xinbo Zhang Quan Ma Fujian Li Yonggang Ding Yuan Yi Min Zhu Jinfeng Ding Chunyan Li Wenshan Guo Xinkai Zhu
author_facet	Xinbo Zhang Quan Ma Fujian Li Yonggang Ding Yuan Yi Min Zhu Jinfeng Ding Chunyan Li Wenshan Guo Xinkai Zhu
author_sort	Xinbo Zhang
title	Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
title_short	Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
title_full	Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
title_fullStr	Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
title_full_unstemmed	Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency
title_sort	transcriptome analysis reveals different responsive patterns to nitrogen deficiency in two wheat near-isogenic lines contrasting for nitrogen use efficiency
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3a4c64d057ce44419c30b403e341c297
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Transcriptome Analysis Reveals Different Responsive Patterns to Nitrogen Deficiency in Two Wheat Near-Isogenic Lines Contrasting for Nitrogen Use Efficiency

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